In:
Evidence-Based Complementary and Alternative Medicine, Hindawi Limited, Vol. 2022 ( 2022-1-28), p. 1-12
Abstract:
Objective. To investigate the mechanism of action of herb-partitioned moxibustion on CD from the perspective of autophagy and immunity. Methods. The expression of microtubule-associated protein LC3II and SQSTM1/p62 in the colon tissues was detected by immunohistochemistry. Western blot was used to detect the expression of autophagic and immune-related proteins in the colon, such as LC3II, SQSTM1/p62, Beclin1, ATG16L1, NOD2, IRGM, IL-1β, IL-17, and TNF-β. mRNA levels of immune factors, such as IL-1β, IL-17, and TNF-β, and autophagy signaling molecules, such as PI3KC, AKT1, LKB1, and mTOR, were detected by RT-qPCR. Results. Herb-partitioned moxibustion reduced the protein levels of ATG16L1, NOD2, IRGM, LC3II, and Beclin1 ( P 〈 0.01 ) and both the protein and mRNA levels of IL-1β, IL-17, and TNF-β in CD rats ( P 〈 0.01 or P 〈 0.05 ), and it also increased the expression of SQSTM1/p62 protein ( P 〈 0.01 ). The modulatory effects of herb-partitioned moxibustion on ATG16L1, NOD2, IRGM, LC3II, TNF-β, and IL-17 protein and IL-1β protein and mRNA were better than those of mesalazine ( P 〈 0.01 or P 〈 0.05 ). Herb-partitioned moxibustion also reduced colon PI3KC, AKT1, and LKB1 mRNA expressions in CD rats ( P 〈 0.01 or P 〈 0.05 ) and increased mTOR protein expression ( P 〈 0.05 ). And the modulatory effect of herb-partitioned moxibustion on AKT1 mRNA was better than that of mesalazine ( P 〈 0.05 ). Conclusion. Herb-partitioned moxibustion may inhibit excessively activated autophagy and modulate the expression of immune-related factors by regulating the LKB1-mTOR-PI3KC signal transduction networks, thereby alleviating intestinal inflammation in CD rats.
Type of Medium:
Online Resource
ISSN:
1741-4288
,
1741-427X
DOI:
10.1155/2022/3534874
Language:
English
Publisher:
Hindawi Limited
Publication Date:
2022
detail.hit.zdb_id:
2148302-4
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