In:
PLOS Pathogens, Public Library of Science (PLoS), Vol. 19, No. 4 ( 2023-4-26), p. e1011320-
Abstract:
Viral seasonality in the aquaculture industry is an important scientific issue for decades. While the molecular mechanisms underpinning the temperature-dependent pathogenesis of aquatic viral diseases remain largely unknown. Here we report that temperature-dependent activation of IL6-STAT3 signaling was exploited by grass carp reovirus (GCRV) to promote viral entry via increasing the expression of heat shock protein 90 (HSP90). Deploying GCRV infection as a model system, we discovered that GCRV induces the IL6-STAT3-HSP90 signaling activation to achieve temperature-dependent viral entry. Further biochemical and microscopic analyses revealed that the major capsid protein VP7 of GCRV interacted with HSP90 and relevant membrane-associated proteins to boost viral entry. Accordingly, exogenous expression of either IL6, HSP90, or VP7 in cells increased GCRV entry in a dose-dependent manner. Interestingly, other viruses (e.g., koi herpesvirus, Rhabdovirus carpio, Chinese giant salamander iridovirus) infecting ectothermic vertebrates have evolved a similar mechanism to promote their infection. This work delineates a molecular mechanism by which an aquatic viral pathogen exploits the host temperature-related immune response to promote its entry and replication, instructing us on new ways to develop targeted preventives and therapeutics for aquaculture viral diseases.
Type of Medium:
Online Resource
ISSN:
1553-7374
DOI:
10.1371/journal.ppat.1011320
DOI:
10.1371/journal.ppat.1011320.g001
DOI:
10.1371/journal.ppat.1011320.g002
DOI:
10.1371/journal.ppat.1011320.g003
DOI:
10.1371/journal.ppat.1011320.g004
DOI:
10.1371/journal.ppat.1011320.g005
DOI:
10.1371/journal.ppat.1011320.g006
DOI:
10.1371/journal.ppat.1011320.g007
DOI:
10.1371/journal.ppat.1011320.s001
DOI:
10.1371/journal.ppat.1011320.s002
DOI:
10.1371/journal.ppat.1011320.s003
DOI:
10.1371/journal.ppat.1011320.s004
DOI:
10.1371/journal.ppat.1011320.s005
DOI:
10.1371/journal.ppat.1011320.s006
DOI:
10.1371/journal.ppat.1011320.s007
DOI:
10.1371/journal.ppat.1011320.s008
DOI:
10.1371/journal.ppat.1011320.s009
DOI:
10.1371/journal.ppat.1011320.s010
DOI:
10.1371/journal.ppat.1011320.s011
DOI:
10.1371/journal.ppat.1011320.s012
DOI:
10.1371/journal.ppat.1011320.s013
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2023
detail.hit.zdb_id:
2205412-1
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