In:
The Journal of Immunology, The American Association of Immunologists, Vol. 182, No. 1_Supplement ( 2009-04-01), p. 40.20-40.20
Abstract:
Natural killer (NK) cells play an important role in elimination of altered self cell, such as tumor cells. Recently, vitamin C is an essential component for augmentation of NK cell activity, especially in cancer patients. In this study, the effects of vitamin C on the anti-tumor activity of NKDC (CD49b+CD11c+NK1.1+) were investigated in Gulo(-/-) mice, which can not biosynthesize vitamin C like human beings. Body weight and the size of spleen of vitamin C depleted Gulo(-/-) mice was significantly decreased at 2 weeks after vitamin C depletion, comparing to those of wild type or vitamin C-supplemented Gulo(-/-) mice. To investigate the function of NKDC, CD49b+cells from splenocytes were isolated by MACS and then analyzed by flow cytometry. As a result, the number of NKDC population was completely reduced in vitamin C depleted Gulo(-/-) mice, which showed decrease of a secretion of IFN-gamma ƒ×. In addition, Fms-like tyrosine kinase 3 ligand (Flt3L), which stimulates the expansion and differentiation of IKDCs, was significantly reduced in vitamin C depleted Gulo(-/-) mice serum. Finally, the anti-tumor function of splenocytes from vitamin C depleted Gulo(-/-) mice was decreased, compared with those of wild type or vitamin C supplemented Gulo(-/-) mice. Taken together, it suggests that vitamin C shows their anti-tumor activity through the maintenance of Flt3L and the number of IKDC population.
Type of Medium:
Online Resource
ISSN:
0022-1767
,
1550-6606
DOI:
10.4049/jimmunol.182.Supp.40.20
Language:
English
Publisher:
The American Association of Immunologists
Publication Date:
2009
detail.hit.zdb_id:
1475085-5
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