In:
Cell Proliferation, Wiley, Vol. 50, No. 1 ( 2017-02)
Abstract:
Male germline stem cells ( mGSC s), also called spermatogonial stem cells ( SSC s), constantly generate spermatozoa in male animals. A number of preliminary studies on mechanisms of mGSC self‐renewal have previously been conducted, revealing that several factors are involved in this regulated process. The p38 MAPK pathway is widely conserved in multiple cell types in vivo , and plays an important role in cell proliferation, differentiation, inflammation and apoptosis. However, its role in self‐renewal of mGSC s has not hitherto been determined. Materials and methods Here, the mouse mGSC s were cultured and their identity was verified by semi‐ RT ‐ PCR , alkaline phosphatase ( AP ) staining and immunofluorescence staining. Then, the p38 MAPK pathway was blocked by p38 MAPK ‐specific inhibitor SB 202190. mGSC self‐renewal ability was then analysed by observation of morphology, cell number, cell growth analysis, TUNEL incorporation assay and cell cycle analysis. Results Results showed that mouse mGSC self‐renewal ability was significantly inhibited by SB 202190. Conclusions This study showed for the first time that the p38 MAPK pathway plays a key role in maintaining self‐renewal capacity of mouse mGSC s, which offers a new self‐renewal pathway for these cells and contributes to overall knowledge of the mechanisms of mGSC self‐renewal.
Type of Medium:
Online Resource
ISSN:
0960-7722
,
1365-2184
DOI:
10.1111/cpr.2017.50.issue-1
Language:
English
Publisher:
Wiley
Publication Date:
2017
detail.hit.zdb_id:
2019986-7
SSG:
12
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