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  • 1
    In: Arthritis & Rheumatism, Wiley, Vol. 65, No. 8 ( 2013-08), p. 2037-2047
    Abstract: Lysophosphatidic acid (LPA) is a bioactive lipid that binds to a group of cell surface G protein–coupled receptors (LPA receptors 1–6 [LPA 1–6 ]) and has been implicated as an important mediator of angiogenesis, inflammation, and cancer growth. This study was undertaken to analyze the effects of LPA 1 on the development of arthritis. Methods Expression of LPA receptors on synovial tissue was analyzed by immunohistochemistry and quantitative reverse transcription–polymerase chain reaction. The effects of abrogation of LPA 1 on collagen‐induced arthritis (CIA) were evaluated using LPA 1 ‐deficient mice or LPA 1 antagonist. Migrating fluorescence‐labeled CD11b+ splenocytes, which were transferred into the synovium of mice with CIA, were counted. CD4+ naive T cells were incubated under Th1‐, Th2‐, or Th17‐polarizing conditions, and T helper cell differentiation was assessed. Osteoclast formation from bone marrow cells was examined. Results LPA 1 was highly expressed in the synovium of patients with rheumatoid arthritis (RA) compared with that of patients with osteoarthritis. LPA 1 ‐deficient mice did not develop arthritis following immunization with type II collagen (CII). LPA 1 antagonist also ameliorated murine CIA. Abrogation of LPA 1 was associated with reductions in cell infiltration, bone destruction in the joints, and interleukin‐17 production from CII‐stimulated splenocytes. Infiltration of transferred CD11b+ macrophages from LPA 1 ‐deficient mice into the synovium was suppressed compared with infiltration of macrophages from wild‐type mice. LPA 1 antagonist inhibited the infiltration of macrophages from wild‐type mice. Differentiation into Th17, but not Th1 or Th2, and osteoclast formation were also suppressed under conditions of LPA 1 deficiency or LPA 1 inhibition in vitro. Conclusion Collectively, these results indicate that LPA/LPA 1 signaling contributes to the development of arthritis via cellular infiltration, Th17 differentiation, and osteoclastogenesis. Thus, LPA 1 may be a promising target molecule for RA therapy.
    Type of Medium: Online Resource
    ISSN: 0004-3591 , 1529-0131
    URL: Issue
    RVK:
    RVK:
    Language: English
    Publisher: Wiley
    Publication Date: 2013
    detail.hit.zdb_id: 2014367-9
    detail.hit.zdb_id: 2754614-7
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  • 2
    In: Journal of Oral Pathology & Medicine, Wiley, Vol. 51, No. 8 ( 2022-09), p. 710-720
    Abstract: Polymorphous adenocarcinoma is a common intraoral minor salivary gland carcinoma in Western countries but is extremely rare in Japan. The current study aimed to characterize the clinicopathological features and status of molecular alterations of polymorphous adenocarcinoma‐associated genes, such as PRKD1/2/3 , ARID1A , and DDX3X , in a large cohort of Japanese patients with polymorphous adenocarcinoma. Methods We examined the cases of 36 Japanese patients with salivary gland polymorphous adenocarcinoma and 26 cases involving histopathological mimics. To detect gene splits, fluorescence in situ hybridization was carried out for polymorphous adenocarcinoma‐associated genes. Additionally, we applied a SNaPshot multiplex assay to identify PRKD1 hotspot mutations. Results This study revealed the indolent clinical course of polymorphous adenocarcinoma with a high 10‐year overall survival rate (92.9%), accompanied by occasional local recurrences and cervical lymph node metastasis (23.3%). Twenty cases (55.6%) of polymorphous adenocarcinoma (but none of the mimics) exhibited alterations in at least one polymorphous adenocarcinoma‐associated gene. Rearrangement of polymorphous adenocarcinoma‐associated genes and PRKD1 E710D were identified in 17 (47.2%) and 4 (11.1%) cases, respectively; one case showed coexisting PRKD3 split and PRKD1 E710D. In the multivariate analysis, high clinical stage ( p  = 0.0005), the presence of prominent nucleoli ( p  = 0.0003), and ARID1A split positivity ( p  = 0.004) were independent risk factors for disease‐free survival. Conclusion Japanese patients with polymorphous adenocarcinoma showed clinicopathological features similar to those reported in Western countries. This study disclosed that polymorphous adenocarcinoma‐associated genetic alterations were common and specific findings in polymorphous adenocarcinomas. The diagnostic role and possible prognostic significance of polymorphous adenocarcinoma‐associated genetic alterations in polymorphous adenocarcinomas were suggested.
