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  • 1
    Online Resource
    Online Resource
    Multiplex RT Real-Time PCR Based on Target Failure to Detect and Identify Different Variants of SARS-CoV-2: A Feasible Method That Can Be Applied in Clinical Laboratories
    MDPI AG ; 2023
    In:  Diagnostics Vol. 13, No. 8 ( 2023-04-07), p. 1364-
    Details
    In: Diagnostics, MDPI AG, Vol. 13, No. 8 ( 2023-04-07), p. 1364-
    Abstract: Shortly after its emergence, Omicron and its sub-variants have quickly replaced the Delta variant during the current COVID-19 outbreaks in Vietnam and around the world. To enable the rapid and timely detection of existing and future variants for epidemiological surveillance and diagnostic applications, a robust, economical real-time PCR method that can specifically and sensitively detect and identify multiple different circulating variants is needed. The principle of target- failure (TF) real-time PCR is simple. If a target contains a deletion mutation, then there is a mismatch with the primer or probe, and the real-time PCR will fail to amplify the target. In this study, we designed and evaluated a novel multiplex RT real-time PCR (MPL RT-rPCR) based on the principle of target failure to detect and identify different variants of SARS-CoV-2 directly from the nasopharyngeal swabs collected from COVID-19 suspected cases. The primers and probes were designed based on the specific deletion mutations of current circulating variants. To evaluate the results from the MPL RT-rPCR, this study also designed nine pairs of primers for amplifying and sequencing of nine fragments from the S gene containing mutations of known variants. We demonstrated that (i) our MPL RT-rPCR was able to accurately detect multiple variants that existed in a single sample; (ii) the limit of detection of the MPL RT-rPCR in the detection of the variants ranged from 1 to 10 copies for Omicron BA.2 and BA.5, and from 10 to 100 copies for Delta, Omicron BA.1, recombination of BA.1 and BA.2, and BA.4; (iii) between January and September 2022, Omicron BA.1 emerged and co-existed with the Delta variant during the early period, both of which were rapidly replaced by Omicron BA.2, and this was followed by Omicron BA.5 as the dominant variant toward the later period. Our results showed that SARS-CoV-2 variants rapidly evolved within a short period of time, proving the importance of a robust, economical, and easy-to-access method not just for epidemiological surveillance but also for diagnoses around the world where SARS-CoV-2 variants remain the WHO’s highest health concern. Our highly sensitive and specific MPL RT-rPCR is considered suitable for further implementation in many laboratories, especially in developing countries.
    Type of Medium: Online Resource
    ISSN: 2075-4418
    URL: Article
    DOI: 10.3390/diagnostics13081364
    Language: English
    Publisher: MDPI AG
    Publication Date: 2023
    detail.hit.zdb_id: 2662336-5
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  • 2
    Online Resource
    Online Resource
    Cập nhật vai trò của thuốc ức chế SGLT2 trong dự phòng và điều trị suy tim
    Vietnam National Heart Association ; 2022
    In:  Tạp chí Tim mạch học Việt Nam , No. 99 ( 2022-11-30), p. 10-17
    Details
    In: Tạp chí Tim mạch học Việt Nam, Vietnam National Heart Association, , No. 99 ( 2022-11-30), p. 10-17
    Abstract: Cập nhật vai trò của thuốc ức chế SGLT2 trong dự phòng và điều trị suy tim
    Type of Medium: Online Resource
    ISSN: 1859-2848
    URL: Article
    DOI: 10.58354/jvc.99.2022.2
    Language: Unknown
    Publisher: Vietnam National Heart Association
    Publication Date: 2022
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  • 3
    Online Resource
    Online Resource
    Study on External Gas-Assisted Mold Temperature Control with the Assistance of a Flow Focusing Device in the Injection Molding Process
    MDPI AG ; 2021
    In:  Materials Vol. 14, No. 4 ( 2021-02-18), p. 965-
    Details
    In: Materials, MDPI AG, Vol. 14, No. 4 ( 2021-02-18), p. 965-
    Abstract: In the injection molding field, the flow of plastic material is one of the most important issues, especially regarding the ability of melted plastic to fill the thin walls of products. To improve the melt flow length, a high mold temperature was applied with pre-heating of the cavity surface. In this paper, we present our research on the injection molding process with pre-heating by external gas-assisted mold temperature control. After this, we observed an improvement in the melt flow length into thin-walled products due to the high mold temperature during the filling step. In addition, to develop the heating efficiency, a flow focusing device (FFD) was applied and verified. The simulations and experiments were carried out within an air temperature of 400 °C and heating time of 20 s to investigate a flow focusing device to assist with external gas-assisted mold temperature control (Ex-GMTC), with the application of various FFD types for the temperature distribution of the insert plate. The heating process was applied for a simple insert model with dimensions of 50 mm × 50 mm × 2 mm, in order to verify the influence of the FFD geometry on the heating result. After that, Ex-GMTC with the assistance of FFD was carried out for a mold-reading process, and the FFD influence was estimated by the mold heating result and the improvement of the melt flow length using acrylonitrile butadiene styrene (ABS). The results show that the air sprue gap (h) significantly affects the temperature of the insert and an air sprue gap of 3 mm gives the best heating rate, with the highest temperature being 321.2 °C. Likewise, the actual results show that the height of the flow focusing device (V) also influences the temperature of the insert plate and that a 5 mm high FFD gives the best results with a maximum temperature of 332.3 °C. Moreover, the heating efficiency when using FFD is always higher than without FFD. After examining the effect of FFD, its application was considered, in order to improve the melt flow length in injection molding, which increased from 38.6 to 170 mm, while the balance of the melt filling was also clearly improved.
