In:
Frontiers in Plant Science, Frontiers Media SA, Vol. 12 ( 2021-5-25)
Abstract:
Hypersensitive response (HR)-like cell death is an important mechanism that mediates the plant response to pathogens. In our previous study, we reported that NbHIR3s regulate HR-like cell death and basal immunity. However, the host genes involved in HR have rarely been studied. Here, we used transcriptome sequencing to identify Niben101Scf02063g02012.1 , an HR-like lesion inducing protein (HRLI) in Nicotiana benthamiana that was significantly reduced by turnip mosaic virus (TuMV). HRLIs are uncharacterized proteins which may regulate the HR process. We identified all six HRLIs in N. benthamiana and functionally analyzed Niben101Scf02063g02012.1 , named NbHRLI4 , in response to TuMV. Silencing of NbHRLI4 increased TuMV accumulation, while overexpression of NbHRLI4 conferred resistance to TuMV. Transient overexpression of NbHRLI4 caused cell death with an increase in the expression of salicylic acid (SA) pathway genes but led to less cell death level and weaker immunity in plants expressing NahG . Thus, we have characterized NbHRLI4 as an inducer of cell death and an antiviral regulator of TuMV infection in a SA-mediated manner.
Type of Medium:
Online Resource
ISSN:
1664-462X
DOI:
10.3389/fpls.2021.627315
DOI:
10.3389/fpls.2021.627315.s001
DOI:
10.3389/fpls.2021.627315.s002
DOI:
10.3389/fpls.2021.627315.s003
DOI:
10.3389/fpls.2021.627315.s004
DOI:
10.3389/fpls.2021.627315.s005
DOI:
10.3389/fpls.2021.627315.s006
DOI:
10.3389/fpls.2021.627315.s007
DOI:
10.3389/fpls.2021.627315.s008
DOI:
10.3389/fpls.2021.627315.s009
DOI:
10.3389/fpls.2021.627315.s010
DOI:
10.3389/fpls.2021.627315.s011
DOI:
10.3389/fpls.2021.627315.s012
DOI:
10.3389/fpls.2021.627315.s013
DOI:
10.3389/fpls.2021.627315.s014
Language:
Unknown
Publisher:
Frontiers Media SA
Publication Date:
2021
detail.hit.zdb_id:
2687947-5
detail.hit.zdb_id:
2613694-6
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