In:
British Journal of Nutrition, Cambridge University Press (CUP), Vol. 127, No. 11 ( 2022-06-14), p. 1621-1630
Abstract:
Enterohepatic circulation of 12 α -hydroxylated (12 α OH) bile acid (BA) is enhanced depending on the energy intake in high-fat diet-fed rats. Such BA metabolism can be reproduced using a diet supplemented with cholic acid (CA), which also induces simple steatosis, without inflammation and fibrosis, accompanied by some other symptoms that are frequently observed in the condition of non-alcoholic fatty liver in rats. We investigated whether supplementation of the diet with raffinose (Raf) improves hepatic lipid accumulation induced by the CA-fed condition in rats. After acclimation to the AIN-93-based control diet, male Wistar rats were fed diets supplemented with a combination of Raf (30 g/kg diet) and/or CA (0·5 g/kg diet) for 4 weeks. Dietary Raf normalised hepatic TAG levels (two-way ANOVA P 〈 0·001 for CA, P = 0·02 for Raf and P = 0·004 for interaction) in the CA-supplemented diet-fed rats. Dietary Raf supplementation reduced hepatic 12 α OH BA concentration (two-way ANOVA P 〈 0·001 for CA, P = 0·003 for Raf and P = 0·03 for interaction). The concentration of 12 α OH BA was reduced in the aortic and portal plasma. Raf supplementation increased acetic acid concentration in the caecal contents (two-way ANOVA P = 0·001 as a main effect). Multiple regression analysis revealed that concentrations of aortic 12 α OH BA and caecal acetic acid could serve as predictors of hepatic TAG concentration ( R 2 = 0·55, P 〈 0·001). However, Raf did not decrease the secondary 12 α OH BA concentration in the caecal contents as well as the transaminase activity in the CA diet-fed rats. These results imply that dietary Raf normalises hepatic lipid accumulation via suppression of enterohepatic 12 α OH BA circulation.
Type of Medium:
Online Resource
ISSN:
0007-1145
,
1475-2662
DOI:
10.1017/S0007114521002610
Language:
English
Publisher:
Cambridge University Press (CUP)
Publication Date:
2022
detail.hit.zdb_id:
2016047-1
SSG:
12
SSG:
21
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