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  • 1
    Online Resource
    Online Resource
    Shear Wave Elastography-Assisted Ultrasound Breast Image Analysis and Identification of Abnormal Data
    Wiley ; 2022
    In:  Journal of Healthcare Engineering Vol. 2022 ( 2022-1-7), p. 1-10
    Details
    In: Journal of Healthcare Engineering, Wiley, Vol. 2022 ( 2022-1-7), p. 1-10
    Abstract: In this paper, shear wave elastography was used to study and analyze the images of the breast in-depth and identify the abnormal image data. Sixty breast lesions were evaluated, and quantitative metrics were reproducible in the static and dynamic modes of shear wave elastography with a higher interobserver agreement in dynamic qualitative metrics than in the static mode. There were no statistically significant differences between the two modes of imaging in quantitative metrics, and quantitative metrics were more effective than qualitative metrics. Postoperative immunohistochemical expression of ER, PR, HER-2, Ki-67, molecular typing, pathological type, histological grading, and axillary lymph node status of breast cancer was obtained based on pathological results. The correlation between mass size, patient age, and WiMAX values of breast cancer masses was analyzed using Pearson correlation, and the differences in SWVmax values of breast cancer masses between different expressions of immunohistochemical parameters ER, PR, HER-2, Ki-67, and axillary lymph node status were compared using tests. The variables with correlations and differences were included in the multiple linear regression analysis to assess the factors influencing the SWVmax values. The performance of TDPM, SPM, and TSPM was compared using PVA body models with different freeze-thaw cycles. The results showed that TSPM performed better than SPM in general, and TDPM showed excellent performance because of high temporal resolution and low random error, especially when the number of freeze-thaw cycles increased and the hardness of the PVA body mold increased. Measurements at different depths of inhomogeneous body models also showed that the TDPM method was less affected by depth, and the results were more stable. Finally, the reliability of the shear wave velocity (SWS) measured by the TDPM and SPM methods was investigated using porcine ligament tissue, and the results showed that the mean values of SWS goodness of fit for TDPM and SPM were 0.94 and 0.87, respectively, and the estimated elastic modulus of TDPM was very close to the mechanical test results.
    Type of Medium: Online Resource
    ISSN: 2040-2309 , 2040-2295
    URL: Article
    DOI: 10.1155/2022/5499354
    Language: English
    Publisher: Wiley
    Publication Date: 2022
    detail.hit.zdb_id: 2545054-2
    detail.hit.zdb_id: 2571779-0
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  • 2
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    Plasma metabolic signatures for intracranial aneurysm and its rupture identified by pseudotargeted metabolomics
    Elsevier BV ; 2023
    In:  Clinica Chimica Acta Vol. 538 ( 2023-01), p. 36-45
    Details
    In: Clinica Chimica Acta, Elsevier BV, Vol. 538 ( 2023-01), p. 36-45
    Type of Medium: Online Resource
    ISSN: 0009-8981
    URL: Article
    DOI: 10.1016/j.cca.2022.11.002
    Language: English
    Publisher: Elsevier BV
    Publication Date: 2023
    detail.hit.zdb_id: 80228-1
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  • 3
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    Clinical Characteristics and Early Interventional Responses in Patients with Severe COVID-19 Pneumonia
    Wiley ; 2021
    In:  Computational and Mathematical Methods in Medicine Vol. 2021 ( 2021-5-31), p. 1-6
    Details
    In: Computational and Mathematical Methods in Medicine, Wiley, Vol. 2021 ( 2021-5-31), p. 1-6
    Abstract: Progressive acute respiratory distress syndrome (ARDS) is the most lethal cause in patients with severe COVID-19 pneumonia due to uncontrolled inflammatory reaction, for which we found that early intervention of combined treatment with methylprednisolone and human immunoglobulin is a highly effective therapy to improve the prognosis of COVID-19-induced pneumonia patients. Objective. Herein, we have demonstrated the clinical manifestations, laboratory, and radiological characteristics of patients with severe Coronavirus Disease-2019 (COVID-19) pneumonia, as well as measures to ensure early diagnosis and intervention for improving clinical outcomes of COVID-19 patients. Summary Background Data. The COVID-19 is a new infection caused by a severe acute respiratory syndrome- (SARS-) like coronavirus that emerged in China in December 2019 and has claimed millions of lives. Methods. We included 37 severe COVID-19 pneumonia patients who were hospitalized at Taizhou Public Health Medical Center in Zhejiang province from January 17, 2020, to February 18, 2020. Demographic, clinical, and laboratory features; imaging characteristics; treatment history; and clinical outcomes of all patients were collected from electronic medical records. Results. The patients’ mean age was 54 years (interquartile range, 43−64), with a slightly higher male preponderance (57%). The most common clinical features of COVID-19 pneumonia were fever (29 (78%)), dry cough (28 (76%)), dyspnea (9 (24%)), and fatigue (9 (24%)). Serum interleukin (IL)-6 and IL-10 were elevated in 35 (95%) and 19 (51%) patients, respectively. Chest computerized tomography scan revealed bilateral pneumonia in 35 (95%) patients. Early intervention with a combination of methylprednisolone and human immunoglobulin was highly effective in improving the prognosis of these patients. Conclusions. Progressive acute respiratory distress syndrome is the most common cause of death in patients with severe COVID-19 pneumonia owing to an uncontrolled inflammatory response. Early intervention with methylprednisolone and human immunoglobulin was highly effective in improving their prognosis.
