In:
Journal of Clinical Periodontology, Wiley, Vol. 40, No. 10 ( 2013-10), p. 933-941
Abstract:
To determine the levels of LL ‐37 in and its susceptibility to degradation by components of gingival crevicular fluid ( GCF ) in periodontal health and disease. Materials and Methods Levels of LL ‐37 in GCF from periodontitis patients and periodontally healthy subjects were determined by ELISA . In addition, degradation of synthetic/exogenous LL ‐37 by components of GCF in the presence and absence of inhibitors was determined by matrix‐assisted laser desorption/ionization time of flight mass spectrometry. Results The concentration of native LL ‐37 in GCF from Porphyromonas gingivalis positive (Pg+) and P. gingivalis negative (Pg‐) sites in periodontitis patients was significantly higher than in GCF from healthy subjects. When synthetic LL ‐37 was added to healthy GCF , the peptide was not degraded. Conversely, GCF from Pg+ sites rapidly degraded synthetic LL ‐37 which was prevented in the presence of Arg− and Lys− gingipain inhibitors. Synthetic LL ‐37 was degraded more slowly by GCF from Pg− sites. Conclusions LL ‐37 is detectable in GCF in periodontal health and disease. The rapid degradation of synthetic LL ‐37 in periodontitis GCF , particularly in Pg+ sites, limits its role as a potential therapeutic in the gingival crevice. These results highlight the need to design stable peptide mimetics of LL ‐37 as future therapeutics in periodontitis.
Type of Medium:
Online Resource
ISSN:
0303-6979
,
1600-051X
DOI:
10.1111/jcpe.2013.40.issue-10
Language:
English
Publisher:
Wiley
Publication Date:
2013
detail.hit.zdb_id:
2026349-1
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