In:
International Journal of Systematic and Evolutionary Microbiology, Microbiology Society, Vol. 70, No. 4 ( 2020-04-01), p. 2648-2656
Abstract:
From a phlegmon in a dog an aerobic and facultatively anaerobic, indole-, oxidase- and catalase-negative, non-motile bacterium was isolated in 2019 in Germany that stained Gram-negative and showed a pleomorphic, rod-shaped, non-spore-forming appearance. Based on the results of 16S rRNA gene sequence analyses, strain IHIT1603-19 T was assigned to the genus Streptobacillus with sequence similarities of 98.6, 98.0, 97.9, 97.1 and 94.4 % to the type strains of Streptobacillus felis , Streptobacillus notomytis , Streptobacillus ratti , Streptobacillus moniliformis and Streptobacillus hongkongensis , respectively. Strain IHIT1603-19 T could also clearly be differentiated from other Streptobacillus species by rpoB , groEL and recA gene, nucleotide and amino acid sequence analyses as well as by core genome phylogeny. Regarding DNA–DNA relatedness, strain IHIT1603-19 T demonstrated an average nucleotide identity of 83.00 and 82.28 % compared to S. felis 131000547 T and S. moniliformis DSM 12112 T , respectively. Chemotaxonomic and physiological data of strain IHIT1603-19 T were in congruence with other closely related members of the family Leptotrichiaceae , represented by highly similar enzyme profiles and fatty acid patterns. MALDI-TOF MS analysis also proved suitable in unequivocally discriminating strain IHIT1603-19 T from all currently described taxa of the genus Streptobacillus . On the basis of these data, we propose the novel species Streptobacillus canis sp. nov. with the type strain IHIT1603-19 T (=DSM 110501 T =CCUG 74118 T =CIP 111795 T ). The G+C content of the DNA of the type strain is 26.6 mol%, genome size is 1.60 Mbp.
Type of Medium:
Online Resource
ISSN:
1466-5026
,
1466-5034
DOI:
10.1099/ijsem.0.004086
Language:
English
Publisher:
Microbiology Society
Publication Date:
2020
detail.hit.zdb_id:
215062-1
detail.hit.zdb_id:
2056611-6
SSG:
12
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