    Type of Medium: Online Resource
    ISSN: 1748-6718 , 1748-670X
    URL: Article
    DOI: 10.1155/2021/6676987
    Language: English
    Publisher: Wiley
    Publication Date: 2021
    detail.hit.zdb_id: 2256917-0
    detail.hit.zdb_id: 2252430-7
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  • 4
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    Bioadsorption of Terbium(III) by Spores of Bacillus subtilis
    MDPI AG ; 2022
    In:  Minerals Vol. 12, No. 7 ( 2022-07-08), p. 866-
    Details
    In: Minerals, MDPI AG, Vol. 12, No. 7 ( 2022-07-08), p. 866-
    Abstract: Wastewater containing low concentrations of rare earth ions not only constitutes a waste of rare earth resources but also threatens the surrounding environment. It is therefore necessary to develop environmentally friendly methods of recovering rare earth ions. The spores produced by Bacillus are resistant to extreme environments and are effective in the bioadsorption of rare earth ions, but their adsorption behaviors and mechanisms are not well understood. In this study, the cells and spores of Bacillus subtilis PS533 and PS4150 were used as biosorbents, and their adsorption of terbium ions was compared under different conditions. The adsorption characteristics of the spores were investigated, as were the possible mechanisms of interaction between the spores and rare earth ions. The results showed that the PS4150 spores had the best adsorption effect on Tb(III), with the removal percentage reaching 95.2%. Based on a computational simulation, SEM observation, XRD, XPS, and FTIR analyses, it was suggested that the adsorption of Tb(III) by the spores conforms to the pseudo−second−order kinetics and the Langmuir adsorption isotherm model. This indicates that the adsorption process mainly consists of chemical adsorption, and that groups such as amino, hydroxyl, methyl, and phosphate, which are found on the surface of the spores, are involved in the bioadsorption process. All of these findings suggest that Bacillus subtilis spores can be used as a potential biosorbent for the recovery of rare earth ions from wastewater.
    Type of Medium: Online Resource
    ISSN: 2075-163X
    URL: Article
    DOI: 10.3390/min12070866
    Language: English
    Publisher: MDPI AG
    Publication Date: 2022
    detail.hit.zdb_id: 2655947-X
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  • 5
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    Online Resource
    Genetic, Molecular, and Pathogenic Characterization of the H9N2 Avian Influenza Viruses Currently Circulating in South China
    MDPI AG ; 2019
    In:  Viruses Vol. 11, No. 11 ( 2019-11-08), p. 1040-
    Details
    In: Viruses, MDPI AG, Vol. 11, No. 11 ( 2019-11-08), p. 1040-
    Abstract: The prevalence and variation of the H9N2 avian influenza virus (AIV) pose a threat to public health. A total of eight viruses isolated from farmed poultry in South China during 2017–2018 were selected as representative strains for further systematic study. Phylogenetic analyses indicated that these prevalent viruses belong to the Y280-like lineage and that the internal genes are highly similar to those of recently circulating human H7N9 viruses. The receptor-binding assay showed that most of the H9N2 isolates preferentially bound to the human-like receptor, increasing the risk of them crossing the species barrier and causing human infection. Our in vitro, multi-step growth curve results indicate these viruses can effectively replicate in mammalian cells. Infection in mice showed that three viruses effectively replicated in the lung of mice. Infection in swine revealed that the viruses readily replicated in the upper respiratory tract of pig and effectively induced viral shedding. Our findings suggested that the H9N2 AIVs circulating in poultry recently acquired an enhanced ability to transmit from avian to mammalians, including humans. Based on our findings, we propose that it is essential to strengthen the efforts to surveil and test the pathogenicity of H9N2 AIVs.