    Type of Medium: Online Resource
    ISSN: 0904-2512 , 1600-0714
    URL: Issue
    Language: English
    Publisher: Wiley
    Publication Date: 2022
    detail.hit.zdb_id: 2026385-5
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  • 3
    Online Resource
    Online Resource
    Scientific Research Publishing, Inc. ; 2018
    In:  International Journal of Medical Physics, Clinical Engineering and Radiation Oncology Vol. 07, No. 01 ( 2018), p. 115-130
    In: International Journal of Medical Physics, Clinical Engineering and Radiation Oncology, Scientific Research Publishing, Inc., Vol. 07, No. 01 ( 2018), p. 115-130
    Type of Medium: Online Resource
    ISSN: 2168-5436 , 2168-5444
    Language: Unknown
    Publisher: Scientific Research Publishing, Inc.
    Publication Date: 2018
    detail.hit.zdb_id: 2681387-7
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  • 4
    In: Medical Physics, Wiley, Vol. 41, No. 10 ( 2014-10)
    Abstract: The Vero4DRT has a maximum field size of 150.0 × 150.0 mm. The purpose of the present study was to develop expanded‐field irradiation techniques using the unique gimbaled x‐ray head of the Vero4DRT and to evaluate the dosimetric characteristics thereof. Methods: Two techniques were developed. One features gimbal swing irradiation and multiple static segments consisting of four separate fields exhibiting 2.39° gimbal rotation around two orthogonal axes. The central beam axis for each piecewise‐field is shifted 40 mm from the isocenters of the left–right (LR) and superior–inferior (SI) directions, and, thus, the irradiation field size is expanded to 230.8 × 230.8 mm. Adjacent regions were created at the isocenter (a center‐adjacent expandedfield) and 20 mm from the isocenter (an off‐adjacent expandedfield). The field gaps or overlaps of combined piecewise‐fields were established by adjustment of gimbal rotation and movement of the multileaf collimator (MLC). Another technique features dynamic segment irradiation in which the beam is delivered while rotating the gimbal. The dose profile is controlled by a combination of gimbal swing motion and opening and closing of the MLC. This enabled the authors to expand the irradiation field on the LR axis because the direction of MLC motion is parallel to that axis. A field 220.6 × 150.0 mm in dimensions was configured and examined. To evaluate the dosimetric characteristics of the expandedfields, films inserted into water‐equivalent phantoms at depths of 50, 100, and 150 mm were irradiated and field sizes, penumbrae, flatness, and symmetry analyzed. In addition, the expanded‐field irradiation techniques were applied to intensity‐modulated radiation therapy (IMRT). A head‐and‐neck IMRT field, created using a conventional Linac (the Varian Clinac iX), was reproduced employing an expanded‐field of the Vero4DRT. The simulated dose distribution for the expanded‐IMRT field was compared to the measured dose distribution. Results: The field sizes, penumbrae, flatness, and symmetry of the center‐ and off‐adjacent expanded‐fields were 230.2–232.1 mm, 6.8–10.7 mm, 2.3%–5.1%, and −0.5% to −0.4%, respectively, at a depth of 100 mm. Similarly, the field sizes, penumbrae, flatness, and symmetry of dynamic segment irradiation on the LR axis were 219.2 mm, 6.0–6.2 mm, 3.4%, and −0.1%, respectively, at a depth of 100 mm. In the area of expanded‐IMRT dose distribution, the passing rate of 5% dose difference was 85.8% between measurements and simulation, and the 3%/3 mm gamma passing rate was 96.4%. Conclusions: Expanded‐field irradiation techniques were developed using a gimbaled x‐ray head. The techniques effectively extend target areas, as required when whole‐breast irradiation or head‐and‐neck IMRT is contemplated.
