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  • 1
    Online Resource
    Online Resource
    Table_2.docx
    Frontiers Media SA ; 2023
    In:  Frontiers in Cellular and Infection Microbiology Vol. 12 ( 2023-1-9)
    Details
    In: Frontiers in Cellular and Infection Microbiology, Frontiers Media SA, Vol. 12 ( 2023-1-9)
    Abstract: The intrinsic resistance mechanism plays an essential role in the bacterial resistance to a variety of the antimicrobials. The aim of this study is to find the chromosome-encoded novel antimicrobial resistance gene in the clinical isolate. Methods The function of the predicted resistance gene was verified by gene cloning and antibiotic susceptibility test. Recombinant protein expression and enzyme kinetic studies were performed to explore the in vivo activity of the enzyme. Expression of the resistance gene exposed to antimicrobial was determined by RT-qPCR. Whole genome sequencing and bioinformatic analysis were applied to analyze the genetic context of the resistance gene. Results The novel aminoglycoside (AG) resistance genes designated aph(9)-Ic and aph(9)-Ic1 confer resistance to spectinomycin, and a recombinant strain harboring aph(9)-Ic (pMD19-T-aph(9)-Ic/DH5α) showed a significantly increased minimum inhibitory concentration (MIC) level against spectinomycin compared with the control strains (DH5α and pMD19-T/DH5α). The result of the kinetic analysis of APH(9)-Ic was consistent with the MIC result for the recombinant pMD19-T-aph(9)-Ic/DH5α, showing the efficient catalytic activity for spectinomycin [kcat/Km ratio = (5.58 ± 0.31) × 104 M−1·s−1]. Whole-genome sequencing demonstrated that the aph(9)-Ic gene was located on the chromosome with a relatively conserved genetic environment, and no mobile genetic element was found in its surrounding region. Among all the function-characterized resistance genes, APH(9)-Ic shares the highest amino acid sequence identity of 33.75% with APH(9)-Ia. Conclusion We characterized a novel AG resistance gene aph(9)-Ic and its variant aph(9)-Ic1 that mediated spectinomycin resistance from S. maltophilia. The identification of the novel AG resistance genes will assist us in elucidating the complexity of resistance mechanisms in microbial populations.
    Type of Medium: Online Resource
    ISSN: 2235-2988
    URL: Article
    URL: Article
    URL: Article
    URL: Article
    URL: Article
    URL: Article
    URL: Article
    DOI: 10.3389/fcimb.2022.1097561
    DOI: 10.3389/fcimb.2022.1097561.s001
    DOI: 10.3389/fcimb.2022.1097561.s002
    DOI: 10.3389/fcimb.2022.1097561.s003
    DOI: 10.3389/fcimb.2022.1097561.s004
    DOI: 10.3389/fcimb.2022.1097561.s005
    DOI: 10.3389/fcimb.2022.1097561.s006
    Language: Unknown
    Publisher: Frontiers Media SA
    Publication Date: 2023
    detail.hit.zdb_id: 2619676-1
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  • 2
    Online Resource
    Online Resource
    The Value of CT-Guided Percutaneous Lung Biopsy in the Diagnosis of Pulmonary Fungal Infections in Patients with Hematologic Diseases.
