In:
Plant Biotechnology Journal, Wiley, Vol. 7, No. 8 ( 2009-10), p. 791-806
Abstract:
Generating a new variety of plant with erect‐leaf is a critical strategy to improve rice grain yield, as plants with this trait can be dense‐planted. The erect‐leaf is a significant morphological trait partially regulated by Brassinosteroids (BRs) in rice plants. So far, only a few genes can be used for molecular breeding in rice. Here, we identified OsBAK1 as a potential gene to alter rice architecture. Based on rice genome sequences, four closely related homologs of Arabidopsis BAK1 ( AtBAK1 ) gene were amplified. Phylogenetic analysis and suppression of a weak Arabidopsis mutant bri1‐5 indicated that OsBAK1 (Os08g0174700) is the closest relative of AtBAK1. Genetic, physiological, and biochemical analyses all suggest that the function of OsBAK1 is conserved with AtBAK1 . Overexpression of a truncated intracellular domain of OsBAK1 , but not the extracellular domain of OsBAK1 , resulted in a dwarfed phenotype, similar to the rice BR‐insensitive mutant plants. The expression of OsBAK1 changed important agricultural traits of rice such as plant height, leaf erectness, grain morphologic features, and disease resistance responses. Our results suggested that a new rice variety with erect‐leaf and normal reproduction can be generated simply by suppressing the expression level of OsBAK1 . Therefore, OsBAK1 is a potential molecular breeding tool for improving rice grain yield by modifying rice architecture.
Type of Medium:
Online Resource
ISSN:
1467-7644
,
1467-7652
DOI:
10.1111/pbi.2009.7.issue-8
DOI:
10.1111/j.1467-7652.2009.00444.x
Language:
English
Publisher:
Wiley
Publication Date:
2009
detail.hit.zdb_id:
2136367-5
SSG:
12
Permalink