In:
The Journal of Immunology, The American Association of Immunologists, Vol. 190, No. 1_Supplement ( 2013-05-01), p. 62.14-62.14
Abstract:
UVB exposure of the skin is associated with amelioration of inflammatory diseases, which has been shown to be mediated by activated Tregs. Since we have recently shown that Tregs, for optimal functioning, are dependent on signals induced by the EGF-like growth factor AREG, we tested whether UVB-mediated immunosuppression is dependent on AREG. We irradiated C57BL/6 wt and AREG-/- mice with UVB light and sensitized them with DNFB. Mice then were challenged and ear swelling was measured. Our results show that UVB light irradiation suppressed ear swelling in wt but not in AREG-/- mice. To determine at what point AREG expression would be essential, we transferred lymphocytes isolated from lymph nodes of irradiated and sensitized wt mice into naïve wt or AREG-/- mice, that then were sensitized with DNFB and challenged. In contrast to wt recipients, AREG-/- mice lacked suppression upon challenge. Similar results were found when lymphocytes, derived from UVB-irradiated CD4cre x EGF-Rflox/flox or wt mice, were transferred into wt mice. Mice that had received lymphocytes from irradiated and sensitized wt mice were fully able to suppress challenge induced ear swelling, while those that had received lymphocytes derived from UVB-irradiated CD4cre x EGF-Rflox/flox showed a significantly decreased ability to suppress swelling. Our data shows that AREG at the site of inflammation is essential for UVB irradiation-induced and Treg mediated immunosuppression.
Type of Medium:
Online Resource
ISSN:
0022-1767
,
1550-6606
DOI:
10.4049/jimmunol.190.Supp.62.14
Language:
English
Publisher:
The American Association of Immunologists
Publication Date:
2013
detail.hit.zdb_id:
1475085-5
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