    American Society of Hematology ; 2007
    In:  Blood Vol. 110, No. 11 ( 2007-11-16), p. 3860-3860
    Details
    In: Blood, American Society of Hematology, Vol. 110, No. 11 ( 2007-11-16), p. 3860-3860
    Abstract: Introduction: Patients with hematologic diseases are at high risk for invasive fungal infections (IFI).The diagnosis of IFI is difficult with specificity as low as 20%–30%. The currently used diagnostic methods for IFI such as CT scan, fungal cultures, and antigen detection are insufficient in reaching a definitive diagnosis. Histopathology with tissue diagnosis is the method of choice. CT-guided percutaneous lung biopsy (CT-PCLB) can provide pathological diagnosis and hence significant guidance for clinical management. Objectives: To evaluate the sensitivity and safety of CT-PCLB for the diagnosis of pulmonary fungal infections in patients with hematologic diseases. Patients and Method: We retrospectively studied 16 patients hospitalized in our department from April 2005 to December 2006 who were clinically regarded as having pulmonary fungal infections by clinical symptoms and imaging and who subsequently underwent CT-PCLB. Among the 16 patients, 10 had AML, 2 had ALL, 2 had ITP, 1 had MDS and 1 had multiple myeloma. At the time of percutaneous lung biopsies, median WBC was 3.2 k/ul (range 0.7–7.2 k/ul), median platelets were 82 k/ul (range 32–453 k/ul). Peripheral blood cultures were performed in all patients. Eight patients who had symptoms of cough and expectoration also received sputum culture for at least three times. Of the 16 patients, 10 were diagnosed with fungal infections (8 aspergillus’s and 2 mold fungi), 4 with chronic organizing pneumonitis, 1 with tuberculosis, and 1 with pneumocystis carinii. All tissue diagnoses were from histological examination with samples obtained from CT-PCLB. However, the results of blood cultures and sputum cultures were negative in these 8 patients. CT-PCLB diagnosed 10 out of 16 patients for a fungal infection rate of 63%. The biopsy-induced complications encountered were pneumothorax in 3/16 (19%) and hemoptysis in 6% (1/16). No surgical interventions were needed for the 3 patients with pneumothoraxes that resolved spontaneous under close observation. Antifungal agents (such as voriconazole) had been given to 8 patients diagnosed as aspergillus infection and all patients had a complete or partial response by CT imaging. However, 2 patients who received broad-spectrum antibacterial antibiotics showed clinical improvement, which indicated possible false negative diagnoses for fungal infections by CT-PCLB. Conclusion: CT-guided percutaneous lung biopsy (CT-PCLB) was a safe, sensitive and specific method for the diagnosis of pulmonary fungal infections in patients with hematologic diseases. Its definitive value should be tested in a prospective clinical trial.
    Type of Medium: Online Resource
    ISSN: 0006-4971 , 1528-0020
    URL: Article
    DOI: 10.1182/blood.V110.11.3860.3860
    RVK:
    XA 33000
    RVK:
    XA 10000
    Language: English
    Publisher: American Society of Hematology
    Publication Date: 2007
    detail.hit.zdb_id: 1468538-3
    detail.hit.zdb_id: 80069-7
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  • 3
    Online Resource
    Online Resource
    High Expression of Mannose-Binding Lectin and the Risk of Vascular Complications of Diabetes: Evidence from a Meta-Analysis
    Mary Ann Liebert Inc ; 2015
    In:  Diabetes Technology & Therapeutics Vol. 17, No. 7 ( 2015-07), p. 490-497
    Details
    In: Diabetes Technology & Therapeutics, Mary Ann Liebert Inc, Vol. 17, No. 7 ( 2015-07), p. 490-497
    Type of Medium: Online Resource
    ISSN: 1520-9156 , 1557-8593
    URL: Article
    DOI: 10.1089/dia.2014.0372
    Language: English
    Publisher: Mary Ann Liebert Inc
    Publication Date: 2015
    detail.hit.zdb_id: 2004914-6
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  • 4
    Online Resource
    Online Resource
    Association of induced abortion with preterm birth risk in first-time mothers
    Springer Science and Business Media LLC ; 2018
    In:  Scientific Reports Vol. 8, No. 1 ( 2018-03-29)
    Details
    In: Scientific Reports, Springer Science and Business Media LLC, Vol. 8, No. 1 ( 2018-03-29)
    Abstract: Women who have previously had an induced abortion (IA) before their first birth have been associated with preterm birth (PTB). However, previous studies on the PTB are inconsistent. Therefore, the aim of this study was to clarify the association between IA and PTB and low birth weight (LBW) for first-time mothers. A total of 3,684 Southern Chinese women who gave birth for the first time to a live singleton infants were recruited between January 2015 and December 2015 in the province of Guangdong, China. Univariable and multivariable analyses were conducted to determine whether IA was associated with PTB and LBW. Previous IA was not associated with increased risks of PTB or LBW, adjusted odds ratios were 0.80 (95% CI = 0.53 to 1.20) and 0.86 (95% CI = 0.57 to 1.31), respectively. Additionally, no significant associations were observed for infants born at before 37, before 32, and before 28 gestational weeks. And no significant associations were also observed for LBW measuring lower than 2500 grams and also measuring lower than 1500 grams. Our study suggested that a previous IA, as compared with women who reported no previous IA, does not increase the risk of PTB or LBW in subsequent pregnancy for the first-time mothers among Southern Chinese women.
