In:
Journal of Biomedical Materials Research Part A, Wiley, Vol. 102, No. 8 ( 2014-08), p. 2680-2691
Abstract:
Artificial nerve guidance conduits (NGCs) containing bioactive neurotrophic factors and topographical structure to biomimic native tissues are essential for efficient regeneration of nerve gaps. In this study, aligned SF/P(LLA‐CL) nanofibers encapsulating nerve growth factor (NGF), which was stabilized by SF in core, were fabricated via a coaxial electrospinning technique. The controlled release of NGF from the nanofibers was evaluated using enzyme‐linked immune sorbent assay (ELISA) and PC12 cell‐based bioassay over a 60‐day time period. The results demonstrated that NGF presented a sustained release and remained biological activity over 60 days. Nerve guidance conduits (NGCs) were fabricated by reeling the aligned SF/P(LLA‐CL) nanofibrous scaffolds encapsulating NGF and then used as a bridge implanted across a 15‐mm defect in the sciatic nerve of rats to promote nerve regeneration. The outcome in terms of regenerated nerve at 12 weeks was evaluated by a combination of electrophysiological assessment, histochemistry, and electron microscopy. All results clarified that the NGF‐encapsulated‐aligned SF/P(LLA‐CL) NGCs promoted peripheral nerve regeneration significantly better than the aligned SF/P(LLA‐CL) NGCs, suggesting that the released NGF from nanofibers could effectively promote the regeneration of peripheral nerve. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 102A: 2680–2691, 2014.
Type of Medium:
Online Resource
ISSN:
1549-3296
,
1552-4965
DOI:
10.1002/jbm.a.v102.8
Language:
English
Publisher:
Wiley
Publication Date:
2014
detail.hit.zdb_id:
1477192-5
SSG:
12
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