In:
Annals of Nutrition and Metabolism, S. Karger AG, Vol. 54, No. 4 ( 2009), p. 253-257
Abstract:
〈 i 〉 Background/Aims: 〈 /i 〉 This study aimed to investigate the quantitative and qualitative changes of bacteria, 〈 i 〉 Bacteroides 〈 /i 〉 , 〈 i 〉 Bifidobacterium 〈 /i 〉 and 〈 i 〉 Clostridium 〈 /i 〉 cluster IV in faecal microbiota associated with a vegetarian diet. 〈 i 〉 Methods: 〈 /i 〉 Bacterial abundances were measured in faecal samples of 15 vegetarians and 14 omnivores using quantitative PCR. Diversity was assessed with PCR-DGGE fingerprinting, principal component analysis (PCA) and Shannon diversity index. 〈 i 〉 Results: 〈 /i 〉 Vegetarians had a 12% higher abundance of bacterial DNA than omnivores, a tendency for less 〈 i 〉 Clostridium 〈 /i 〉 cluster IV (31.86 ± 17.00%; 36.64 ± 14.22%) and higher abundance of 〈 i 〉 Bacteroides 〈 /i 〉 (23.93 ± 10.35%; 21.26 ± 8.05%), which were not significant due to high interindividual variations. PCA suggested a grouping of bacteria and members of 〈 i 〉 Clostridium 〈 /i 〉 cluster IV. Two bands appeared significantly more frequently in omnivores than in vegetarians (p 〈 0.005 and p 〈 0.022). One was identified as 〈 i 〉 Faecalibacterium 〈 /i 〉 sp. and the other was 97.9% similar to the uncultured gut bacteriumDQ793301. 〈 i 〉 Conclusions: 〈 /i 〉 A vegetarian diet affects the intestinal microbiota, especially by decreasing the amount and changing the diversity of 〈 i 〉 Clostridium 〈 /i 〉 cluster IV. It remains to be determined how these shifts might affect the host metabolism and disease risks.
Type of Medium:
Online Resource
ISSN:
0250-6807
,
1421-9697
Language:
English
Publisher:
S. Karger AG
Publication Date:
2009
detail.hit.zdb_id:
1481977-6
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