In:
European Journal of Organic Chemistry, Wiley, Vol. 2012, No. 5 ( 2012-02), p. 1003-1007
Abstract:
Two recently identified ( S )‐selective ω‐transaminases (ω‐TAs) that originate from Paracoccus denitrificans (Strep‐PD‐ωTA, cloned with an N ‐terminal Strep ‐tag II) and Pseudomonas fluorescens (PF‐ωTA) were employed for the asymmetric amination of selected prochiral ketones. The substrates tested were transformed into optically pure amines ( 〉 99 % ee ) with high conversion (up to 〉 99 %). The ω‐TAs led to higher conversion in the absence of dimethyl sulfoxide as a cosolvent than in its presence (15 %, v/v). Additionally, it was shown that a His‐tagged recombinant transaminase from Vibrio fluvialis (His‐VF‐ωTA, cloned with an N ‐terminal His 6 ‐tag) showed for a single substrate, ethyl acetoacetate, significantly higher stereoselectivity for the amination compared to the corresponding commercial enzyme preparation ( 〉 99 vs. 50 %).
Type of Medium:
Online Resource
ISSN:
1434-193X
,
1099-0690
DOI:
10.1002/ejoc.v2012.5
DOI:
10.1002/ejoc.201101476
Language:
English
Publisher:
Wiley
Publication Date:
2012
detail.hit.zdb_id:
1475010-7
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