In:
British Journal of Surgery, Oxford University Press (OUP), Vol. 85, No. 3 ( 2003-01-22), p. 337-340
Abstract:
In patients suffering from chronic pancreatitis the risk for the development of pancreatic cancer ranges from 4 to 6 per cent. Various mutations are associated with pancreatic cancer, especially of p53 and K-ras. The incidence of these mutations in resected chronic pancreatitic tissue was investigated. Methods In the present study DNA from 80 samples of tissue from patients with chronic pancreatitis was isolated and subjected to single-strand conformation polymorphism (SSCP) analysis of p53 exons 5–9 and restriction fragment length polymorphism analysis of K-ras (codon 12). Results No mutations in the K-ras gene were detected. On SSCP analysis, eight of 80 cases of chronic pancreatitis showed alterations (two in exon 5, four in exon 6, two in exon 7). DNA sequence analysis revealed one deletion of 21 amino acids (exon 5), four polymorphisms in exon 6 with no change in the amino acid sequence, one point mutation in exon 5, and two point mutations located in the intron between exons 6 and 7. Conclusion These data show that in some cases of chronic pancreatitis mutations in the p53 gene occur without morphological evidence of pancreatic cancer.
Type of Medium:
Online Resource
ISSN:
0007-1323
,
1365-2168
DOI:
10.1046/j.1365-2168.1998.00630.x
Language:
English
Publisher:
Oxford University Press (OUP)
Publication Date:
2003
detail.hit.zdb_id:
2006309-X
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