In:
Vietnam Journal of Biotechnology, Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications), Vol. 19, No. 4 ( 2022-05-03), p. 659-666
Abstract:
Pelodiscus sinensis is a widespread species of soft-shelled turtle that is commercially cultured in freshwater and prominently exploited as speciality food in Vietnam. During the cultivation, there are distinctly recurrent epidemic diseases causing economically vast damage for farmers. In this research, we carried out the isolation of microorganisms from typical ulcers “ba dau” presenting on soft-shelled turtle taken in Nam Dinh farm. The samples were inoculated on common media used for bacteria, actinomyces, fungus and yeast. Only bacteria grew on typical culture medium. Fifteen bacterial strains were isolated and identified by 16S rRNA sequence analysis. They were belonged to genera such as Acidovorax (n=1), Acinetobacter (n=1), Aeromonas (n=8), Citrobacter (n=1), Chryseobacterium (n=1), Moraxella (n=1), Paludibacterium (n=1) and Parabacteroides (n=1). In which, eight strains Aeromonas spp. were selected for further research on virulence characterization. PCR results showed that these strains possessed at least 4/7 virulence genes including aer (75%), alt (37.5%), ast (50%), ela (87.5%), exu ( 100%), hlyA (37.5%) and ser (100%). With additional tests, we confirmed the virulent expression by haemolysin (100%), protease (75%), DNAase (75%), elastase (25%) and cytotoxicity (62.5%). These results are scientific basis to establish and develop practical methods for controlling and preventing "ba dau" disease in the soft-shelled turtle in the future.
Type of Medium:
Online Resource
ISSN:
1811-4989
,
1811-4989
DOI:
10.15625/1811-4989/15681
Language:
Unknown
Publisher:
Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications)
Publication Date:
2022
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