In:
International Journal of Peptide and Protein Research, Wiley, Vol. 47, No. 1-2 ( 1996-01), p. 70-83
Abstract:
We have developed a new resin for peptide synthesis that can be used to synthesize and evaluate directly combinatorial peptide libraries for binding target proteins. Fidelity of the peptide synthesis using this hydrophilic resin is comparable to polystyrene‐based resins. Peptide libraries synthesized on this resin were probed by a two color PEptide Library Immunostaining Chromatographic ANalysis (PELICAN) technique for sequences binding the serine protease Factor IX zynogen. This PELICAN technique readily distinguishes between beads interacting with the reagents for target detection (blue beads) from those beads specific for the target protein itself (red beads). Validation of the PELICAN technique, as well as purification of Factor IX from plasma, is demonstrated utilizing this resin. ©Munksgaard 1996.
Type of Medium:
Online Resource
ISSN:
0367-8377
DOI:
10.1111/jpp.1996.47.issue-1-2
DOI:
10.1111/j.1399-3011.1996.tb00812.x
Language:
English
Publisher:
Wiley
Publication Date:
1996
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