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  • 1
    In: International Journal of Urology, Wiley, Vol. 3, No. s1 ( 1996-01)
    Abstract: To investigate whether rBAT gene products function as a crystine transporter component or as a transport activator, we microinjected several C–terminal deletion mutants of rBAT cRNA into Xenopus oocytes, and measured transport activity for arginine, leucine and cystine in the presence and absence of sodium. Wild type rBAT significantly stimulated the uptake of all 3 amino acids 10–20 fold compared to control mutants. On the other hand, no mutant, except a Δ511–685 mutant, stimulated the uptake of these amino acids. However, the Δ511–685 mutant significantly increased the uptake of arginine. In the presence of sodium, the Δ511–685 mutant also increased the uptake of leucine. The Δ511–685 mutant did not stimulate crystine uptake in the presence and absence of sodium. Furthermore, inhibition of L–arginine uptake by L–homoserine was seen only in the presence of sodium. These results suggest that mutant rBAT stimulates the endogenous amino acid transport system y + in oocytes. Finally, rBAT gene products, as the primary cause of cystinuria, may function as activators of the amino acid transport system in renal brush border membrane.
    Type of Medium: Online Resource
    ISSN: 0919-8172 , 1442-2042
    URL: Issue
    Language: English
    Publisher: Wiley
    Publication Date: 1996
    detail.hit.zdb_id: 2009793-1
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  • 2
    Online Resource
    Online Resource
    Wiley ; 2023
    In:  Pediatrics International Vol. 65, No. 1 ( 2023-01)
    In: Pediatrics International, Wiley, Vol. 65, No. 1 ( 2023-01)
    Abstract: Developing continuous and labor‐saving sedation/agitation monitoring methods in ventilated children is important to avoid undesirable events such as unplanned extubation. The existing scales are often challenging to use. We therefore aimed to evaluate the feasibility of sedation/agitation monitoring using a wearable device with a built‐in accelerometer for ventilated children. Methods This prospective observational pilot study included children aged 15 years or less, admitted to the pediatric intensive care unit on mechanical ventilation after cardiac catheterization between December 2021 and April 2022. The wearable device with a built‐in accelerometer was attached to either of the upper limbs, and accelerations due to upper limb movements were measured for 2 h after admission or until extubation, whichever was earliest. Accelerations were measured at 0.02 s intervals, with the mean acceleration calculated for each 1 min interval. The State Behavioral Scale (SBS) was completed at 1 min intervals, with the SBS score (−1, 0, 1, or 2) compared with the mean acceleration. Results The study included 20 children with a median age of 12 months. The mean accelerations and SBS scores were positively correlated (Kendall's τ , 0.22; p 〈 0.001), with an increase in the median (interquartile range) acceleration from an SBS score of −1 through 2, as follows: SBS −1, 0.200 (0.151–0.232) m/s 2 ; SBS 0, 0.202 (0.190–0.235) m/s 2 ; SBS, 1, 0.312 (0.236–0.427) m/s 2 ; SBS 2, 0.455 (0.332–0.517) m/s 2 . No adverse events were observed. Conclusions This study showed that continuous, labor‐saving sedation/agitation monitoring of ventilated children was feasible using a wearable device with a built‐in accelerometer.
    Type of Medium: Online Resource
    ISSN: 1328-8067 , 1442-200X
    URL: Issue
    Language: English
    Publisher: Wiley
    Publication Date: 2023
    detail.hit.zdb_id: 2008621-0
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  • 3
    In: Biochemical Journal, Portland Press Ltd., Vol. 328, No. 2 ( 1997-12-01), p. 657-664
    Abstract: The Na+-independent dibasic and neutral amino acid transporter NBAT is among the least hydrophobic of mammalian amino acid transporters. The transporter contains one to four transmembrane domains and induces amino acid transport activity via a b0,+-like system when expressed in Xenopus oocytes. However, the physiological role of NBAT remains unclear. Complementary DNA clones encoding mouse NBAT have now been isolated. The expression of mouse NBAT in Xenopus oocytes also induced an obligatory amino acid exchange activity similar to that of the b0,+-like system. The amount of NBAT mRNA in mouse kidney increased during postnatal development, consistent with the increase in renal cystine and dibasic transport activity. Dietary aspartate induced a marked increase in cystine transport via the b0,+ system in mouse ileum. A high-aspartate diet also increased the amount of NBAT mRNA in mouse ileum. In the ileum of mice fed on the aspartate diet, the extent of cystine transport was further increased by preloading brush border membrane vesicles with lysine. Hybrid depletion of NBAT mRNA from ileal polyadenylated RNA revealed that the increase in cystine transport activity induced by the high-aspartate diet, as measured in Xenopus oocytes, was attributable to NBAT. These results demonstrate that mouse NBAT has an important role in cystine transport.
