In:
PLOS Biology, Public Library of Science (PLoS), Vol. 21, No. 5 ( 2023-5-18), p. e3002127-
Abstract:
Receptors that distinguish the multitude of microbes surrounding plants in the environment enable dynamic responses to the biotic and abiotic conditions encountered. In this study, we identify and characterise a glycan receptor kinase, EPR3a, closely related to the exopolysaccharide receptor EPR3. Epr3a is up-regulated in roots colonised by arbuscular mycorrhizal (AM) fungi and is able to bind glucans with a branching pattern characteristic of surface-exposed fungal glucans. Expression studies with cellular resolution show localised activation of the Epr3a promoter in cortical root cells containing arbuscules. Fungal infection and intracellular arbuscule formation are reduced in epr3a mutants. In vitro , the EPR3a ectodomain binds cell wall glucans in affinity gel electrophoresis assays. In microscale thermophoresis (MST) assays, rhizobial exopolysaccharide binding is detected with affinities comparable to those observed for EPR3, and both EPR3a and EPR3 bind a well-defined β-1,3/β-1,6 decasaccharide derived from exopolysaccharides of endophytic and pathogenic fungi. Both EPR3a and EPR3 function in the intracellular accommodation of microbes. However, contrasting expression patterns and divergent ligand affinities result in distinct functions in AM colonisation and rhizobial infection in Lotus japonicus . The presence of Epr3a and Epr3 genes in both eudicot and monocot plant genomes suggest a conserved function of these receptor kinases in glycan perception.
Type of Medium:
Online Resource
ISSN:
1545-7885
DOI:
10.1371/journal.pbio.3002127
DOI:
10.1371/journal.pbio.3002127.g001
DOI:
10.1371/journal.pbio.3002127.g002
DOI:
10.1371/journal.pbio.3002127.g003
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10.1371/journal.pbio.3002127.g004
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10.1371/journal.pbio.3002127.g005
DOI:
10.1371/journal.pbio.3002127.g006
DOI:
10.1371/journal.pbio.3002127.g007
DOI:
10.1371/journal.pbio.3002127.g008
DOI:
10.1371/journal.pbio.3002127.g009
DOI:
10.1371/journal.pbio.3002127.s001
DOI:
10.1371/journal.pbio.3002127.s002
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10.1371/journal.pbio.3002127.s003
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10.1371/journal.pbio.3002127.s004
DOI:
10.1371/journal.pbio.3002127.s005
DOI:
10.1371/journal.pbio.3002127.s006
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10.1371/journal.pbio.3002127.s007
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10.1371/journal.pbio.3002127.s008
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10.1371/journal.pbio.3002127.s009
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10.1371/journal.pbio.3002127.s010
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10.1371/journal.pbio.3002127.s011
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10.1371/journal.pbio.3002127.s012
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10.1371/journal.pbio.3002127.s013
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10.1371/journal.pbio.3002127.s014
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10.1371/journal.pbio.3002127.s015
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10.1371/journal.pbio.3002127.s016
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10.1371/journal.pbio.3002127.s017
DOI:
10.1371/journal.pbio.3002127.s018
DOI:
10.1371/journal.pbio.3002127.s019
DOI:
10.1371/journal.pbio.3002127.s020
DOI:
10.1371/journal.pbio.3002127.s021
DOI:
10.1371/journal.pbio.3002127.s022
DOI:
10.1371/journal.pbio.3002127.s023
DOI:
10.1371/journal.pbio.3002127.s024
DOI:
10.1371/journal.pbio.3002127.r001
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10.1371/journal.pbio.3002127.r002
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10.1371/journal.pbio.3002127.r003
DOI:
10.1371/journal.pbio.3002127.r004
DOI:
10.1371/journal.pbio.3002127.r005
DOI:
10.1371/journal.pbio.3002127.r006
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2023
detail.hit.zdb_id:
2126773-X
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