In:
PLOS ONE, Public Library of Science (PLoS), Vol. 16, No. 6 ( 2021-6-21), p. e0253494-
Abstract:
The sla1 + gene of Schizosachharoymces pombe encodes La protein which promotes proper processing of precursor-tRNAs. Deletion of sla1 ( sla1 Δ) leads to disrupted tRNA processing and sensitivity to target of rapamycin (TOR) inhibition. Consistent with this, media containing NH4 + inhibits leucine uptake and growth of sla1 Δ cells. Here, transcriptome analysis reveals that genes upregulated in sla1 Δ cells exhibit highly significant overalp with general amino acid control (GAAC) genes in relevant transcriptomes from other studies. Growth in NH4 + media leads to additional induced genes that are part of a core environmental stress response (CESR). The sla1 Δ GAAC response adds to evidence linking tRNA homeostasis and broad signaling in S . pombe . We provide evidence that deletion of the Rrp6 subunit of the nuclear exosome selectively dampens a subset of GAAC genes in sla1 Δ cells suggesting that nuclear surveillance-mediated signaling occurs in S . pombe . To study the NH4 + -effects, we isolated sla1 Δ spontaneous revertants (SSR) of the slow growth phenotype and found that GAAC gene expression and rapamycin hypersensitivity were also reversed. Genome sequencing identified a F32V substitution in Any1, a known negative regulator of NH4 + -sensitive leucine uptake linked to TOR. We show that 3 H-leucine uptake by SSR- any1-F32V cells in NH4 + -media is more robust than by sla1 Δ cells. Moreover, F32V may alter any1 + function in sla1 Δ vs. sla1 + cells in a distinctive way. Thus deletion of La, a tRNA processing factor leads to a GAAC response involving reprogramming of amino acid metabolism, and isolation of the any1-F32V rescuing mutant provides an additional specific link.
Type of Medium:
Online Resource
ISSN:
1932-6203
DOI:
10.1371/journal.pone.0253494
DOI:
10.1371/journal.pone.0253494.g001
DOI:
10.1371/journal.pone.0253494.g002
DOI:
10.1371/journal.pone.0253494.g003
DOI:
10.1371/journal.pone.0253494.g004
DOI:
10.1371/journal.pone.0253494.g005
DOI:
10.1371/journal.pone.0253494.g006
DOI:
10.1371/journal.pone.0253494.t001
DOI:
10.1371/journal.pone.0253494.s001
DOI:
10.1371/journal.pone.0253494.s002
DOI:
10.1371/journal.pone.0253494.s003
DOI:
10.1371/journal.pone.0253494.s004
DOI:
10.1371/journal.pone.0253494.s005
DOI:
10.1371/journal.pone.0253494.s006
DOI:
10.1371/journal.pone.0253494.s007
DOI:
10.1371/journal.pone.0253494.s008
DOI:
10.1371/journal.pone.0253494.s009
DOI:
10.1371/journal.pone.0253494.s010
DOI:
10.1371/journal.pone.0253494.r001
DOI:
10.1371/journal.pone.0253494.r002
DOI:
10.1371/journal.pone.0253494.r003
DOI:
10.1371/journal.pone.0253494.r004
DOI:
10.1371/journal.pone.0253494.r005
DOI:
10.1371/journal.pone.0253494.r006
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2021
detail.hit.zdb_id:
2267670-3
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