    Type of Medium: Online Resource
    ISSN: 1996-1944
    URL: Article
    DOI: 10.3390/ma14040965
    Language: English
    Publisher: MDPI AG
    Publication Date: 2021
    detail.hit.zdb_id: 2487261-1
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  • 4
    Online Resource
    Online Resource
    A successful pilot application of the complex mixture surfactant polymer VPI SP to enhance oil recovery factor for the Lower Miocene, Bach Ho field
    Petrovietnam Journal ; 2022
    In:  Petrovietnam Journal Vol. 10 ( 2022-11-01), p. 19-27
    Details
    In: Petrovietnam Journal, Petrovietnam Journal, Vol. 10 ( 2022-11-01), p. 19-27
    Abstract: Enhanced oil recovery (EOR) implementation at field scale is complex. Therefore, pilot applications are usually conducted before field execution. This paper introduces a pilot project successfully applied for the Lower Miocene, Bach Ho field. Topics covered include: (i) pilot area selection, (ii) chemical preparation, (iii) specification and pilot design for execution, (iv) implementation, (v) pilot observation and interpretation, (vi) efficiency evaluation. The implementation of pilot projects is achieved on 23 January 2022. The evaluation shows that 2,700.2 tons of oil gained thanks to the application of the surfactant-polymer complex mixture (VPI SP).
    Type of Medium: Online Resource
    ISSN: 2615-9902
    URL: Article
    DOI: 10.47800/PVJ.2022.10-03
    Language: Unknown
    Publisher: Petrovietnam Journal
    Publication Date: 2022
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  • 5
    Online Resource
    Online Resource
    Numerical Study on the Flow Length in an Injection Molding Process with an External Air-Heating Step
    IOSR Journals ; 2017
    In:  International Journal of Engineering Research and Applications Vol. 07, No. 04 ( 2017-04), p. 85-89
    Details
    In: International Journal of Engineering Research and Applications, IOSR Journals, Vol. 07, No. 04 ( 2017-04), p. 85-89
    Type of Medium: Online Resource
    ISSN: 2248-9622 , 2248-9622
    URL: Issue
    URL: Journal
    URL: Article
    DOI: 10.9790/9622-070402
    DOI: 10.9790/9622
    DOI: 10.9790/9622-0704028589
    Language: Unknown
    Publisher: IOSR Journals
    Publication Date: 2017
    detail.hit.zdb_id: 2673348-1
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  • 6
    Online Resource
    Online Resource
    Optimizing 3D Printing Process Parameters for the Tensile Strength of Thermoplastic Polyurethane Plastic
    Springer Science and Business Media LLC ; 2023
    In:  Journal of Materials Engineering and Performance
    Details
    In: Journal of Materials Engineering and Performance, Springer Science and Business Media LLC
    Type of Medium: Online Resource
    ISSN: 1059-9495 , 1544-1024
    URL: Article
    DOI: 10.1007/s11665-023-07892-8
    Language: English
    Publisher: Springer Science and Business Media LLC
    Publication Date: 2023
    detail.hit.zdb_id: 2048384-3
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  • 7
    Online Resource
    Online Resource
    Influence of 3D Printing SLS Process Parameters on Product Tensile Strength
    Trans Tech Publications, Ltd. ; 2022
    In:  Defect and Diffusion Forum Vol. 417 ( 2022-06-28), p. 45-50
    Details
    In: Defect and Diffusion Forum, Trans Tech Publications, Ltd., Vol. 417 ( 2022-06-28), p. 45-50
    Abstract: Three-dimensional (3D) printing is a breakthrough technology that is being researched, developed and applied extensively in many areas of production around the world. One particular application is Selective Laser Sintering (SLS), in which a high-powered laser turns powdered material into a solid mass; however, in Vietnam the research and application of laser sintering is not very widespread, yet determining the durability, especially the tensile strength, of products made from this printing technology is necessary. The parameters during installation have a significant effect on the characteristics of the printing elements, so they need to they need to be studied. In this project, we focused on the influence of printing parameters to be able to optimize them to achieve the highest tensile strength in the products. The process consisted of producing specimens with 2 parameters to be tested: laser power and feed. The experimental results on the tensile strength were collected, and the influence of the printing parameters of the finished product were charted and analyzed. The result of the project is that laser power is the most important printing parameters that influences tensile strength.
