In:
Annals of Clinical Biochemistry: International Journal of Laboratory Medicine, SAGE Publications, Vol. 38, No. 5 ( 2001-09), p. 554-558
Abstract:
Accurate measurement of pyruvate is particularly important in the detection of mitochondrial enzyme disorders. We describe a simple high-performance liquid chromatography assay for pyruvate with derivatization and fluorescence detection. Between-run variation is below 4·5% and the method is linear between 1 and 1200μmol/L. The mean recovery is 101%. After sample pretreatment, the calculated pyruvate concentration proved to be stable for 15 days when samples were kept at −20°C.
Type of Medium:
Online Resource
ISSN:
0004-5632
,
1758-1001
DOI:
10.1177/000456320103800514
Language:
English
Publisher:
SAGE Publications
Publication Date:
2001
detail.hit.zdb_id:
2041298-8
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