In:
PLOS ONE, Public Library of Science (PLoS), Vol. 16, No. 10 ( 2021-10-27), p. e0259114-
Abstract:
Systemic lupus erythematosus (SLE) is an autoimmune disease characterized by the production of autoantibodies such as anti-Sm. Studies in patients with SLE and murine models of lupus reveal that the most critical anti-Sm autoantibodies are predominantly direct against D1 (83–119), D2, and B´/B epitopes. Objectives The present study aimed to analyze the induction of antigen-specific tolerance after prophylactic immunization with a DNA vaccine encoding the epitopes: D1 83-119 , D2, B´/B, and B´/B COOH in co-vaccination with IFN-γ or IL-10 in a murine model of lupus induced by pristane. Material and methods To obtain endotoxin-free DNA vaccines, direct cloning techniques using pcDNA were performed: D1 83-119 , D2, B´/B, B´/B COOH , IFN-γ, or IL-10. Lupus was induced by 0.5 mL of pristane via intraperitoneal in BALB/c female mice. Immunoprecipitation with K562 cells was metabolically labeled with 35 S and ELISA to detect serum antibodies or mice IgG1, IgG2a isotypes. ELISA determined IL-10 and IFN-γ from splenocytes supernatants. Proteinuria was assessed monthly, and lupus nephritis was evaluated by immunofluorescence, and electron microscopy. Results The prophylactic co-vaccination with D2/IL-10 reduced the expression of kidney damage observed by electron microscopy, direct immunofluorescence, and H & E, along with reduced level of anti-nRNP/Sm antibodies ( P = 0.048). Conclusion The prophylactic co-vaccination of IL-10 with D2 in pristane-induced lupus ameliorates the renal damage maybe by acting as prophylactic DNA tolerizing therapy.
Type of Medium:
Online Resource
ISSN:
1932-6203
DOI:
10.1371/journal.pone.0259114
DOI:
10.1371/journal.pone.0259114.g001
DOI:
10.1371/journal.pone.0259114.g002
DOI:
10.1371/journal.pone.0259114.g003
DOI:
10.1371/journal.pone.0259114.g004
DOI:
10.1371/journal.pone.0259114.g005
DOI:
10.1371/journal.pone.0259114.g006
DOI:
10.1371/journal.pone.0259114.g007
DOI:
10.1371/journal.pone.0259114.s001
DOI:
10.1371/journal.pone.0259114.s002
DOI:
10.1371/journal.pone.0259114.s003
DOI:
10.1371/journal.pone.0259114.s004
DOI:
10.1371/journal.pone.0259114.r001
DOI:
10.1371/journal.pone.0259114.r002
DOI:
10.1371/journal.pone.0259114.r003
DOI:
10.1371/journal.pone.0259114.r004
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2021
detail.hit.zdb_id:
2267670-3
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