In:
Frontiers in Molecular Biosciences, Frontiers Media SA, Vol. 8 ( 2021-3-11)
Abstract:
Hydrogen sulfide (H 2 S) is a gasotransmitter along with nitric oxide and carbon oxide, which is involved in plant growth and development as well as biotic and abiotic stress resistance. In a previous study, we reported that mitogen-activated protein kinases, especially MPK4, are important downstream components of H 2 S involved in alleviating cold stress; however the underlying mechanism is unclear. In this study, we determined that the ability of H 2 S to alleviate cold stress is impaired in mpk4 mutants, but not in the upstream mek2 and crlk1 mutants. MPK4 was basically persulfidated, and NaHS (H 2 S donor) further increased the persulfidation level of MPK4. MEK2 was not persulfidated by H 2 S. NaHS treatments increased the MPK4 activity level nearly tenfold. The persulfidation signal of MPK4 did not disappear after eight cystein residues in MPK4 were site-mutated, respectively. Above all, our results suggested that H 2 S alleviates cold stress directly by persulfidating MPK4 and increasing the MPK4 kinase activity.
Type of Medium:
Online Resource
ISSN:
2296-889X
DOI:
10.3389/fmolb.2021.635470
DOI:
10.3389/fmolb.2021.635470.s001
DOI:
10.3389/fmolb.2021.635470.s002
DOI:
10.3389/fmolb.2021.635470.s003
DOI:
10.3389/fmolb.2021.635470.s004
DOI:
10.3389/fmolb.2021.635470.s005
Language:
Unknown
Publisher:
Frontiers Media SA
Publication Date:
2021
detail.hit.zdb_id:
2814330-9
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