In:
Cancer Science, Wiley, Vol. 98, No. 5 ( 2007-05), p. 659-664
Abstract:
By representational difference analysis, we previously identified the rat Erc (Expressed in renal carcinoma) gene that was more abundantly expressed in the renal carcinoma tissues of Eker rats than in the rat normal kidney. In this study, we raised antibodies against the amino‐terminal portion of the rat Erc, and demonstrated the existence of a ∼30‐kDa secretory form in the supernatant of cultured cells derived from rat renal carcinoma. The enzyme‐linked immunosorbent assay (ELISA) system using these antibodies detected high concentrations of this form in the sera of Eker rats bearing renal carcinomas, and in the sera of rats transplanted with mesothelioma cells. Mesothelin, a human homolog of the rat Erc, was recently reported to be a serum marker of malignant mesothelioma. The prognosis of mesothelioma is poor and there is no effective treatment at present. There are several rat model systems of mesothelioma that may be promising tools in the development of an antimesothelioma treatment. We hope our ELISA to detect the soluble form of rat Erc/Mesothelin is useful in the rat model system to exploit the antimesothelioma therapy to be used in human cases. ( Cancer Sci 2007; 98: 659–664)
Type of Medium:
Online Resource
ISSN:
1347-9032
,
1349-7006
DOI:
10.1111/cas.2007.98.issue-5
DOI:
10.1111/j.1349-7006.2007.00438.x
Language:
English
Publisher:
Wiley
Publication Date:
2007
detail.hit.zdb_id:
2115647-5
detail.hit.zdb_id:
2111204-6
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