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  • 1
    In: Insects, MDPI AG, Vol. 11, No. 10 ( 2020-10-12), p. 691-
    Abstract: Grape phylloxera is one of the most dangerous insect pests for worldwide viticulture. The leaf- and root-galling phylloxerid has been managed by grafting European grapevines onto American rootstock hybrids. Recent reports pinpoint the appearance of host-adapted biotypes, but information about the biomolecular characteristics underlying grape phylloxera biotypisation and its role in host performance is scarce. Using RNA-sequencing, we sequenced the transcriptome of two larval stages: L1 (probing) and L2-3 (feeding) larvae of two root-feeding grape phylloxera lineages feeding on the rootstock Teleki 5C (biotype C) and V. vinifera Riesling (biotype A). In total, 7501 differentially expressed genes (DEGs) were commonly modulated by the two biotypes. For the probing larvae, we found an increased number of DEGs functionally associated with insect chemoreception traits, such as odorant-binding proteins, chemosensory proteins, ionotropic, odorant, and gustatory receptors. The transcriptomic profile of feeding larvae was enriched with DEGs associated with the primary metabolism. Larvae feeding on the tolerant rootstock Teleki 5C exhibited higher numbers of plant defense suppression-associated DEGs than larvae feeding on the susceptible host. Based on the identified DEGs, we discuss their potential role for the compatible grape phylloxera–Vitis interaction belowground. This study was the first to compare the transcriptomes of two grape phylloxera lineages feeding on a tolerant and susceptible host, respectively, and to identify DEGs involved in the molecular interaction with these hosts. Our data provide a source for future studies on host adaptation mechanisms of grape phylloxera and help to elucidate grape phylloxera resistance further.
    Type of Medium: Online Resource
    ISSN: 2075-4450
    Language: English
    Publisher: MDPI AG
    Publication Date: 2020
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  • 2
    In: Plant Systematics and Evolution, Springer Science and Business Media LLC, Vol. 299, No. 2 ( 2013-2), p. 357-367
    Type of Medium: Online Resource
    ISSN: 0378-2697 , 1615-6110
    RVK:
    Language: English
    Publisher: Springer Science and Business Media LLC
    Publication Date: 2013
    detail.hit.zdb_id: 7485-8
    detail.hit.zdb_id: 1463027-8
    detail.hit.zdb_id: 2781142-6
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  • 3
    In: BMC Ecology and Evolution, Springer Science and Business Media LLC, Vol. 21, No. 1 ( 2021-06-07)
    Abstract: Nile tilapia, Oreochromis niloticus (Linnaeus, 1758) is among the economically most important freshwater fish species in East Africa, and a major source of protein for local consumption. Human induced translocations of non-native stocks for aquaculture and fisheries have been found as a potential threat to the genetic diversity and integrity of local populations. In the present study, we investigate the genetic structure of O. niloticus from 16 waterbodies across Ethiopia using 37 microsatellite loci with SSR-GBAS techniques. Results The samples are structured into three main clusters shaped either by biogeographic factors or stocking activities. High F ST values (Global F ST  = 0.438) between populations indicate a high level of genetic differentiation and may suggest long term isolation even within the same drainage systems. Natural populations of the Omo-Turkana system and the lakes in the Southern Main Ethiopian Rift showed the highest genetic variability while low variability was found in stocked populations of lakes Hora, Hashenge and Hayq. Conclusions The results presented herein, may provide an essential basis for the management and conservation of the unique genetic resources in northern East Africa, and advance our understanding of biodiversity, phylogeny, evolution and development towards phylogenetically more accurate taxonomic classifications.
    Type of Medium: Online Resource
    ISSN: 2730-7182
    Language: English
    Publisher: Springer Science and Business Media LLC
    Publication Date: 2021
    detail.hit.zdb_id: 3053924-9
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  • 4
    In: BMC Ecology and Evolution, Springer Science and Business Media LLC, Vol. 22, No. 1 ( 2022-11-04)
    Abstract: As in most bryozoans, taxonomy and systematics of species in the genus Reteporella Busk, 1884 (family Phidoloporidae) has hitherto almost exclusively been based on morphological characters. From the central North Atlantic Azores Archipelago, nine Reteporella species have historically been reported, none of which have as yet been revised. Aiming to characterise the diversity and biogeographic distribution of Azorean Reteporella species, phylogenetic reconstructions were conducted on a dataset of 103 Azorean Reteporella specimens, based on the markers cytochrome C oxidase subunit 1, small and large ribosomal RNA subunits. Morphological identification was based on scanning electron microscopy and complemented the molecular inferences. Results Our results reveal two genetically distinct Azorean Reteporella clades, paraphyletic to eastern Atlantic and Mediterranean taxa. Moreover, an overall concordance between morphological and molecular species can be shown, and the actual bryozoan diversity in the Azores is greater than previously acknowledged as the dataset comprises three historically reported species and four putative new taxa, all of which are likely to be endemic. The inclusion of Mediterranean Reteporella specimens also revealed new species in the Adriatic and Ligurian Sea, whilst the inclusion of additional phidoloporid taxa hints at the non-monophyly of the genus Reteporella . Conclusion Being the first detailed genetic study on the genus Reteporella , the high divergence levels inferred within the genus Reteporella and family Phidoloporidae calls for the need of further revision. Nevertheless, the overall concordance between morphospecies and COI data suggest the potential adequacy of a 3% cut-off to distinguish Reteporella species. The discovery of new species in the remote Azores Archipelago as well as in the well-studied Mediterranean Sea indicates a general underestimation of bryozoan diversity. This study constitutes yet another example of the importance of integrative taxonomical approaches on understudied taxa, contributing to cataloguing genetic and morphological diversity.