    Type of Medium: Online Resource
    ISSN: 0094-2405 , 2473-4209
    Language: English
    Publisher: Wiley
    Publication Date: 2014
    detail.hit.zdb_id: 1466421-5
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  • 5
    In: Journal of Applied Clinical Medical Physics, Wiley, Vol. 16, No. 2 ( 2015-03), p. 73-86
    Abstract: A novel three‐dimensional unicursal irradiation technique “Dynamic WaveArc” (DWA), which employs simultaneous and continuous gantry and O‐ring rotation during dose delivery, has been implemented in Vero4DRT. The purposes of this study were to develop a commissioning and quality assurance procedure for DWA irradiation, and to assess the accuracy of the mechanical motion and dosimetric control of Vero4DRT. To determine the mechanical accuracy and the dose accuracy with DWA irradiation, 21 verification test patterns with various gantry and ring rotational directions and speeds were generated. These patterns were irradiated while recording the irradiation log data. The differences in gantry position, ring position, and accumulated MU ( , and , respectively) between the planned and actual values in the log at each time point were evaluated. Furthermore, the doses delivered were measured using an ionization chamber and spherical phantom. The constancy of radiation output during DWA irradiation was examined by comparison with static beam irradiation. The mean absolute error (MAE) of and were within 0.1° and the maximum error was within 0.2°. The MAE of was within 0.7 MU, and maximum error was 2.7 MU. Errors of accumulated MU were observed only around control points, changing gantry, and ring velocity. The gantry rotational range, in which was greater than or equal to 2.0 MU, was not greater than 3.2%. It was confirmed that the extent of the large differences in accumulated MU was negligibly small during the entire irradiation range. The variation of relative output value for DWA irradiation was within 0.2%, and this was equivalent to conventional arc irradiation with a rotating gantry. In conclusion, a verification procedure for DWA irradiation was designed and implemented. The results demonstrated that Vero4DRT has adequate mechanical accuracy and beam output constancy during gantry and ring rotation. PACS number: 87
    Type of Medium: Online Resource
    ISSN: 1526-9914 , 1526-9914
    Language: English
    Publisher: Wiley
    Publication Date: 2015
    detail.hit.zdb_id: 2010347-5
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  • 6
    In: Medical Physics, Wiley, Vol. 43, No. 12 ( 2016-12), p. 6364-6374
    Abstract: The purposes of this study were two‐fold: first, to develop a four‐axis moving phantom for patient‐specific quality assurance (QA) in surrogate signal‐based dynamic tumor‐tracking intensity‐modulated radiotherapy (DTT‐IMRT), and second, to evaluate the accuracy of the moving phantom and perform patient‐specific dosimetric QA of the surrogate signal‐based DTT‐IMRT. Methods: The four‐axis moving phantom comprised three orthogonal linear actuators for target motion and a fourth one for surrogate motion. The positional accuracy was verified using four laser displacement gauges under static conditions (±40 mm displacements along each axis) and moving conditions [eight regular sinusoidal and fourth‐power‐of‐sinusoidal patterns with peak‐to‐peak motion ranges ( H ) of 10–80 mm and a breathing period ( T ) of 4 s, and three irregular respiratory patterns with H of 1.4–2.5 mm in the left–right, 7.7–11.6 mm in the superior‐inferior, and 3.1–4.2 mm in the anterior–posterior directions for the target motion, and 4.8–14.5 mm in the anterior–posterior direction for the surrogate motion, and T of 3.9–4.9 s]. Furthermore, perpendicularity, defined as the vector angle between any two axes, was measured using an optical measurement system. The reproducibility of the uncertainties in DTT‐IMRT was then evaluated. Respiratory motions from 20 patients acquired in advance were reproduced and compared three‐dimensionally with the originals. Furthermore, patient‐specific dosimetric QAs of DTT‐IMRT were performed for ten pancreatic cancer patients. The doses delivered to Gafchromic films under tracking and moving conditions were compared with those delivered under static conditions without dose normalization. Results: Positional errors of the moving phantom under static and moving conditions were within 0.05 mm. The perpendicularity of the moving phantom was within 0.2° of 90°. The differences in prediction errors between the original and reproduced respiratory motions were −0.1 ± 0.1 mm for the lateral direction, −0.1 ± 0.2 mm for the superior‐inferior direction, and −0.1 ± 0.1 mm for the anterior–posterior direction. The dosimetric accuracy showed significant improvements, of 92.9% ± 4.0% with tracking versus 69.8% ± 7.4% without tracking, in the passing rates of γ with the criterion of 3%/1 mm ( p 〈 0.001). Although the dosimetric accuracy of IMRT without tracking showed a significant negative correlation with the 3D motion range of the target ( r = − 0.59, p 〈 0.05), there was no significant correlation for DTT‐IMRT ( r = 0.03, p = 0.464). Conclusions: The developed four‐axis moving phantom had sufficient accuracy to reproduce patient respiratory motions, allowing patient‐specific QA of the surrogate signal‐based DTT‐IMRT under realistic conditions. Although IMRT without tracking decreased the dosimetric accuracy as the target motion increased, the DTT‐IMRT achieved high dosimetric accuracy.
