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  • 1
    Online Resource
    Online Resource
    Antibacterial Activity of Bacteriocinogenic Commensal Escherichia coli against Zoonotic Strains Resistant and Sensitive to Antibiotics
    MDPI AG ; 2020
    In:  Antibiotics Vol. 9, No. 7 ( 2020-07-15), p. 411-
    Details
    In: Antibiotics, MDPI AG, Vol. 9, No. 7 ( 2020-07-15), p. 411-
    Abstract: Antibiotic resistance concerns various areas with high consumption of antibiotics, including husbandry. Resistant strains are transmitted to humans from livestock and agricultural products via the food chain and may pose a health risk. The commensal microbiota protects against the invasion of environmental strains by secretion of bacteriocins, among other mechanisms. The present study aims to characterize the bactericidal potential of bacteriocinogenic Escherichia coli from healthy humans against multidrug-resistant and antibiotic-sensitive strains from pigs and cattle. Bacteriocin production was tested by the double-layer plate method, and bacteriocin genes were identified by the PCR method. At least one bacteriocinogenic E. coli was detected in the fecal samples of 55% of tested individuals, adults and children. Among all isolates (n = 210), 37.1% were bacteriocinogenic and contained genes of colicin (Col) Ib, ColE1, microcin (Mcc) H47, ColIa, ColM, MccV, ColK, ColB, and single ColE2 and ColE7. Twenty-five E. coli carrying various sets of bacteriocin genes were further characterized and tested for their activity against zoonotic strains (n = 60). Strains with ColE7 (88%), ColE1-ColIa-ColK-MccH47 (85%), MccH47-MccV (85%), ColE1-ColIa-ColM (82%), ColE1 (75%), ColM (67%), and ColK (65%) were most active against zoonotic strains. Statistically significant differences in activity toward antibiotic-resistant strains were shown by commensal E. coli carrying MccV, ColK-MccV, and ColIb-ColK. The study demonstrates that bacteriocinogenic commensal E. coli exerts antagonistic activity against zoonotic strains and may constitute a defense line against multidrug-resistant strains.
    Type of Medium: Online Resource
    ISSN: 2079-6382
    URL: Article
    DOI: 10.3390/antibiotics9070411
    Language: English
    Publisher: MDPI AG
    Publication Date: 2020
    detail.hit.zdb_id: 2681345-2
    SSG: 15,3
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  • 2
    Online Resource
    Online Resource
    Extended Phylogeny and Extraintestinal Virulence Potential of Commensal Escherichia coli from Piglets and Sows
    MDPI AG ; 2020
    In:  International Journal of Environmental Research and Public Health Vol. 17, No. 1 ( 2020-01-06), p. 366-
    Details
    In: International Journal of Environmental Research and Public Health, MDPI AG, Vol. 17, No. 1 ( 2020-01-06), p. 366-
    Abstract: Commensal Escherichia coli, naturally occurring in the intestinal tract, can be the origin of extraintestinal pathogenic E. coli (ExPEC) strains. ExPEC causes high mortality and significant economic losses in the swine industry in several countries and poses a serious threat to public health worldwide. The aim of this study was to analyze the extended phylogenetic structure and extraintestinal virulence potential in two groups of commensal E. coli isolates from post-weaning piglets and sows. The phylogenetic assignment to eight groups was determined using the revised Clermont phylogenetic typing method in quadruplex PCR. Identification of extraintestinal virulence genes (VGs) and adhesin operon genes was performed using multiplex or simplex PCR. The revised phylogenetic assignment allowed us to distinguish E. coli with significantly higher (groups C and F) or lower (group E) virulence potential in isolates from piglets. The majority of the tested VGs occurred more frequently in isolates from piglets than from sows, with statistically significant differences for seven genes: fimH, papAH, iutA, iroN, ompT, traT, and iss. Complete operons for type I and P fimbriae significantly prevailed among E. coli from piglets. This study provides insight into the extended phylogenetic structure of porcine commensal E. coli and showed that these strains, particularly from piglets, constitute a considerable reservoir of extraintestinal VGs and may increase the potential risk of extraintestinal infections.