    Type of Medium: Online Resource
    ISSN: 2730-7182
    Language: English
    Publisher: Springer Science and Business Media LLC
    Publication Date: 2022
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  • 5
    In: BMC Ecology and Evolution, Springer Science and Business Media LLC, Vol. 24, No. 1 ( 2024-01-02)
    Abstract: Habitat niches of fish species can exert a strong influence on population structure, even on a small geographical scale. In this scope, Pelasgus thesproticus is a great model species to study connectivity in riverine environments owing to its naturally patchy habitat distribution. Furthermore, it is important to conduct such studies in near-natural systems to avoid the impact of human disturbances on the river, such as fragmentation, morphological changes and habitat degradation. In this sense, the Vjosa in Albania is an excellent study area. A total of 204 individuals were sampled from five locations in the lower Vjosa and two tributaries and genotyped with 33 newly designed microsatellites loci using high throughput sequencing. The application of microsatellite genotyping by sequencing revealed genetic structure and some differentiation, even at a small spatial scale ( 〈 65 river km). A total of 500 alleles were found with an average of 0.93 private alleles among sites with rather low F ST values ( 〈 0.04). The extent of admixture observed in some populations indicate that the genetic structure is mainly influenced by upstream populations, either from the main river itself or from tributaries. In addition, the connection between a tributary and the other sites is disrupted by the flow regime, which is reflected in a high degree of divergence from the other populations. Our results indicate that hydrological conditions of the flowing river present strong barriers to gene flow, particularly in the upstream direction, but at the same time act as dispersal corridors in the downstream direction and exhibit source-sink dynamics in which upstream populations contribute disproportionately to downstream populations for this habitat specialist along the river. It is suggested that processes of colonization and reinforcement may play an important role in shaping the genetic structure of patchily distributed fish species in natural river systems. Future studies should increase the knowledge of dispersal factors, habitat heterogeneity, consequence of source-sink dynamics, and gene flow within the system, which will help to understand and maintain important processes related to metapopulation theory and the potential evolutionary consequences of habitat loss and fragmentation.
    Type of Medium: Online Resource
    ISSN: 2730-7182
    Language: English
    Publisher: Springer Science and Business Media LLC
    Publication Date: 2024
    detail.hit.zdb_id: 3053924-9
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  • 6
    Online Resource
    Online Resource
    Springer Science and Business Media LLC ; 2006
    In:  Biological Invasions Vol. 8, No. 6 ( 2006-10-17), p. 1355-1366
    In: Biological Invasions, Springer Science and Business Media LLC, Vol. 8, No. 6 ( 2006-10-17), p. 1355-1366
    Type of Medium: Online Resource
    ISSN: 1387-3547 , 1573-1464
    Language: English
    Publisher: Springer Science and Business Media LLC
    Publication Date: 2006
    detail.hit.zdb_id: 2014991-8
    SSG: 12
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  • 7
    In: Biochemical Genetics, Springer Science and Business Media LLC, Vol. 49, No. 11-12 ( 2011-12), p. 715-734
    Type of Medium: Online Resource
    ISSN: 0006-2928 , 1573-4927
    Language: English
    Publisher: Springer Science and Business Media LLC
    Publication Date: 2011
    detail.hit.zdb_id: 1496197-0
    SSG: 12
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  • 8
    Online Resource
    Online Resource
    Elsevier BV ; 2014
    In:  Ecological Engineering Vol. 71 ( 2014-10), p. 301-307
    In: Ecological Engineering, Elsevier BV, Vol. 71 ( 2014-10), p. 301-307
    Type of Medium: Online Resource
    ISSN: 0925-8574
    Language: English
    Publisher: Elsevier BV
    Publication Date: 2014
    detail.hit.zdb_id: 2000805-3
    detail.hit.zdb_id: 1127407-4
    SSG: 12
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  • 9
    Online Resource
    Online Resource
    MDPI AG ; 2021
    In:  Plants Vol. 10, No. 12 ( 2021-12-05), p. 2674-
    In: Plants, MDPI AG, Vol. 10, No. 12 ( 2021-12-05), p. 2674-
