In:
Journal of Crohn's and Colitis, Oxford University Press (OUP), Vol. 16, No. 9 ( 2022-09-08), p. 1447-1460
Kurzfassung:
Endoscopy and the use of faecal calprotectin [faecal CP] are among the least-favoured methods for assessing disease activity by inflammatory bowel disease [IBD] patients; the handling/processing of faecal samples is also impractical. Therefore, we sought to develop a novel neo-epitope serum calprotectin enzyme-linked immunosorbent assay [ELISA], CPa9-HNE, with the aim of quantifying neutrophil activity and neutrophil extracellular trap [NET] -osis and proposing a non-invasive method for monitoring disease activity in IBD patients. Methods In vitro cleavage was performed by mixing calprotectin [S100A9/S100A8] with human neutrophil elastase [HNE] , and a novel HNE-derived calprotectin neo-epitope [CPa9-HNE] was identified by mass spectrometry for ELISA development. The CPa9-HNE ELISA was quantified in supernatants from ex vivo activated neutrophils and serum samples from patients with ulcerative colitis [UC, n = 43] , Crohn’s disease [CD, n = 93], and healthy subjects [HS, n = 23] . For comparison, faecal CP and MRP8/14 biomarkers were also measured. Results CPa9-HNE was specific for activated neutrophils ex vivo. Serum CPa9-HNE levels were 4-fold higher in CD [p & lt;0.0001] and UC [p & lt;0.0001] patients than in HS. CPa9-HNE correlated well with the Simple Endoscopic Score [SES] -CD score [r = 0.61, p & lt;0.0001], MES [r = 0.46, p = 0.0141] , and the full Mayo score [r = 0.52, p = 0.0013]. CPa9-HNE was able to differentiate between CD and UC patients in endoscopic remission and moderate/severe disease activity (CD: area under the curve [AUC] = 0.82 [p = 0.0003], UC: AUC = 0.87 [p = 0.0004] ). The performance of CPa9-HNE was equipotent or slightly better than that of faecal CP. Conclusions Serum CPa9-HNE levels were highly associated with CD and UC patients. CPa9-HNE correlated with the SES-CD score and the full Mayo score, indicating a strong association with disease activity.
Materialart:
Online-Ressource
ISSN:
1873-9946
,
1876-4479
DOI:
10.1093/ecco-jcc/jjac047
Sprache:
Englisch
Verlag:
Oxford University Press (OUP)
Publikationsdatum:
2022
ZDB Id:
2389631-0
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