In:
Development, Growth & Differentiation, Wiley, Vol. 45, No. 5-6 ( 2003-10), p. 507-514
Abstract:
Five putative Ca 2+ ‐binding proteins, CBP5, 6, 7, 8 and 9, all having EF‐hand motifs, were found by searching the Dictyostelium cDNA database ( http://www.csm.biol.tsukuba.ac.jp/cDNAproject.html ). 45 Ca 2+ ‐overlay experiments revealed that four of these (excluding CBP9) are real Ca 2+ ‐binding proteins. Northern blot analysis revealed that the genes encoding CBP5, 6, 7 and 8 are all developmentally regulated. In situ hybridization analyses revealed that spatial expression of these genes was regulated in several different ways. CBP1, 2, 3, 5, 6 and 7 are expressed in prespore cells in the slug stage. Transcripts of the genes for CBP1 and 5 are enriched in prestalk subtype PstO cells. In contrast, CBP4 is expressed predominantly in PstO cells. CBP8 is evenly expressed at a very low level throughout the whole slug. Such distinct spatial expression patterns suggest that the CBP might be involved in morphogenesis and might have their own roles either in prespore or in prestalk cell differentiation of Dictyostelium .
Type of Medium:
Online Resource
ISSN:
0012-1592
,
1440-169X
DOI:
10.1111/dgd.2003.45.issue-5-6
DOI:
10.1111/j.1440-169X.2003.00718.x
Language:
English
Publisher:
Wiley
Publication Date:
2003
detail.hit.zdb_id:
2020067-5
SSG:
12
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