In:
PLOS Genetics, Public Library of Science (PLoS), Vol. 19, No. 2 ( 2023-2-10), p. e1010628-
Abstract:
Temperature greatly affects numerous biological processes in all organisms. How multicellular organisms respond to and are impacted by hypothermic stress remains elusive. Here, we found that cold-warm stimuli induced depletion of the RNA exosome complex in the nucleoli but enriched it in the nucleoplasm. To further understand the function and mechanism of cold-warm stimuli, we conducted forward genetic screening and identified ZTF-7, which is required for RNA exosome depletion from nucleoli upon transient cold-warm exposure in C . elegans . ZTF-7 is a putative ortholog of human ZNF277 that may contribute to language impairments. Immunoprecipitation followed by mass spectrometry (IP-MS) found that ZTF-7 interacted with RPS-2, which is a ribosomal protein of the small subunit and participates in pre-rRNA processing. A partial depletion of RPS-2 and other proteins of the small ribosomal subunit blocked the cold-warm stimuli-induced reduction of exosome subunits from the nucleoli. These results established a novel mechanism by which C . elegans responds to environmental cold-warm exposure.
Type of Medium:
Online Resource
ISSN:
1553-7404
DOI:
10.1371/journal.pgen.1010628
DOI:
10.1371/journal.pgen.1010628.g001
DOI:
10.1371/journal.pgen.1010628.g002
DOI:
10.1371/journal.pgen.1010628.g003
DOI:
10.1371/journal.pgen.1010628.g004
DOI:
10.1371/journal.pgen.1010628.g005
DOI:
10.1371/journal.pgen.1010628.g006
DOI:
10.1371/journal.pgen.1010628.g007
DOI:
10.1371/journal.pgen.1010628.s001
DOI:
10.1371/journal.pgen.1010628.s002
DOI:
10.1371/journal.pgen.1010628.s003
DOI:
10.1371/journal.pgen.1010628.s004
DOI:
10.1371/journal.pgen.1010628.r001
DOI:
10.1371/journal.pgen.1010628.r002
DOI:
10.1371/journal.pgen.1010628.r003
DOI:
10.1371/journal.pgen.1010628.r004
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2023
detail.hit.zdb_id:
2186725-2
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