    Abstract: Recent progress in machine learning and deep learning has enabled the implementation of plant and crop detection using systematic inspection of the leaf shapes and other morphological characters for identification systems for precision farming. However, the models used for this approach tend to become black-box models, in the sense that it is difficult to trace characters that are the base for the classification. The interpretability is therefore limited and the explanatory factors may not be based on reasonable visible characters. We investigate the explanatory factors of recent machine learning and deep learning models for plant classification tasks. Based on a Daucus carota and a Beta vulgaris image data set, we implement plant classification models and compare those models by their predictive performance as well as explainability. For comparison we implemented a feed forward convolutional neuronal network as a default model. To evaluate the performance, we trained an unsupervised Bayesian Gaussian process latent variable model as well as a convolutional autoencoder for feature extraction and rely on a support vector machine for classification. The explanatory factors of all models were extracted and analyzed. The experiments show, that feed forward convolutional neuronal networks (98.24% and 96.10% mean accuracy) outperforms the Bayesian Gaussian process latent variable pipeline (92.08% and 94.31% mean accuracy) as well as the convolutional autoenceoder pipeline (92.38% and 93.28% mean accuracy) based approaches in terms of classification accuracy, even though not significant for Beta vulgaris images. Additionally, we found that the neuronal network used biological uninterpretable image regions for the plant classification task. In contrast to that, the unsupervised learning models rely on explainable visual characters. We conclude that supervised convolutional neuronal networks must be used carefully to ensure biological interpretability. We recommend unsupervised machine learning, careful feature investigation, and statistical feature analysis for biological applications.
    Type of Medium: Online Resource
    ISSN: 2223-7747
    Language: English
    Publisher: MDPI AG
    Publication Date: 2021
    detail.hit.zdb_id: 2704341-1
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  • 10
    Online Resource
    Online Resource
    Pensoft Publishers ; 2019
    In:  Biodiversity Information Science and Standards Vol. 3 ( 2019-07-04)
    In: Biodiversity Information Science and Standards, Pensoft Publishers, Vol. 3 ( 2019-07-04)
    Abstract: For computer vision based appraoches such as image classification (Krizhevsky et al. 2012), object detection (Ren et al. 2015) or pixel-wise weed classification (Milioto et al. 2017) machine learning is used for both feature extraction and processing (e.g. classification or regression). Historically, feature extraction (e.g. PCA; Ch. 12.1. in Bishop 2006) and processing were sequential and independent tasks (Wöber et al. 2013). Since the rise of convolutional neuronal networks (LeCun et al. 1989), a deep machine learning approach optimized for images, in 2012 (Krizhevsky et al. 2012), feature extraction for image analysis became an automated procedure. A convolutional neuronal net uses a deep architecture of artificial neurons (Goodfellow 2016) for both feature extraction and processing. Based on prior information such as image classes and supervised learning procedures, parameters of the neuronal nets are adjusted. This is known as the learning process. Simultaneously, geometric morphometrics (Tibihika et al. 2018, Cadrin and Friedland 1999) are used in biodiversity research for association analysis. Those approaches use deterministic two-dimensional locations on digital images (landmarks; Mitteroecker et al. 2013), where each position corresponds to biologically relevant regions of interest. Since this methodology is based on scientific results and compresses image content into deterministic landmarks, no uncertainty regarding those landmark positions is taken into account, which leads to information loss (Pearl 1988). Both, the reduction of this loss and novel knowledge detection, can be done using machine learning. Supervised learning methods (e.g., neuronal nets or support vector machines (Ch. 5 and 6. in Bishop 2006)) map data on prior information (e.g. labels). This increases the performance of classification or regression but affects the latent representation of the data itself. Unsupervised learning (e.g. latent variable models) uses assumptions concerning data structures to extract latent representations without prior information. Those representations does not have to be useful for data processing such as classification and due to that, the use of supervised and unsupervised machine learning and combinations of both, needs to be chosen carefully, according to the application and data. In this work, we discuss unsupervised learning algorithms in terms of explainability, performance and theoretical restrictions in context of known deep learning restrictions (Marcus 2018, Szegedy et al. 2014, Su et al. 2017). We analyse extracted features based on multiple image datasets and discuss shortcomings and performance for processing (e.g. reconstruction error or complexity measurement (Pincus 1997)) using the principal component analysis (Wöber et al. 2013), independent component analysis (Stone 2004), deep neuronal nets (auto encoders; Ch. 14 in Goodfellow 2016) and Gaussian process latent variable models (Titsias and Lawrence 2010, Lawrence 2005).
    Type of Medium: Online Resource
    ISSN: 2535-0897
    Language: Unknown
    Publisher: Pensoft Publishers
    Publication Date: 2019
    detail.hit.zdb_id: 3028709-1
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