    Type of Medium: Online Resource
    ISSN: 1999-4915
    URL: Article
    DOI: 10.3390/v11111040
    Language: English
    Publisher: MDPI AG
    Publication Date: 2019
    detail.hit.zdb_id: 2516098-9
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  • 6
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    Identification of Three Campylobacter Lysins and Enhancement of Their Anti-Escherichia coli Efficacy Using Colicin-Based Translocation and Receptor-Binding Domain Fusion
    American Society for Microbiology ; 2023
    In:  Microbiology Spectrum Vol. 11, No. 2 ( 2023-04-13)
    Details
    In: Microbiology Spectrum, American Society for Microbiology, Vol. 11, No. 2 ( 2023-04-13)
    Abstract: The emergence of multidrug-resistant Escherichia coli , which poses a major threat to public health, has motivated the development of numerous alternative antimicrobials. Lysins are bacteriophage- and bacterium-derived peptidoglycan hydrolases that represent a new antibiotic treatment targeting bacterial cell walls. However, the bactericidal effect of native lysins on Gram-negative bacteria is restricted by the presence of an outer membrane. Here, we first evaluated the antibacterial activity of three Campylobacter -derived lysins (Clysins) against E. coli . To improve their transmembrane ability and antibacterial activities, six engineered Clysins were constructed by fusing with the translocation and receptor-binding (TRB) domains from two types of colicins (colicin A [TRBA] and colicin K [TRBK] ), and their biological activities were determined. Notably, engineered lysin TRBK-Cly02 exhibited the highest bactericidal activity against the E. coli BL21 strain, with a reduction of 6.22 ± 0.34 log units of cells at a concentration of 60.1 μg/mL, and formed an observable inhibition zone even at a dose of 6.01 μg. Moreover, TRBK-Cly02 killed E. coli dose dependently and exhibited the strongest bactericidal activity at pH 6. It also exhibited potential bioactivity against multidrug-resistant E. coli clinical isolates. In summary, this study identified three lysins from Campylobacter strains against E. coli , and the enhancement of their antibacterial activities by TRB domains fusion may allow them to be developed as potential alternatives to antibiotics. IMPORTANCE Three lysins from Campylobacter , namely, Clysins, were investigated, and their antibacterial activities against E. coli were determined for the first time. To overcome the restriction of the outer membrane of Gram-negative bacteria, we combined the TRB domains of colicins with these Clysins. Moreover, we discovered that the Clysins fused with TRB domains from colicin K (TRBK) killed E. coli more effectively, and this provides a new foundation for the development of novel bioengineered lysins by employing TRBK constructs that target outer membrane receptor/transport systems. One of the designed lysins, TRBK-Cly02, exhibited potent bactericidal efficacy against E. coli strains and may be used for control of multidrug-resistant clinical isolates. The results suggest that TRBK-Cly02 can be considered a potential antibacterial agent against pathogenic E. coli .
    Type of Medium: Online Resource
    ISSN: 2165-0497
    URL: Article
    DOI: 10.1128/spectrum.04515-22
    Language: English
    Publisher: American Society for Microbiology
    Publication Date: 2023
    detail.hit.zdb_id: 2807133-5
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  • 7
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    Preparation of Co‐based N‐doped Meso‐microporous Carbon for Hydrogenation of Nitroarenes
    Wiley ; 2022
    In:  ChemistrySelect Vol. 7, No. 42 ( 2022-11-11)
    Details
    In: ChemistrySelect, Wiley, Vol. 7, No. 42 ( 2022-11-11)
    Abstract: The development of high‐efficiency and low‐cost heterogeneous catalysts with superior selectivity for the reduction of nitro compounds is desirable. Herein, we prepared Co‐based nitrogen‐doped meso‐microporous carbon (Co/N−C) derived from polyvinylpyrrolidone (PVP)‐coated zeolitic imidazolate framework (ZIF)‐67 as a heterogeneous catalyst for the synthesis of aromatic amines via in‐situ restricted pyrolysis. The structure, morphology, and catalytic activity of Co/N−C were investigated, and the results indicated that Co/N−C‐800 has the combined advantage of the excellent catalytic activity of cobalt nanoparticles and the high mass transfer rate of the N‐doped meso‐microporous carbon framework, achieving superior catalytic ability for the selective hydrogenation of 20 representative nitroaromatic compounds. Co/N−C‐800 was recyclable by simple separation due to the strong magnetism of the Co nanoparticles and exhibited excellent reusability, with 100 % yield of aniline even after six cycles. The catalytic reduction may be attributed to the dissociation of hydrazine to H + /H − via the action of Co/N−C.
