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  • 1
    Online Resource
    Online Resource
    Investigation of culture conditions for recombinant xylanase a production and its enzymatic hydrolysis of agricultural wastes
    Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications) ; 2023
    In:  Vietnam Journal of Biotechnology Vol. 21, No. 1 ( 2023-08-26), p. 179-187
    Details
    In: Vietnam Journal of Biotechnology, Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications), Vol. 21, No. 1 ( 2023-08-26), p. 179-187
    Abstract: Xylanase A of Aspergillus niger dsm 1957 was successfully expressed in strain Pichia pastoris GS115/pXlnA in YP medium induced by methanol. Molecular weight of the recombinant xylanase A was 35 kDa, that was consistent with the theoretical calculation and the enzyme activity in the culture was 7310 U/mL. Maximal xylanase activity (11180 U/mL) was gained after culturing the recombinant yeast for 120 hours in the present of 1% methanol. Among of seven media (BMMY, MMY, MM, YPM, YPTM, YPTCM, and YP) utilized for the yeast culture, the highest activity of the produced recombinant xylanase A (21620 U/mL) was reached in BMMY medium, while the lowest activity (1410 U/mL) was found in YPTCM medium. At the appropriate conditions, the recombinant xylanase A activity was 2.96 folds higher than that expressed in normal conditions. The conditions for recombinant xylanase A enzymatic hydrolysis of several agricultural wastes were also investigated. The results showed that in appropriate conditions (40oC, 24 hours, substrate concentration of 40 mg), the highest amount of reducing sugars produced from cob, rice bran and soybean meal substrates were 0.617 ± 0.002 μmol/mL, 0.663 ± 0.002 μmol/mL, and 0.814 ± 0.003 μmol/mL, respectively. Overall, with these distinctive properties, the recombinant xylanase A may initially become a potential candidate for various industrial applications.
    Type of Medium: Online Resource
    ISSN: 2815-5912 , 2815-5955
    URL: Article
    DOI: 10.15625/1811-4989/17659
    Language: Unknown
    Publisher: Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications)
    Publication Date: 2023
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  • 2
    Online Resource
    Online Resource
    Phát hiện một số vi rút gây bệnh ở người trong môi trường nước thải tại Hà Nội, năm 2020 – 2021
    Tap chi Y hoc du phong (Vietnam Journal of Preventive Medicine-VJPM) ; 2022
    In:  Tạp chí Y học Dự phòng Vol. 32, No. 7 ( 2022-11-18), p. 30-39
    Details
    In: Tạp chí Y học Dự phòng, Tap chi Y hoc du phong (Vietnam Journal of Preventive Medicine-VJPM), Vol. 32, No. 7 ( 2022-11-18), p. 30-39
    Abstract: Giám sát tác nhân vi rút gây bệnh ở người trong nước thải là mô hình giám sát sức khỏe cộng đồng chủ động được nhiều quốc gia áp dụng. Tại Việt Nam, chưa có công bố nào về giám sát các vi rút gây bệnh trong nước thải. Dựa vào chương trình giám sát vi rút polio trong nước thải được triển khai tại Hà Nội từ tháng 11/2020, nghiên cứu này nhằm mục tiêu xác định sự có mặt của một số vi rút gây bệnh khác. 53 mẫu nước thải chưa qua xử lý được thu thập từ hai trạm xử lý và bốn cống gom nước thải sinh hoạt ở các sông tại Hà Nội, giai đoạn 11/2020 – 12/2021. Mẫu nước được cô đặc theo phương pháp 2-pha Dextran PEG và sau đó xét nghiệm phát hiện vi rút đường ruột (VRĐR), rota, noro và adeno. VRĐR phân lập được trên 6 (11,32%) mẫu, bao gồm echo 3, 6 và 11, mỗi type 2 mẫu. Vi rút rota, noro GI, noro GII và adeno lần lượt phát hiện trên 24 (45,28%), 16 (30,18%), 27 (50,94%) và 33 (62,26%) mẫu. Kết quả bước đầu cho thấy tính khả thi trong việc kết hợp song song giám sát vi rút polio với giám sát các vi rút gây bệnh khác trong môi trường nước thải, đặc biệt đối với vi rút noro và adeno mà hệ thống giám sát dựa trên bệnh nhân chưa được thực hiện tại Việt Nam.
    Type of Medium: Online Resource
    ISSN: 0868-2836
    URL: Article
    DOI: 10.51403/0868-2836/2022/839
    Language: Unknown
    Publisher: Tap chi Y hoc du phong (Vietnam Journal of Preventive Medicine-VJPM)
    Publication Date: 2022
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  • 3
    Online Resource
    Online Resource
    Selection, purification, and evaluation of acarbose−an α-glucosidase inhibitor from Actinoplanes sp.