    Type of Medium: Online Resource
    ISSN: 1660-4601
    URL: Article
    DOI: 10.3390/ijerph17010366
    Language: English
    Publisher: MDPI AG
    Publication Date: 2020
    detail.hit.zdb_id: 2175195-X
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  • 3
    Online Resource
    Online Resource
    Evaluation of the Antibacterial Activity of Ag- and Au-Nanoparticles Loaded TiO 2 Nanotubes
    American Scientific Publishers ; 2020
    In:  Journal of Biomedical Nanotechnology Vol. 16, No. 9 ( 2020-09-01), p. 1416-1425
    Details
    In: Journal of Biomedical Nanotechnology, American Scientific Publishers, Vol. 16, No. 9 ( 2020-09-01), p. 1416-1425
    Abstract: Current research on the antibacterial properties of implant surfaces has focused on using titanium nanotubes (TNTs) with diameters of 100 and 200 nm, which simultaneously show the best antibacterial properties, poor osseointegration, and ability to immobilize proteins. Therefore, the research aimed to develop an implantable material based on titanium dioxide nanotubes with a diameter of 50 nm doped with silver (AgNPs) and gold nanoparticles (AuNPs), indicating good absorption and antibacterial properties. Moreover, metallic nanoparticles deposited by varying methods should maintain sphericity and lack of agglomeration. For this purpose, the surface charge, wettability, stability of nanoparticles, and antibacterial properties against Gram-positive and Gram-negative bacteria, i. e., Staphylococcus epidermidis, Streptococcus mutans, and Pseudomonas aeruginosa , were performed. Obtained results indicate a greater resistance to leaching of silver nanoparticles compared to gold nanoparticles. These results are reflected in microbiological studies, both into the time and the effectiveness of the implantable material's antibacterial activity. A greater antibacterial effect of AgNPs than AuNPs has been confirmed. Also, AgNPs inhibit the multiplication of Gram-negative bacteria to a greater extent than Gram-positive bacteria. It has been proven that the TNT platforms deposited with metal nanoparticles via the voltammetric method are more effective in deactivating microorganisms. Besides, the results have proven that smaller TNTs effectively reduce live bacteria as nanotubes with a diameter of 100 and 200 nm.
    Type of Medium: Online Resource
    ISSN: 1550-7033
    URL: Article
    DOI: 10.1166/jbn.2020.2979
    Language: English
    Publisher: American Scientific Publishers
    Publication Date: 2020
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  • 4
    Online Resource
    Online Resource
    Evaluation of biofilm formation on acrylic resins used to fabricate dental temporary restorations with the use of 3D printing technology
    Springer Science and Business Media LLC ; 2022
    In:  BMC Oral Health Vol. 22, No. 1 ( 2022-10-13)
    Details
    In: BMC Oral Health, Springer Science and Business Media LLC, Vol. 22, No. 1 ( 2022-10-13)
    Abstract: Temporary implant-retained restorations are required to support function and esthetics of the masticatory system until the final restoration is completed and delivered. Acrylic resins are commonly used in prosthetic dentistry and lately they have been used in three-dimensional (3D) printing technology. Since this technology it is fairly new, the number of studies on their susceptibility to microbial adhesion is low. Restorations placed even for a short period of time may become the reservoir for microorganisms that may affect the peri-implant tissues and trigger inflammation endangering further procedures. The aim of the study was to test the biofilm formation on acrylamide resins used to fabricate temporary restorations in 3D printing technology and to assess if the post-processing impacts microbial adhesion. Methods Disk-shaped samples were manufactured using the 3D printing technique from three commercially available UV-curable resins consisting of acrylate and methacrylate oligomers with various time and inhibitors of polymerization (NextDent MFH bleach, NextDent 3D Plus, MazicD Temp). The tested samples were raw, polished and glazed. The ability to create biofilm by oral streptococci ( S. mutans , S. sanguinis, S. oralis, S. mitis ) was tested, as well as species with higher pathogenic potential: Staphylococcus aureus, Staphylococcus epidermidis and Candida albicans . The roughness of the materials was measured by an atomic force microscope. Biofilm formation was assessed after 72 h of incubation by crystal violet staining with absorbance measurement, quantification of viable microorganisms, and imaging with a scanning electron microscope (SEM). Results Each tested species formed the biofilm on the samples of all three resins. Post-production processing resulted in reduced roughness parameters and biofilm abundance. Polishing and glazing reduced roughness parameters significantly in the NextDent resin group, while glazing alone caused significant surface smoothing in Mazic Temp. A thin layer of microbial biofilm covered glazed resin surfaces with a small number of microorganisms for all tested strains except S. oralis and S. epidermidis, while raw and polished surfaces were covered with a dense biofilm, rich in microorganisms. Conclusions UV-curing acrylic resins used for fabricating temporary restorations in the 3D technology are the interim solution, but are susceptible to adhesion and biofilm formation by oral streptococci , staphylococci and Candida . Post-processing and particularly glazing process significantly reduce bacterial biofilm formation and the risk of failure of final restoration.