    Type of Medium: Online Resource
    ISSN: 0264-6021 , 1470-8728
    RVK:
    Language: English
    Publisher: Portland Press Ltd.
    Publication Date: 1997
    detail.hit.zdb_id: 1473095-9
    SSG: 12
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  • 4
    In: Biochemical Journal, Portland Press Ltd., Vol. 343, No. 3 ( 1999-11-01), p. 705-712
    Abstract: In a study of the rat intestinal Pi transport system, an activator protein for rat Na/Pi co-transport system (PiUS) was isolated and characterized. We also investigated the effects of restriction of vitamin D and Pi (two of the most important physiological and pathophysiological regulators of Pi absorption in the small intestine) on intestinal Pi transport activity and the expression of Na/Pi co-transporters that are expressed in rat small intestine. Rat PiUS encodes a 424-residue protein with a calculated molecular mass of 51463 Da. The microinjection of rat PiUS into Xenopus oocytes markedly stimulated Na+-dependent Pi co-transport activity. In rats fed with a low-Pi diet, Na+-dependent Pi co-transport activity was increased approx. 2-fold compared with that of rats fed a normal Pi diet. Kinetic studies demonstrated that this increased activity was due to an elevation of Vmax but not Km. The PiUS mRNA levels showed an approximate doubling in the rats fed with the low-Pi diet compared with those fed with the normal Pi diet. In addition, after the administration of 1,25-dihydroxyvitamin D3 [1,25-(OH)2D3] to vitamin D-deficient animals, the Pi uptake was significantly increased in the Na+-dependent component in the brush border membrane vesicle (BBMV) at 24 and 48 h. In addition, we found a further high-affinity Na/Pi co-transport system in the BBMV isolated from the vitamin D-replete animals. The levels of type III Na/Pi co-transporter PiT-2 mRNA were increased 24 and 48 h after 1,25-(OH)2D3 administration to vitamin D-deficent animals, whereas PiUS and the type IIb Na/Pi co-transporter mRNA levels were unchanged. In conclusion, we first cloned a rat activator protein, PiUS, and then studied its role along with that of other type III Na/Pi co-transporters. PiUS and PiT-2 might be important components in the regulation of the intestinal Pi transport system by Pi restriction and 1,25-(OH)2D3.
    Type of Medium: Online Resource
    ISSN: 0264-6021 , 1470-8728
    RVK:
    Language: English
    Publisher: Portland Press Ltd.
    Publication Date: 1999
    detail.hit.zdb_id: 1473095-9
    SSG: 12
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  • 5
    In: Journal of Neurochemistry, Wiley, Vol. 164, No. 5 ( 2023-03), p. 658-670
    Abstract: Sulfatide is a sulfated glycosphingolipid that is present abundantly in myelin sheaths of the brain and spinal cord. It is synthesized by a cerebroside sulfotransferase encoded by Gal3st1 , which catalyzes the transfer of sulfate from 3′‐phosphoadenylylsulfate to galactosylceramide. We previously reported that Gal3st1 gene expression in the spinal cord is up‐regulated 1 day after intraplantar injection of complete Freund's adjuvant (CFA), indicating that sulfatide is involved in inflammatory pain. In the present study, we found that intrathecal injection of sulfatide led to mechanical allodynia. Sulfatide caused levels of glial fibrillary acidic protein (GFAP) and nitric oxide in the spinal cord to increase. Mechanical allodynia induced by intrathecal injection of sulfatide was blocked by nitric oxide synthase inhibitors and by suppression of astrocyte activation by L‐α‐aminoadipate. These results suggest that sulfatide‐induced mechanical allodynia involved glial activation and nitric oxide production. Blocking selectin, a sulfatide‐binding protein, with bimosiamose attenuated sulfatide‐induced allodynia and ameliorated CFA‐induced mechanical allodynia during inflammatory pain. Finally, elevated levels of sulfatide concentration in the spinal cord were observed during CFA‐induced inflammatory pain. The elevated sulfatide levels enhanced selectin activation in the spinal cord, resulting in mechanical allodynia. Our data suggest that sulfatide‐selectin interaction plays a key role in inflammatory pain. image
    Type of Medium: Online Resource
    ISSN: 0022-3042 , 1471-4159
    URL: Issue
    Language: English
    Publisher: Wiley
    Publication Date: 2023
    detail.hit.zdb_id: 2020528-4
    SSG: 12
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  • 6
    In: Biochemical Journal, Portland Press Ltd., Vol. 324, No. 3 ( 1997-06-15), p. 927-934
    Abstract: Three λ phage clones encompassing the Na+/phosphate co-transporter (NaPi-3) gene and its 5′ flanking region were isolated from a human genomic DNA library. The gene comprises 13 exons and 12 introns and spans approx. 14 kb. All exon–intron junctions conform to the GT/AG rule. The major transcription-initiation site was determined by primer-extension analysis and is an adenosine residue 57 bp upstream of the 3′ end of the first exon. There is a typical TATA box 28 bp upstream of the major transcription-initiation site and various cis-acting elements, including a cAMP-responsive element, AP-1, AP-2 and SP-1 sites in the 5′ flanking region. This region also contains three direct-repeat-like sequences that resemble the consensus binding sequence for members of the steroid–thyroid hormone receptor superfamily, including vitamin D. Deletion analysis suggests that the region from nt-2409 to nt-1259 in the 5′ flanking region may be involved in kidney-specific gene expression. Vitamin D responsiveness of the NaPi-3 promoter was also detected in COS-7 cells co-transfected with a human vitamin D receptor expression vector. The presence of the three vitamin D receptor-responsive elements in the NaPi-3 promoter may be important in mediating the enhanced expression of the gene by 1,25-dihydroxyvitamin D3.