    Type of Medium: Online Resource
    ISSN: 1662-9507
    URL: Issue
    URL: Article
    DOI: 10.4028/v-q6zs3y
    DOI: 10.4028/p-i92111
    Language: Unknown
    Publisher: Trans Tech Publications, Ltd.
    Publication Date: 2022
    detail.hit.zdb_id: 2051135-8
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  • 8
    Online Resource
    Online Resource
    Đánh giá hiệu quả thông khí áp lực dương không xâm nhập có hỗ trợ áp lực (BiPAP) trên bệnh nhân suy hô hấp do suy tim cấp mất bù
    108 Institute of Clinical Medical and Pharmaceutical Sciences ; 2022
    In:  Journal of 108 - Clinical Medicine and Phamarcy Vol. 17, No. 1 ( 2022-01-26)
    Details
    In: Journal of 108 - Clinical Medicine and Phamarcy, 108 Institute of Clinical Medical and Pharmaceutical Sciences, Vol. 17, No. 1 ( 2022-01-26)
    Abstract: Mục tiêu: Đánh giá hiệu quả của thông khí áp lực dương không xâm nhập có hỗ trợ áp lực (BiPAP) trên bệnh nhân suy hô hấp cấp do suy tim cấp mất bù. Đối tượng và phương pháp: Nghiên cứu mô tả 39 bệnh nhân suy hô hấp cấp do suy tim cấp mất bù không đáp ứng với điều trị nội khoa tiêu chuẩn được hỗ trợ hô hấp bằng thông khí áp lực dương không xâm nhập tại Khoa Hồi sức tim mạch, Bệnh viện Trung ương Quân đội 108 từ tháng 6/2020 đến tháng 11/2020. Kết quả: Trong 39 bệnh nhân được thông khí áp lực dương không xâm nhập, có 3 bệnh nhân phải chuyển đặt ống nội khí quản thở máy xâm nhập (7,7%), thời gian thở máy không xâm trung bình là 153,46 ± 47,16 phút, thời gian nằm viện trung bình 10,51 ± 6,03 ngày. Các thông số lâm sàng và khí máu cải thiện có ý nghĩa thống kê ngay trong giờ đầu. Biến chứng do thông khí áp lực dương không xâm nhập: Tổn thương da mặt, khô mắt (23,1%), trào ngược dạ dày - thực quản (12,8%), viêm phổi (7,7%), tụt huyết áp (7,7%). Thời gian thở máy không xâm nhập có mối tương quan thuận mức độ vừa với pH ban đầu (r = 0,392, p 〈 0,05) và tương quan nghịch mức độ vừa pCO2 ban đầu (r = -0,459, p 〈 0,01). Kết luận: Thông khí áp lực dương không xâm nhập là một phương pháp điều trị an toàn và hiệu quả, nên được áp dụng rộng rãi cho bệnh nhân suy tim cấp mất bù. Thời gian thở máy có liên quan với pH và pCO2 máu động mạch
    Type of Medium: Online Resource
    ISSN: 1859-2872
    URL: Issue
    URL: Article
    DOI: 10.52389/ydls.v17i1
    DOI: 10.52389/ydls.v17i1.1049
    Language: Unknown
    Publisher: 108 Institute of Clinical Medical and Pharmaceutical Sciences
    Publication Date: 2022
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  • 9
    Online Resource
    Online Resource
    WAAM Technique: Process Parameters Affecting the Mechanical Properties and Microstructures of Low-Carbon Steel
    MDPI AG ; 2023
    In:  Metals Vol. 13, No. 5 ( 2023-04-30), p. 873-
    Details
    In: Metals, MDPI AG, Vol. 13, No. 5 ( 2023-04-30), p. 873-
    Abstract: This study surveys the influences of travel speed, voltage, and intensity on the characteristics of low-carbon steel samples generated by the Wire Arc Additive Manufacturing (WAAM) technique. The results indicated that the WAAM samples have isotropy grain shape, with grain size number values varying from about 8 to 12. Interestingly, the WAAM sample achieves better mechanical properties with a higher ultimate tensile strength (UTS) value and higher elongation at break value than the original wire. The UTS value of the WAAM sample is 21–40% higher than the original steel wire. The WAAM sample with a travel rate of 350 mm·min−1, a