    Elsevier BV ; 2021
    In:  Chemosphere Vol. 265 ( 2021-02), p. 129167-
    Details
    In: Chemosphere, Elsevier BV, Vol. 265 ( 2021-02), p. 129167-
    Type of Medium: Online Resource
    ISSN: 0045-6535
    URL: Article
    DOI: 10.1016/j.chemosphere.2020.129167
    RVK:
    AR 10100
    Language: English
    Publisher: Elsevier BV
    Publication Date: 2021
    detail.hit.zdb_id: 1496851-4
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  • 4
    Online Resource
    Online Resource
    Autologous bone marrow mononuclear cell infusion for liver cirrhosis after the Kasai operation in children with biliary atresia
    Springer Science and Business Media LLC ; 2022
    In:  Stem Cell Research & Therapy Vol. 13, No. 1 ( 2022-12)
    Details
    In: Stem Cell Research & Therapy, Springer Science and Business Media LLC, Vol. 13, No. 1 ( 2022-12)
    Abstract: To evaluate the safety and early outcomes of autologous bone marrow mononuclear cell (BMMNC) infusion for liver cirrhosis due to biliary atresia (BA) after Kasai operation. Methods An open-label clinical trial was performed from January 2017 to December 2019. Nineteen children with liver cirrhosis due to BA after Kasai operation were included. Bone marrow was harvested through anterior iliac crest puncture under general anesthesia. Mononuclear cells (MNCs) were isolated by Ficoll gradient centrifugation and then infused into the hepatic artery. The same procedure was repeated 6 months later. Serum bilirubin, albumin, alanine aminotransferase, aspartate aminotransferase, gamma-glutamyl transferase, and prothrombin time were monitored at baseline, 3 months, 6 months, and 12 months after the first transplantation. Esophagoscopies and liver biopsies were performed in patients whose parents provided consent. Mixed-effect analysis was used to evaluate the changes in Pediatric End-Stage Liver Disease (PELD) scores. Results The average MNC and CD34+ cell counts per kg body weight were 50.1 ± 58.5 × 10 6 /kg and 3.5 ± 2.8 × 10 6 for the first transplantation and 57.1 ± 42.0 × 10 6 /kg and 3.7 ± 2.7 × 10 6 for the second transplantation. No severe adverse events associated with the cell therapy were observed in the patients. One patient died 5 months after the first infusion at a provincial hospital due to the rupture of esophageal varices, while 18 patients survived. Liver function was maintained or improved after infusion, as assessed by biochemical tests. The severity of the disease reduced markedly, with a significant reduction in PELD scores. Conclusion Autologous BMMNC administration for liver cirrhosis due to BA is safe and may maintain or improve liver function. Trial registration ClinicalTrials.gov identifier: NCT03468699. Name of the registry: Vinmec Research Institute of Stem Cell and Gene Technology. https://clinicaltrials.gov/ct2/show/NCT03468699?cond=biliary+atresia & cntry=VN & draw=2 & rank=2 . Registered on March 16, 2018. The trial results will also be published according to the CONSORT statement at conferences and reported in peer-reviewed journals.
    Type of Medium: Online Resource
    ISSN: 1757-6512
    URL: Article
    DOI: 10.1186/s13287-022-02762-x
    Language: English
    Publisher: Springer Science and Business Media LLC
    Publication Date: 2022
    detail.hit.zdb_id: 2548671-8
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  • 5
    Online Resource
    Online Resource
    Cloning, Expression, and Characterization of Xylanase G2 from Aspergillus oryzae VTCC-F187 in Aspergillus niger VTCC-F017
    Hindawi Limited ; 2021
    In:  BioMed Research International Vol. 2021 ( 2021-3-11), p. 1-9
    Details
    In: BioMed Research International, Hindawi Limited, Vol. 2021 ( 2021-3-11), p. 1-9
    Abstract: The study focuses on engineering of recombinant Aspergillus niger to produce highly active xylanase. The xylanase G2 encoding gene originating from Aspergillus oryzae VTCC-F187 was cloned, amplified, and inserted into the pAN7.1GluA vector with specific primers possessing BamHI. The recombinant plasmid was introduced into Aspergillus niger VTCC-F017 by chemical methods. The recombinant strain was checked by polymerase chain reaction method and Southern blot. Next, the recombinant protein was expressed and purified by His-tag column. The molecular mass of the purified xylanase G2, as determined by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE), was 21 kDa with a specific activity of 1025 IU/mg towards 0.5% (w/v) of birchwood xylan. The optimal temperature and pH were 55°C and pH 6.5, respectively. The enzyme was stable in a temperature ranges 25–40°C and a pH ranges 5–7. The presence of Tween 80 enhanced xylanase activity. Triton X-100, however, had no impact on the function of the enzyme. The xylanase activity was reduced by Tween 20, SDS, and organic solvents. The enzyme was completely inhibited by Hg2+ and partially by Zn2+, Fe2+, and Ag+, while it was slightly stimulated by K+ and EDTA.