    Type of Medium: Online Resource
    ISSN: 2045-2322
    URL: Article
    DOI: 10.1038/s41598-018-23695-7
    Language: English
    Publisher: Springer Science and Business Media LLC
    Publication Date: 2018
    detail.hit.zdb_id: 2615211-3
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  • 5
    Online Resource
    Online Resource
    Combination Therapy Based on Bortezomib for Newly Diagnosed Multiple Myeloma Patients
    American Society of Hematology ; 2012
    In:  Blood Vol. 120, No. 21 ( 2012-11-16), p. 5048-5048
    Details
    In: Blood, American Society of Hematology, Vol. 120, No. 21 ( 2012-11-16), p. 5048-5048
    Abstract: Abstract 5048 Introduction: Novel drugs, such as bortezomib, have significantly improved the response rates in multiple myeloma (MM), but little has been reported on bortezomib-based therapies in Chinese patients. Methods: In the initial eight 28-day cycles, newly diagnosed ymptomatic patients were treated with combination therapy including bortezomib plus dexamethasone (PD) and the triplet combinations of PD with adriamycin (PAD), cyclophosphamide (PCD), thalidomide (PDT) between February 1, 2006 and May 31, 2012. Among the above regimens, bortezomib (1. 3 mg/m2) was given intravenously on days 1, 4, 8, 11, while dexamethasone (20 mg/m2/day) was given intravenously on days 1–2, 4–5, 8–9, 11–12, adriamycin (10 mg/m2) was given intravenously on days 1–4, cyclophosphamide (200 mg/m2) was given intravenously on days 1–4 and thalidomide (100 mg) was administered orally each day. Results: The overall response rate (¡Ý partial response, PR) of all the 151 eligible patients was 88. 7% (including 29. 8% very good partial response (VGPR) and 25. 8% complete response/near complete response (CR/nCR). The responses per IMWG criteria for patients are shown in Table 2. The median PFS was 20. 3 months (95% CI: 14. 8–25. 8 months) in the patients who received PDT, 24. 8 months (95% CI: 20. 0–30. 0 months) in the patients who received PCD, 22. 9 months (95% CI: 17. 6–28. 2 months) in patients who received PAD and 21. 8 months (95% CI: 15. 3–28. 3 months) in the patients who received PD with no significant differences between the groups. The median OS for PD arm was 42. 0(95% CI: 20. 1–63. 9 months) months while other arms were not reached, but the median OS for PDT, PCD and PAD was significant longer than PD (P=0. 042, 0. 039, 0. 010). PFS and OS for patients with favorable cytogenetics were significantly longer than those with unfavorable cytogenetics by FISH. The frequently observed hematologic toxicities (Grade 3/4) were: thrombocytopenia (17. 00%), neutropenia (15. 00%) and anemia (8. 61%). The most common non-hematologic toxicities included (all Grades) peripheral neuropathy(57. 61%), fatigue(27. 15%), infection(23. 84%), constipation(22. 52%), herpes zoster(17. 22%) and diarrhea(15. 23%). Conclusions: Our experience indicated that bortezomib-based regimens were active and well-tolerated for MM patients, and triplet combinations were superior to PD. Serious Adverse events were rare in the Chinese patients with MM who received bortezomib-based chemotherapy. Disclosures: No relevant conflicts of interest to declare.
    Type of Medium: Online Resource
    ISSN: 0006-4971 , 1528-0020
    URL: Article
    DOI: 10.1182/blood.V120.21.5048.5048
    RVK:
    XA 33000
    RVK:
    XA 10000
    Language: English
    Publisher: American Society of Hematology
    Publication Date: 2012
    detail.hit.zdb_id: 1468538-3
    detail.hit.zdb_id: 80069-7
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  • 6
    Online Resource
    Online Resource
    Image_1.JPEG
    Frontiers Media SA ; 2022
    In:  Frontiers in Microbiology Vol. 13 ( 2022-9-13)
    Details
    In: Frontiers in Microbiology, Frontiers Media SA, Vol. 13 ( 2022-9-13)
    Abstract: A novel chromosome-encoded aminoglycoside O -nucleotidyltransferase AadA33 was identified in Providencia vermicola strain P13. The AadA33 shares the highest amino acid identity of 51.28% with the function characterized AadA31. Antibiotic susceptibility testing and enzyme kinetics analysis revealed that the function of AadA33 is to mediate spectinomycin and streptomycin resistance. The recombinant strain harboring aadA33 (pUCP20- aadA33 / Escherichia coli DH5α) displayed & gt;256- and 128-fold increases in the minimum inhibitory concentration levels to spectinomycin and streptomycin, respectively, compared with the control strains pUCP20/DH5α. Enzyme kinetic parameters manifested the substrate of AadA33 including spectinomycin and streptomycin, with k cat / K m of 3.28 × 10 4 (M −1 s −1 ) and 3.37 × 10 4 (M −1 s −1 ), respectively. Bioinformatics analysis revealed its structural mechanism of antimicrobial resistance, genetic context, and phylogenetic relationship with other aminoglycoside O -nucleotidyltransferases. This study of AadA33 contributed to understanding the function and resistance mechanism of aminoglycoside O -nucleotidyltransferase.