    Type of Medium: Online Resource
    ISSN: 0264-6021 , 1470-8728
    RVK:
    Language: English
    Publisher: Portland Press Ltd.
    Publication Date: 1997
    detail.hit.zdb_id: 1473095-9
    SSG: 12
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  • 7
    In: Chemistry – A European Journal, Wiley, Vol. 25, No. 35 ( 2019-06-21), p. 8173-8173
    Type of Medium: Online Resource
    ISSN: 0947-6539 , 1521-3765
    URL: Issue
    RVK:
    Language: English
    Publisher: Wiley
    Publication Date: 2019
    detail.hit.zdb_id: 1478547-X
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  • 8
    Online Resource
    Online Resource
    Springer Science and Business Media LLC ; 2023
    In:  Carbon Balance and Management Vol. 18, No. 1 ( 2023-05-18)
    In: Carbon Balance and Management, Springer Science and Business Media LLC, Vol. 18, No. 1 ( 2023-05-18)
    Abstract: Discussion on reducing emissions from deforestation in developing countries began at the United Nations Framework Convention on Climate Change (UNFCCC) Conference of the Parties in 2005, and the agenda for “reducing emissions from deforestation and forest degradation, and the role of conservation, sustainable management of forests and enhancement of forest carbon stocks in developing countries (REDD+)” was introduced under the UNFCCC. The REDD+ framework was developed with the expectation that it would significantly contribute to climate change mitigation at a relatively low cost and produce benefits for both developed and developing countries. Finance is a key element of REDD+ implementation, and many financial sources, approaches, and mechanisms have supported REDD+-related activities in various developing countries. However, the comprehensive challenges and lessons learned for REDD+ finance and its governance have not been fully explored. This paper reviews the relevant literature to understand the challenges for REDD+ finance and its governance in two areas—(1) REDD+ finance aligned with the UNFCCC and (2) REDD+-related finance outside the UNFCCC—which have developed differently and have different implications. This paper first identifies the six key elements of REDD+ finance and its governance across the two fields, and then reviews the related challenges and lessons learned with respect to public and private finance. The challenges for REDD+ finance and its governance aligned with the UNFCCC include enhancing the performance of REDD+ finance using mainly public finance, such as results-based finance and the jurisdictional approach. In contrast, the challenges regarding REDD+-related finance outside the UNFCCC include enhancing the engagement of the private sector in REDD+ finance, mainly targeting the project level, and the relationship between voluntary carbon markets and other investment and finance mechanisms. This paper also identifies the common challenges across REDD+ finance and its governance in the two fields. These challenges include the need to enhance linkages between REDD+ and other objectives, such as carbon neutrality/net-zero, deforestation-free supply chains, and nature-based solutions, as well as the need to develop learning systems for REDD+ finance.
    Type of Medium: Online Resource
    ISSN: 1750-0680
    Language: English
    Publisher: Springer Science and Business Media LLC
    Publication Date: 2023
    detail.hit.zdb_id: 2243512-8
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  • 9
    Online Resource
    Online Resource
    American Chemical Society (ACS) ; 2010
    In:  The Journal of Physical Chemistry C Vol. 114, No. 23 ( 2010-06-17), p. 10336-10341
    In: The Journal of Physical Chemistry C, American Chemical Society (ACS), Vol. 114, No. 23 ( 2010-06-17), p. 10336-10341
    Type of Medium: Online Resource
    ISSN: 1932-7447 , 1932-7455
    RVK:
    Language: English
    Publisher: American Chemical Society (ACS)
    Publication Date: 2010
    detail.hit.zdb_id: 2256522-X
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  • 10
    In: Nature Astronomy, Springer Science and Business Media LLC, Vol. 7, No. 2 ( 2022-12-12), p. 182-189
    Type of Medium: Online Resource
    ISSN: 2397-3366
    Language: English
    Publisher: Springer Science and Business Media LLC
    Publication Date: 2022
    detail.hit.zdb_id: 2879712-7
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