voltage of 24 V, and an electrical intensity of 120 A reaches the highest UTS value of 694 MPa. The WAAM sample with a travel rate of 400 mm·min−1, a voltage of 22 V, and an electrical intensity of 170 A gains the lowest UTS value of 599 MPa. Moreover, the elongation values oscillate around 41–57%, two or three times higher than the original steel wire. SEM microstructure reveals a ductile fracture surface with dimples of the samples after the tensile test, indicating the toughness of the samples. The fracture surface also shows the equiaxial shape and grain size of the WAAM samples. According to Taguchi analyses, the travel rate factor greatly impacts grain size. The voltage factor has the highest effect on the UTS value. The intensity factor has the most significant impact on the elongation value.
    Type of Medium: Online Resource
    ISSN: 2075-4701
    URL: Article
    DOI: 10.3390/met13050873
    Language: English
    Publisher: MDPI AG
    Publication Date: 2023
    detail.hit.zdb_id: 2662252-X
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  • 10
    Online Resource
    Online Resource
    Trajectory Strategy Effects on the Material Characteristics in the WAAM Technique
    MDPI AG ; 2023
    In:  Micromachines Vol. 14, No. 4 ( 2023-04-08), p. 827-
    Details
    In: Micromachines, MDPI AG, Vol. 14, No. 4 ( 2023-04-08), p. 827-
    Abstract: The wire Arc Additive Manufacturing (WAAM) technique has evolved into a cutting-edge 3D printing technique. This study surveys the influences of trajectory on the characteristics of low-carbon steel samples generated by the WAAM technique. The results show that the grains in the WAAM samples are isotropic, with grain size numbers ranging from 7 to 12. Strategy 3, with a spiral trajectory, has the smallest grain size, while strategy 2, with a lean zigzag trajectory, has the largest. The variations in grain size are caused by differences in heat input and output during the printing process. The WAAM samples achieve a significantly higher UTS value than the original wire, demonstrating the WAAM technique’s benefit. Strategy 3, with a spiral trajectory, achieves the highest UTS value, 616.5 MPa, 24% higher than the original wire. The UTS values of strategy 1 (horizontal zigzag trajectory) and strategy 4 (curve zigzag trajectory) are comparable. WAAM samples have significantly higher elongation values than the original wire, with only 22% elongation. The sample with the highest elongation value, 47.2%, was produced by strategy 3. Strategy 2 has an elongation value of 37.9%. The value of elongation is proportional to the value of UTS. WAAM samples have average elastic modulus values of 95.8 GPa, 173.3 GPa, 92.2 GPa, and 83.9 GPa, corresponding to strategies 1, 2, 3, and 4. Only a strategy 2 sample has a similar elastic modulus value to the original wire. All samples have dimples on the fracture surface, indicating that the WAAM samples are ductile. These fracture surfaces’ equiaxial shape corresponds to the original microstructure’s equiaxial shape. The results provide the optimal trajectory for the WAAM products is the spiral trajectory, while the lean zigzag trajectory gains only modest characteristics.
    Type of Medium: Online Resource
    ISSN: 2072-666X
    URL: Article
    DOI: 10.3390/mi14040827
    Language: English
    Publisher: MDPI AG
    Publication Date: 2023
    detail.hit.zdb_id: 2620864-7
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