In:
American Journal of Reproductive Immunology, Wiley, Vol. 73, No. 5 ( 2015-05), p. 417-427
Abstract:
Thrombophilia is associated with pregnancy complications. Treatment with low molecular weight heparin ( LMWH ) improves pregnancy outcome, but the underlying mechanisms are not clear. Methods of study We analyzed T reg frequency in blood from thrombophilic pregnancies treated with LMWH ( n = 32) or untreated ( n = 33) and from healthy pregnancies ( n = 39) at all trimesters. Additionally, we treated pregnant wild‐type, heterozygous and homozygous factor‐ V ‐ L eiden ( FVL ) mice with LMWH or PBS and determined T reg frequency, pro‐/anti‐inflammatory cytokine levels and C aspase‐3‐activity in placenta and decidua. Results Treg frequencies were increased in second and third trimester in LMWH ‐treated thrombophilic pregnancies compared to controls. Treg levels were comparable to those of normal pregnancies. Homozygous FVL mice had decreased decidual Tregs compared to wild‐type mice. LMWH treatment normalized T regs and was associated with increased decidual IL ‐10 m RNA . LMWH diminished C aspase‐3‐activity in mice of all genotypes. Conclusion We demonstrated anti‐apoptotic and anti‐inflammatory effects of LMWH in pregnant FVL mice. LMWH increased T reg levels in mice and humans, which suggests benefits of LMWH treatment for thrombophilic women during pregnancy.
Type of Medium:
Online Resource
ISSN:
1046-7408
,
1600-0897
DOI:
10.1111/aji.2015.73.issue-5
Language:
English
Publisher:
Wiley
Publication Date:
2015
detail.hit.zdb_id:
2024667-5
Permalink