    Type of Medium: Online Resource
    ISSN: 2314-6141 , 2314-6133
    URL: Article
    DOI: 10.1155/2021/8840038
    Language: English
    Publisher: Hindawi Limited
    Publication Date: 2021
    detail.hit.zdb_id: 2698540-8
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  • 6
    Online Resource
    Online Resource
    Elucidation and Identification of an Antifungal Compound from Pseudomonas aeruginosa DA3.1 Isolated from Soil in Vietnam
    Briefland ; 2020
    In:  Jundishapur Journal of Microbiology Vol. 13, No. 10 ( 2020-12-05)
    Details
    In: Jundishapur Journal of Microbiology, Briefland, Vol. 13, No. 10 ( 2020-12-05)
    Abstract: Background: Fusarium sp. and Rhizoctonia sp. fungi have been always threats to short-term crops. In Vietnam, corn and soybean suffer serious losses annually. Therefore, it is necessary to utilize an environmentally friendly antifungal compound that is highly effective against phytopathogenic fungi. Pseudomonas sp. is a popular soil bacterial strain and well known for its high antifungal activity. Objectives: This study was carried out to evaluate and assess the antifungal activity of a local bacterial strain namely DA3.1 that was later identified as Pseudomonas aeruginosa. This would be strong scientific evidence to develop an environmentally friendly biocide from a local microorganism strain for commercial use. Methods: The antifungal compound was purified from ethyl acetate extraction of deproteinized cell culture broth by a silica gel column (CH2Cl2/MeOH (0% - 10% MeOH)). The purity of the isolated compound was determined by HPLC, and its molecular structure was elucidated using spectroscopic experiments including one-dimensional (1D) (1H NMR, 13C NMR, DEPT) and two-dimensional (2D) (HMBC and HSQC) spectra. The activity of the purified compound against Fusarium sp. and Rhizoctonia sp. fungi was measured using the PDA-disk diffusion method, and its growth-promoting ability was evaluated using the seed germination test of corn and soybean. Results: The results showed that the antifungal compound produced by Pseudomonas aeruginosa DA3.1 had a retention factor (Rf) of 0.86 on thin layer chromatography (TLC). Based on the evidence of spectral data including proton nuclear magnetic resonance (1H NMR), carbon nuclear magnetic resonance (13C NMR), distortionless enhancement by polarization transfer (DEPT), heteronuclear multiple bond correlation (HMBC), and heteronuclear single quantum coherence (HSQC), the chemical structure was elucidated as phenazine-1-carboxylic. The purified compound showed inhibitory activity against F. oxysporum and R. solani and exhibited the ability of the germination of corn and soybean seeds. The results revealed the benefit of native P. aeruginosa DA3.1 and phenazine-1-carboxylic acid for use as a biocontrol agent, as well as a plant growth promoter. Conclusions: The antifungal compound isolated from local Pseudomonas DA3.1 was identified as phenazine-1-carboxylic acid that posed high antifungal activity and was a plant germination booster.
    Type of Medium: Online Resource
    ISSN: 2008-3645 , 2008-4161
    URL: Journal
    URL: Article
    DOI: 10.5812/jjm
    DOI: 10.5812/jjm.103792
    Language: Unknown
    Publisher: Briefland
    Publication Date: 2020
    detail.hit.zdb_id: 2815501-4
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  • 7
    Online Resource
    Online Resource
    Protection and Rehabilitation Effects of Cordyceps militaris Fruit Body Extract and Possible Roles of Cordycepin and Adenosine
    MDPI AG ; 2022
    In:  Compounds Vol. 2, No. 4 ( 2022-12-19), p. 388-403
    Details
    In: Compounds, MDPI AG, Vol. 2, No. 4 ( 2022-12-19), p. 388-403
    Abstract: Cordyceps militaris is a valued medicinal fungus in folk medicine in East Asia. It contains two major nucleosides, cordycepin and adenosine, which have been reported to have potential antineoplastic, antioxidant, and anti-inflammatory activities. This paper aimed to study the effect of C. militaris extract on the reproductive function of a mouse model, evaluating possible toxicity, androgenic activity, and protective and rehabilitative effects against damages caused by sodium valproate (VPA). There was no death and abnormalities observed in mice. Androgen activity was also shown in young male rats by an improvement in several sexual organs. The protective effect of C. militaris extract was explained by the gain of sexual organs’ weight, testosterone concentration, and seminiferous tubule size as well as the enhancement of sperm density, alive sperm percentage, and the progressive forward movement of sperm. The pregnancy rate of female rats paired with VPA-administered male rats (500 mg/kg/day) increased proportionally with the higher dose of C. militaris extract. In the rehabilitation study, an incline in the weight of the Cowper’s gland and glans (0.112 g/kg/day) and testicle and prostate (0.336 g/kg/day) as well as an improvement of the sperm forward progressive movement was observed. The percentage of unprogressive sperm and immotile sperm has reduced. These results suggest that C. militaris is a potential supplement to reduce the negative effects of VPA and improve reproductive function, in which the two major constituents cordycepin and adenosine may play an active role.