    Type of Medium: Online Resource
    ISSN: 0094-2405 , 2473-4209
    Language: English
    Publisher: Wiley
    Publication Date: 2016
    detail.hit.zdb_id: 1466421-5
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  • 7
    In: Radiotherapy and Oncology, Elsevier BV, Vol. 111, No. 2 ( 2014-05), p. 301-305
    Type of Medium: Online Resource
    ISSN: 0167-8140
    Language: English
    Publisher: Elsevier BV
    Publication Date: 2014
    detail.hit.zdb_id: 1500707-8
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  • 8
    In: Medical Physics, Wiley, Vol. 41, No. 3 ( 2014-03)
    Abstract: The Vero4DRT system has the capability for dynamic tumor‐tracking (DTT) stereotactic irradiation using a unique gimbaled x‐ray head. The purposes of this study were to develop DTT conformal arc irradiation and to estimate its geometric and dosimetric accuracy. Methods: The gimbaled x‐ray head, supported on an O‐ring gantry, was moved in the pan and tilt directions during O‐ring gantry rotation. To evaluate the mechanical accuracy, the gimbaled x‐ray head was moved during the gantry rotating according to input command signals without a target tracking, and a machine log analysis was performed. The difference between a command and a measured position was calculated as mechanical error. To evaluate beam‐positioning accuracy, a moving phantom, which had a steel ball fixed at the center, was driven based on a sinusoidal wave (amplitude [A]: 20 mm, time period [T] : 4 s), a patient breathing motion with a regular pattern (A: 16 mm, average T: 4.5 s), and an irregular pattern (A: 7.2–23.0 mm, T: 2.3–10.0 s), and irradiated with DTT during gantry rotation. The beam‐positioning error was evaluated as the difference between the centroid position of the irradiated field and the steel ball on images from an electronic portal imaging device. For dosimetric accuracy, dose distributions in static and moving targets were evaluated with DTT conformal arc irradiation. Results: The root mean squares (RMSs) of the mechanical error were up to 0.11 mm for pan motion and up to 0.14 mm for tilt motion. The RMSs of the beam‐positioning error were within 0.23 mm for each pattern. The dose distribution in a moving phantom with tracking arc irradiation was in good agreement with that in static conditions. Conclusions: The gimbal positional accuracy was not degraded by gantry motion. As in the case of a fixed port, the Vero4DRT system showed adequate accuracy of DTT conformal arc irradiation.
    Type of Medium: Online Resource
    ISSN: 0094-2405 , 2473-4209
    Language: English
    Publisher: Wiley
    Publication Date: 2014
    detail.hit.zdb_id: 1466421-5
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  • 9
    Online Resource
    Online Resource
    Japan Society of Mechanical Engineers ; 2021
    In:  The Proceedings of the Fluids engineering conference Vol. 2021, No. 0 ( 2021), p. OS07-19-
    In: The Proceedings of the Fluids engineering conference, Japan Society of Mechanical Engineers, Vol. 2021, No. 0 ( 2021), p. OS07-19-
    Type of Medium: Online Resource
    ISSN: 2424-2896
    Language: English
    Publisher: Japan Society of Mechanical Engineers
    Publication Date: 2021
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  • 10
    Online Resource
    Online Resource
    Japan Society of Mechanical Engineers ; 2002
    In:  The Proceedings of Conference of Kansai Branch Vol. 2002.77, No. 0 ( 2002), p. _6-45_-_6-46_
    In: The Proceedings of Conference of Kansai Branch, Japan Society of Mechanical Engineers, Vol. 2002.77, No. 0 ( 2002), p. _6-45_-_6-46_
    Type of Medium: Online Resource
    ISSN: 2424-2756
    Uniform Title: 620 斜流ポンプの内部流れ
    Language: English , Japanese
    Publisher: Japan Society of Mechanical Engineers
    Publication Date: 2002
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