In:
PLOS ONE, Public Library of Science (PLoS), Vol. 17, No. 9 ( 2022-9-21), p. e0274956-
Abstract:
Xenorhabdus and Photorhabdus can produce a variety of secondary metabolites with broad spectrum bioactivity against microorganisms. We investigated the antibacterial activity of Xenorhabdus and Photorhabdus against 15 antibiotic-resistant bacteria strains. Photorhabdus extracts had strong inhibitory the growth of Methicillin-resistant Staphylococcus aureus (MRSA) by disk diffusion. The P . akhurstii s subsp. akhurstii (bNN168.5_TH) extract showed lower minimum inhibitory concentrations (MIC) and minimal bactericidal concentrations (MBC). The interaction between either P . akhurstii subsp. akhurstii (bNN141.3_TH) or P . akhurstii subsp. akhurstii (bNN168.5_TH) or P . hainanensis (bNN163.3_TH) extract in combination with oxacillin determined by checkerboard assay exhibited partially synergistic interaction with fractional inhibitory concentration index (FICI) of 0.53. Time-killing assay for P . akhurstii subsp. akhurstii (bNN168.5_TH) extract against S . aureus strain PB36 significantly decreased cell viability from 10 5 CFU/ml to 10 3 CFU/ml within 30 min (P 〈 0.001, t-test). Transmission electron microscopic investigation elucidated that the bNN168.5_TH extract caused treated S . aureus strain PB36 (MRSA) cell membrane damage. The biosynthetic gene clusters of the bNN168.5_TH contained non-ribosomal peptide synthetase cluster (NRPS), hybrid NRPS-type l polyketide synthase (PKS) and siderophore, which identified potentially interesting bioactive products: xenematide, luminmide, xenortide A-D, luminmycin A, putrebactin/avaroferrin and rhizomide A-C. This study demonstrates that bNN168.5_TH showed antibacterial activity by disrupting bacterial cytoplasmic membrane and the draft genome provided insights into the classes of bioactive products. This also provides a potential approach in developing a novel antibacterial agent.
Type of Medium:
Online Resource
ISSN:
1932-6203
DOI:
10.1371/journal.pone.0274956
DOI:
10.1371/journal.pone.0274956.g001
DOI:
10.1371/journal.pone.0274956.g002
DOI:
10.1371/journal.pone.0274956.g003
DOI:
10.1371/journal.pone.0274956.g004
DOI:
10.1371/journal.pone.0274956.g005
DOI:
10.1371/journal.pone.0274956.g006
DOI:
10.1371/journal.pone.0274956.g007
DOI:
10.1371/journal.pone.0274956.g008
DOI:
10.1371/journal.pone.0274956.t001
DOI:
10.1371/journal.pone.0274956.t002
DOI:
10.1371/journal.pone.0274956.t003
DOI:
10.1371/journal.pone.0274956.t004
DOI:
10.1371/journal.pone.0274956.t005
DOI:
10.1371/journal.pone.0274956.s001
DOI:
10.1371/journal.pone.0274956.s002
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2022
detail.hit.zdb_id:
2267670-3
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