    Type of Medium: Online Resource
    ISSN: 1472-6831
    URL: Article
    DOI: 10.1186/s12903-022-02488-5
    Language: English
    Publisher: Springer Science and Business Media LLC
    Publication Date: 2022
    detail.hit.zdb_id: 2091511-1
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  • 5
    Online Resource
    Online Resource
    Susceptibility to biofilm formation on 3D-printed titanium fixation plates used in the mandible: a preliminary study
    Informa UK Limited ; 2020
    In:  Journal of Oral Microbiology Vol. 12, No. 1 ( 2020-01-01), p. 1838164-
    Details
    In: Journal of Oral Microbiology, Informa UK Limited, Vol. 12, No. 1 ( 2020-01-01), p. 1838164-
    Type of Medium: Online Resource
    ISSN: 2000-2297
    URL: Article
    DOI: 10.1080/20002297.2020.1838164
    Language: English
    Publisher: Informa UK Limited
    Publication Date: 2020
    detail.hit.zdb_id: 2523919-3
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  • 6
    Online Resource
    Online Resource
    Changes in the Mechanical Properties of Alginate-Gelatin Hydrogels with the Addition of Pygeum africanum with Potential Application in Urology
    MDPI AG ; 2022
    In:  International Journal of Molecular Sciences Vol. 23, No. 18 ( 2022-09-07), p. 10324-
    Details
    In: International Journal of Molecular Sciences, MDPI AG, Vol. 23, No. 18 ( 2022-09-07), p. 10324-
    Abstract: New hydrogel materials developed to improve soft tissue healing are an alternative for medical applications, such as tissue regeneration or enhancing the biotolerance effect in the tissue-implant–body fluid system. The biggest advantages of hydrogel materials are the presence of a large amount of water and a polymeric structure that corresponds to the extracellular matrix, which allows to create healing conditions similar to physiological ones. The present work deals with the change in mechanical properties of sodium alginate mixed with gelatin containing Pygeum africanum. The work primarily concentrates on the evaluation of the mechanical properties of the hydrogel materials produced by the sol–gel method. The antimicrobial activity of the hydrogels was investigated based on the population growth dynamics of Escherichia coli ATCC 25922 and Staphylococcus aureus ATCC 25923, as well as the degree of degradation after contact with urine using an innovative method with a urine flow simulation stand. On the basis of mechanical tests, it was found that sodium alginate-based hydrogels with gelatin showed weaker mechanical properties than without the additive. In addition, gelatin accelerates the degradation process of the produced hydrogel materials. Antimicrobial studies have shown that the presence of African plum bark extract in the hydrogel enhances the inhibitory effect on Gram-positive and Gram-negative bacteria. The research topic was considered due to the increased demand from patients for medical devices to promote healing of urethral epithelial injuries in order to prevent the formation of urethral strictures.
    Type of Medium: Online Resource
    ISSN: 1422-0067
    URL: Article
    DOI: 10.3390/ijms231810324
    Language: English
    Publisher: MDPI AG
    Publication Date: 2022
    detail.hit.zdb_id: 2019364-6
    SSG: 12
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  • 7
    Online Resource
    Online Resource
    Personalized, 3D- printed fracture fixation plates versus commonly used orthopedic implant materials- biomaterials characteristics and bacterial biofilm formation
    Elsevier BV ; 2022
    In:  Injury Vol. 53, No. 3 ( 2022-03), p. 938-946
    Details
    In: Injury, Elsevier BV, Vol. 53, No. 3 ( 2022-03), p. 938-946
    Type of Medium: Online Resource
    ISSN: 0020-1383
    URL: Article
    DOI: 10.1016/j.injury.2021.12.020
    Language: English
    Publisher: Elsevier BV
    Publication Date: 2022
    detail.hit.zdb_id: 2011808-9
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