In:
International Journal of Systematic and Evolutionary Microbiology, Microbiology Society, Vol. 65, No. Pt_3 ( 2015-03-01), p. 971-974
Abstract:
A psychrotolerant, Gram-stain-negative, motile, aerobic, peritrichous bacterium, strain DJC1-1 T , was isolated from Lake Dajiaco, Tibetan Plateau, China. The strain was negative for citrate utilization, lipase activity and α-glucosidase, but positive for the Voges–Proskauer reaction and N -acetyl-β-glucosaminidase. 16S rRNA gene sequence analysis indicated that Hafnia paralvei ATCC 29927 T , Hafnia alvei ATCC 13337 T , Serratia grimesii DSM 30063 T and Serratia plymuthica DSM 4540 T were the closest relatives of strain DJC1-1 T , with similarities of 97.76, 96.80, 97.71 and 97.58 %, respectively. The DNA G+C content of strain DJC1-1 T was 53.9 mol%. The predominant fatty acids were C 16 : 0 and C 17 : 0 cyclo. Based on these characteristics, strain DJC1-1 T can be assigned to the genus Hafnia . In DNA–DNA hybridization tests, strain DJC1-1 T shared 50.6, 35.1, 36.5 and 18.1 % DNA–DNA relatedness with the type strains of H. paralvei , H. alvei , S. grimesii and S. plymuthica , respectively. The growth temperature ranged from 0 to 40 °C, with optimum growth at 15 °C. Physiological and biochemical tests differentiated strain DJC1-1 T from the type strains of recognized species of the genus Hafnia . Therefore, strain DJC1-1 T is identified as representing a novel species of the genus Hafnia , for which the name Hafnia psychrotolerans sp. nov. is proposed. The type strain is DJC1-1 T ( = JCM 30077 T = CGMCC1.12806 T ).
Type of Medium:
Online Resource
ISSN:
1466-5026
,
1466-5034
DOI:
10.1099/ijs.0.000049
Language:
English
Publisher:
Microbiology Society
Publication Date:
2015
detail.hit.zdb_id:
215062-1
detail.hit.zdb_id:
2056611-6
SSG:
12
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