    Type of Medium: Online Resource
    ISSN: 1664-302X
    URL: Article
    URL: Article
    DOI: 10.3389/fmicb.2022.990739
    DOI: 10.3389/fmicb.2022.990739.s001
    Language: Unknown
    Publisher: Frontiers Media SA
    Publication Date: 2022
    detail.hit.zdb_id: 2587354-4
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  • 7
    Online Resource
    Online Resource
    Supplemental Information 1: Raw data
    PeerJ ; 2020
    In:  PeerJ Vol. 8 ( 2020-10-14), p. e10123-
    Details
    In: PeerJ, PeerJ, Vol. 8 ( 2020-10-14), p. e10123-
    Abstract: Studies have reported an increased risk of adverse pregnancy outcome associated with pre-pregnancy body mass index (BMI). However, the data on such associations in urban areas of southern Chinese women is limited, which drive us to clarify the associations of pre-pregnancy BMI and the risks of adverse pregnancy outcomes (preterm birth (PTB) and low birth weight (LBW)) and maternal health outcomes (gestational hypertension and cesarean delivery). Methods We performed a hospital-based case-control study including 3,864 Southern Chinese women who gave first birth to a live singleton infant from January 2015 to December 2015. PTB was stratified into three subgroups according to gestational age (extremely PTB, very PTB and moderate PTB). Besides, we combined birth weight and gestational age to dichotomise as being small for gestational age (SGA, less than the tenth percentile of weight for gestation) and non-small for gestational age (NSGA, large than the tenth percentile of weight for gestation), gestational week was also classified into categories of term, 34-36 week and below 34 week.. We then divided newborns into six groups: (1) term and NSGA; (2) 34–36 week gestation and NSGA; (3) below 34 week gestation and NSGA; (4) term and SAG; (5) 34–36 week gestation and SAG; (6) below 34 week gestation and SAG. Adjusted logistic regression models was used to estimate the odds ratios of adverse outcomes. Results Underweight women were more likely to give LBW (AOR = 1.44, 95% CI [1.11–1.89]), the similar result was seen in term and SAG as compared with term and NSAG (AOR = 1.78, 95% CI [1.45–2.17] ), whereas underweight was significantly associated with a lower risk of gestational hypertension (AOR = 0.45, 95% CI [0.25–0.82) and caesarean delivery (AOR = 0.74, 95% CI [0.62–0.90]). The risk of extremely PTB is relatively higher among overweight and obese mothers in a subgroup analysis of PTB (AOR = 8.12, 95% CI [1.11–59.44] ; AOR = 15.06, 95% CI [1.32–172.13], respectively). Both maternal overweight and obesity were associated with a greater risk of gestational hypertension (AOR = 1.71, 95% CI [1.06–2.77] ; AOR = 5.54, 95% CI [3.02–10.17], respectively) and caesarean delivery (AOR = 1.91, 95% CI [1.53–2.38] ; AOR = 1.85, 95% CI [1.21–2.82], respectively). Conclusions Our study suggested that maternal overweight and obesity were associated with a significantly higher risk of gestational hypertension, caesarean delivery and extremely PTB. Underweight was correlated with an increased risk of LBW and conferred a protective effect regarding the risk for gestational hypertension and caesarean delivery for the first-time mothers among Southern Chinese.