    Type of Medium: Online Resource
    ISSN: 2673-6918
    URL: Article
    DOI: 10.3390/compounds2040032
    Language: English
    Publisher: MDPI AG
    Publication Date: 2022
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  • 8
    Online Resource
    Online Resource
    Purification and characterization of an antifungal protein from Bacillus subtilis XL62 isolated in Vietnam
    Science Society of Thailand ; 2017
    In:  ScienceAsia Vol. 43, No. 5 ( 2017), p. 294-
    Details
    In: ScienceAsia, Science Society of Thailand, Vol. 43, No. 5 ( 2017), p. 294-
    Type of Medium: Online Resource
    ISSN: 1513-1874
    URL: Article
    DOI: 10.2306/scienceasia1513-1874.2017.43.294
    Language: English
    Publisher: Science Society of Thailand
    Publication Date: 2017
    detail.hit.zdb_id: 2590152-7
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  • 9
    Online Resource
    Online Resource
    Purification of wild type and recombinant xylanases and evaluation of their arabinoxylan hydrolysis ability
    Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications) ; 2022
    In:  Vietnam Journal of Biotechnology Vol. 19, No. 4 ( 2022-05-03), p. 741-748
    Details
    In: Vietnam Journal of Biotechnology, Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications), Vol. 19, No. 4 ( 2022-05-03), p. 741-748
    Abstract: Arabinoxylan hydrolyzing enzymes are very rich and diversified groups, among these, endo-1,4-β-xylanase is the most important group which catalyzes the cleavage of xylan main skeleton to release arabinoxylan oligosaccharides such as D-xylose, xylobiose, L-arabinose, xylotetraose, xylopentose.  This group of enzyme was efficiently produced from natural microorganism including bacteria, yeast and fungi and recombinant strains. In this study, we have purified wild-type and recombinant xylanase to compare the difference in hydrolysis products of these two enzymes. Using sephadex G-100 and DEAE-sephadex column chromatography, we have purified xylanase from wild-type Aspergillus niger DSM1957 with molecular weight of 25 kDa, specific activity of 3240 IU/mL, the purity increases 1.68 folds compare to crude extract, and a recovery rate of 36%. The recombinant enzyme from Pichia pastoris GS115/pPXlnA was purified by ProbondTM affinity chromatography column, with a molecular weight of 36 kDa; the purity increased 3.2 folds than the crude extract, and with the recovery efficiency of 21.1%. The activity of commercial wild-type xylanase was better than that of our recombinant and wild-type enzymes. Our wild-type xylanase could hydrolyze arabinoxylan into L-arabinose in 50 mM CH3COONa pH 5.0. Our results showed that xylanases purified from different sources were stable and highly specific to xylan. Xylanase has potential application in the production of high quality biotechnological products.
    Type of Medium: Online Resource
    ISSN: 1811-4989 , 1811-4989
    URL: Article
    DOI: 10.15625/1811-4989/15251
    Language: Unknown
    Publisher: Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications)
    Publication Date: 2022
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  • 10
    Online Resource
    Online Resource
    Extracellular polymeric substances production in sludge by bacteria isolated from Hanoi brewery
    Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications) ; 2015
    In:  TAP CHI SINH HOC Vol. 36, No. 3 ( 2015-03-26)
    Details
    In: TAP CHI SINH HOC, Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications), Vol. 36, No. 3 ( 2015-03-26)
    Type of Medium: Online Resource
    ISSN: 0866-7160 , 0866-7160
    URL: Article
    DOI: 10.15625/0866-7160/v36n3.5996
    Language: Unknown
    Publisher: Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications)
    Publication Date: 2015
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