In:
Journal of Applied Physics, AIP Publishing, Vol. 102, No. 1 ( 2007-07-01)
Abstract:
A method is described for the label-free detection of DNA hybridization on porous silicon (por-Si), based upon the pairing of oligonucleotide chemistry and standard silicon nanotechnology. Por-Si with a pore diameter of approximately 30 nm was used to immobilize probe DNA. Infrared microspectroscopy was employed to monitor the hybridization of probe-DNA immobilized on pore surfaces with its complementary DNA (target-DNA). The immobilization of probe DNA on por-Si facilitates hybridization detection for a small sensing area (approximately 50×50 μm2) with a high detection efficiency. In this study, we fabricated a porous silicon-based DNA microarray (por-Si-microarray) using photolithographic and Si anodizing techniques. We demonstrate that DNA hybridization can be detected on a por-Si-microarray through the analysis of infrared absorption spectral profiles in the region where the vibration modes of the bases appear. This present approach demonstrates that por-Si-microarray in conjugation with infrared microspectroscopy has potential application in DNA sensing chips.
Type of Medium:
Online Resource
ISSN:
0021-8979
,
1089-7550
Language:
English
Publisher:
AIP Publishing
Publication Date:
2007
detail.hit.zdb_id:
220641-9
detail.hit.zdb_id:
3112-4
detail.hit.zdb_id:
1476463-5
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