    Type of Medium: Online Resource
    ISSN: 2167-8359
    URL: Article
    URL: Article
    URL: Article
    URL: Article
    URL: Article
    URL: Article
    DOI: 10.7717/peerj.10123
    DOI: 10.7717/peerj.10123/table-1
    DOI: 10.7717/peerj.10123/table-2
    DOI: 10.7717/peerj.10123/table-3
    DOI: 10.7717/peerj.10123/table-4
    DOI: 10.7717/peerj.10123/supp-1
    Language: English
    Publisher: PeerJ
    Publication Date: 2020
    detail.hit.zdb_id: 2703241-3
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  • 8
    Online Resource
    Online Resource
    Design, synthesis and biological evaluation of novel tumor hypoxia-activated EGFR tyrosine kinase inhibitors
    Elsevier BV ; 2022
    In:  Bioorganic Chemistry Vol. 129 ( 2022-12), p. 106138-
    Details
    In: Bioorganic Chemistry, Elsevier BV, Vol. 129 ( 2022-12), p. 106138-
    Type of Medium: Online Resource
    ISSN: 0045-2068
    URL: Article
    DOI: 10.1016/j.bioorg.2022.106138
    Language: English
    Publisher: Elsevier BV
    Publication Date: 2022
    detail.hit.zdb_id: 1462232-4
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  • 9
    Online Resource
    Online Resource
    A Retrospective Analysis of Cytogenetic and Clinical Characteristics in Patients With Multiple Myeloma
    Elsevier BV ; 2013
    In:  The American Journal of the Medical Sciences Vol. 345, No. 2 ( 2013-02), p. 88-93
    Details
    In: The American Journal of the Medical Sciences, Elsevier BV, Vol. 345, No. 2 ( 2013-02), p. 88-93
    Type of Medium: Online Resource
    ISSN: 0002-9629
    URL: Article
    DOI: 10.1097/MAJ.0b013e31825b32bc
    Language: English
    Publisher: Elsevier BV
    Publication Date: 2013
    detail.hit.zdb_id: 2083424-X
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  • 10
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    Interferon Consensus Sequence-Binding Protein 8, a Tumor Suppressor, Suppresses Tumor Growth and Invasion of Non-Small Cell Lung Cancer by Interacting with the Wnt/β-Catenin Pathway
    S. Karger AG ; 2018
    In:  Cellular Physiology and Biochemistry Vol. 51, No. 2 ( 2018), p. 961-978
    Details
    In: Cellular Physiology and Biochemistry, S. Karger AG, Vol. 51, No. 2 ( 2018), p. 961-978
    Abstract: Background/Aims: Interferon consensus sequence-binding protein 8 (IRF8) belongs to a family of interferon (IFN) regulatory factors that modulates various important physiological processes including carcinogenesis. As reported by others and our group, IRF8 expression is silenced by DNA methylation in both human solid tumors and hematological malignancies. However, the role of IRF8 in lung carcinoma remains elusive. In this study, we determined IRF8 epigenetic regulation, biological functions, and the signaling pathway involved in non-small cell lung cancer (NSCLC). Methods: IRF8 expression were determined by Q- PCR. MSP and A+T determined promotor methylation. MTS, clonogenic, Transwell assay, Flow cytometry, three-dimensional culture and AO/EB stain verified cell function. In vivo tumorigenesis examed the in vivo effects. By Chip-QPCR, RT-PCR, Western blot and Immunofluorescence staining, the mechanisms were studied. Results: IRF8 was significantly downregulated in lung tumor tissues compared with adjacent non-cancerous tissues. Furthermore, methylation-specific PCR analyses revealed that IRF8 methylation in NSCLC was a common event, and demethylation reagent treatment proved that downregulation of IRF8 was due to its promoter CpG hypermethylation. Clinical data showed that the IRF8 methylation was associated with tumor stage, lymph node metastasis status, patient outcome, and tumor histology. Exogenous expression of IRF8 in the silenced or downregulated lung cancer cell lines A549 and H1299 at least partially restored the sensitivity of lung cancer cells to apoptosis, and arrested cells at the G0/G1 phase. Cell viability, clonogenicity, and cell migration and invasive abilities were strongly inhibited by restored expression of IRF8. A three-dimensional culture system demonstrated that IRF8 changed the cells to a more spherical phenotype. Moreover, ectopic expression of IRF8 enhanced NSCLC chemosensitivity to cisplatin. Furthermore, as verified by Chip-qPCR, immunofluorescence staining, and western blotting, IRF8 bound to the T-cell factor/lymphoid enhancer factor (TCF /LEF) promoter, thus repressing β-catenin nuclear translocation and its activation. IRF8 significantly disrupted the effects of Wnt agonist, bml284, further suggesting its involvement in the Wnt/β-catenin pathway. Conclusion: IRF8 acted as a tumor suppressor gene through the transcriptional repression of β-catenin-TCF/LEF in NSCLC. IRF8 methylation may serve as a potential biomarker in NSCLC prognosis.
    Type of Medium: Online Resource
    ISSN: 1015-8987 , 1421-9778
    URL: Article
    DOI: 10.1159/000495399
    Language: English
    Publisher: S. Karger AG
    Publication Date: 2018
    detail.hit.zdb_id: 1482056-0
    SSG: 12
    SSG: 15,3
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