    Type of Medium: Online Resource
    ISSN: 2365-6549 , 2365-6549
    URL: Issue
    URL: Article
    DOI: 10.1002/slct.v7.42
    DOI: 10.1002/slct.202202288
    Language: English
    Publisher: Wiley
    Publication Date: 2022
    detail.hit.zdb_id: 2844262-3
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  • 8
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    Online Resource
    Development of a Loop-Mediated Isothermal Amplification Assay for Rapid Detection of Subgroup J Avian Leukosis Virus
    American Society for Microbiology ; 2010
    In:  Journal of Clinical Microbiology Vol. 48, No. 6 ( 2010-06), p. 2116-2121
    Details
    In: Journal of Clinical Microbiology, American Society for Microbiology, Vol. 48, No. 6 ( 2010-06), p. 2116-2121
    Abstract: Infection of breeder flocks in China with subgroup J avian leukosis virus (ALV-J) has increased recently. In this study, we have developed a loop-mediated isothermal amplification (LAMP) assay for rapid detection of ALV-J from culture isolates and clinical samples. The ALV-J-specific LAMP assay efficiently amplified the target gene within 45 min at 63°C using only a simple laboratory water bath. To determine the specificity of the LAMP assay, various subgroup ALVs and other related viruses were detected. A ladder pattern on gel electrophoresis was observed for ALV-J isolates but not for other viruses. To evaluate the sensitivities of the LAMP assay and conventional PCR, the NX0101 isolate plasmid DNA was amplified by them. The detection limit of the LAMP assay was 5 target gene copies/reaction, which was up to 20 times higher than that of conventional PCR. To evaluate the application of the LAMP assay for detection of ALV-J in clinical samples, 49 samples suspected of ALV infection from breeder flocks were tested by the LAMP assay and PCR. Moreover, virus isolation from these samples was also performed using cell culture. The positive-sample ratios were 21/49 (43%) by conventional PCR, 26/49 (53%) by the LAMP assay, and 19/46 (41%) by virus isolation. Additionally, a positive LAMP reaction can be visually ascertained by the observation of turbidity or a color change after addition of SYBR green I dye. Consequently, the LAMP assay is a simple, rapid, and sensitive diagnostic method and can potentially be developed for rapid detection of ALV-J infection in the field.
    Type of Medium: Online Resource
    ISSN: 0095-1137 , 1098-660X
    URL: Article
    DOI: 10.1128/JCM.02530-09
    RVK:
    XA 10000
    Language: English
    Publisher: American Society for Microbiology
    Publication Date: 2010
    detail.hit.zdb_id: 1498353-9
    detail.hit.zdb_id: 390499-4
    SSG: 12
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  • 9
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    Complete Genome Sequence of an H5N2 Avian Influenza Virus Isolated from a Parrot in Southern China
    American Society for Microbiology ; 2012
    In:  Journal of Virology Vol. 86, No. 16 ( 2012-08-15), p. 8890-8891
    Details
    In: Journal of Virology, American Society for Microbiology, Vol. 86, No. 16 ( 2012-08-15), p. 8890-8891
    Abstract: We report the complete genome sequence of an H5N2 avian influenza virus (AIV) that was first isolated from a parrot in Guangdong in southern China in 2004. Genomic sequence and phylogenetic analyses showed that it was highly homologous with the North American H5N2 viruses and all eight genes of this virus belonged to the North American gene lineage. These data will help in the investigation of the epidemiology and host range of AIVs in southern China.
    Type of Medium: Online Resource
    ISSN: 0022-538X , 1098-5514
    URL: Article
    DOI: 10.1128/JVI.01189-12
    Language: English
    Publisher: American Society for Microbiology
    Publication Date: 2012
    detail.hit.zdb_id: 1495529-5
    detail.hit.zdb_id: 80174-4
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  • 10
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    Prevalence and genomic-based antimicrobial resistance analysis of Avibacterium paragallinarum isolates in Guangdong Province, China
    Elsevier BV ; 2024
    In:  Poultry Science Vol. 103, No. 6 ( 2024-06), p. 103751-
    Details
    In: Poultry Science, Elsevier BV, Vol. 103, No. 6 ( 2024-06), p. 103751-
    Type of Medium: Online Resource
    ISSN: 0032-5791
    URL: Article
    DOI: 10.1016/j.psj.2024.103751
    Language: English
    Publisher: Elsevier BV
    Publication Date: 2024
    detail.hit.zdb_id: 242586-5
    SSG: 22
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