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  • 1
    Online Resource
    Online Resource
    Springer Science and Business Media LLC ; 2024
    In:  Applied Microbiology and Biotechnology Vol. 108, No. 1 ( 2024-12)
    In: Applied Microbiology and Biotechnology, Springer Science and Business Media LLC, Vol. 108, No. 1 ( 2024-12)
    Abstract: A more optimized culture medium used in vitro to mimic the bacterial composition of original oral flora as similar as possible remains difficult at present, and the goal of this study is to develop a novel oral biofilm medium to restore the original oral microbiome. Firstly, we conducted a s ystematic literature review by searching PubMed and summarized the current reported culture media in vitro . Seven culture media were found. We used mixed saliva as the origin of oral species to compare the effects of the above media in culturing oral multispecies biofilms. Results indicated that among the seven media brain heart infusion containing 1% sucrose (BHIs) medium, PG medium, artificial saliva (AS) medium, and SHI medium could obviously gain large oral biofilm in vitro. The nutrients contained in different culture media may be suitable for the growth of different oral bacteria; therefore, we optimized several novel media accordingly. Notably, results of crystal violet staining showed that the biofilm cultured in our modified artificial saliva (MAS) medium had the highest amount of biofilm biomass. 16S rRNA gene sequencing showed that the operational taxonomic units (OTUs) and Shannon index of biofilm cultured in MAS medium were also the highest among all the tested media. More importantly, the 16S rRNA gene sequencing analysis indicated that the biofilm cultured in MAS medium was closer to the original saliva species. Besides, biofilm cultured by MAS was denser and produced more exopolysaccharides. MAS supported stable biofilm formation on different substrata. In conclusion, this study demonstrated a novel MAS medium that could culture oral biofilm in vitro closer to the original oral microbiome, showing a good application prospect. Key points • We compare the effects of different media in culturing oral biofilms • A novel modified artificial saliva (MAS) medium was obtained in our study • The MAS medium could culture biofilm that was closer to oral microbiome
    Type of Medium: Online Resource
    ISSN: 0175-7598 , 1432-0614
    RVK:
    Language: English
    Publisher: Springer Science and Business Media LLC
    Publication Date: 2024
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  • 2
    Online Resource
    Online Resource
    Elsevier BV ; 2023
    In:  Journal of Dentistry Vol. 135 ( 2023-08), p. 104596-
    In: Journal of Dentistry, Elsevier BV, Vol. 135 ( 2023-08), p. 104596-
    Type of Medium: Online Resource
    ISSN: 0300-5712
    Language: English
    Publisher: Elsevier BV
    Publication Date: 2023
    detail.hit.zdb_id: 186068-9
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  • 3
    Online Resource
    Online Resource
    Elsevier BV ; 2024
    In:  Chemical Engineering Journal Vol. 497 ( 2024-10), p. 154458-
    In: Chemical Engineering Journal, Elsevier BV, Vol. 497 ( 2024-10), p. 154458-
    Type of Medium: Online Resource
    ISSN: 1385-8947
    Language: English
    Publisher: Elsevier BV
    Publication Date: 2024
    detail.hit.zdb_id: 241367-X
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  • 4
    In: Chemical Engineering Journal, Elsevier BV, ( 2024-8), p. 154518-
    Type of Medium: Online Resource
    ISSN: 1385-8947
    Language: English
    Publisher: Elsevier BV
    Publication Date: 2024
    detail.hit.zdb_id: 241367-X
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  • 5
    In: Community Dentistry and Oral Epidemiology, Wiley, Vol. 49, No. 1 ( 2021-02), p. 47-54
    Abstract: This study aimed to measure socioeconomic‐related inequality and horizontal inequity in the use of oral health services and decompose this inequality among adults of different age groups in China. Methods In total, 10 973 adults (3669 aged 35‐44 years, 3767 aged 55‐64 years and 3537 aged 65‐74 years) who participated in the 4th National Oral Health Survey (2015‐2016) in China were included. Concentration curves and the Erreygers‐corrected concentration index (EI) were employed to measure socioeconomic‐related inequality in the use of oral health services. Then, inequity in this utilization was measured by the horizontal inequity index (HI). Furthermore, decomposition analyses were conducted for the three groups to explain the contributions of income level, need factors (ie self‐assessed oral health and evaluated oral health status), other factors (ie sex, residential location, educational attainment level and type of basic insurance) and a residual term to overall inequality in oral health service utilization. Results The significant positive EI and HI values indicated that pro‐rich inequality and inequity in oral health service utilization exist among Chinese adults. Income and type of basic medical insurance contributed the most to socioeconomic‐related inequality in the use of oral health services among adults aged 55‐64 and 65‐74 years. However, the main driving factors of socioeconomic inequality among adults aged 35‐44 years in dental care use included income, educational achievement, type of basic medical insurance and residential location. The need variables accounted for a very small proportion of overall socioeconomic‐related inequality in oral health service use in all three groups. Conclusions Oral healthcare service utilization was disproportionately concentrated among better‐off Chinese adults. The primary determinants of inequality in dental care use in different age groups provide information for policymakers to create more targeted policies to achieve equity in the oral healthcare system in China.
    Type of Medium: Online Resource
    ISSN: 0301-5661 , 1600-0528
    URL: Issue
    Language: English
    Publisher: Wiley
    Publication Date: 2021
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  • 6
    Online Resource
    Online Resource
    S. Karger AG ; 2018
    In:  Caries Research Vol. 52, No. 3 ( 2018), p. 230-245
    In: Caries Research, S. Karger AG, Vol. 52, No. 3 ( 2018), p. 230-245
    Abstract: Research on the association between dental caries and body mass index (BMI) in children has shown contradictory results; thus we aimed to examine the association between dental caries and the full range of BMI classes among children. We comprehensively searched PubMed, Embase, and the Cochrane Library for studies published prior to March 2017. Articles comparing dental caries among the full range of BMI classes for children below 18 years of both genders were included. Fourteen studies were eligible for this study. Basic information - i.e., first author, published year, study design, country, sample size, age, type of dental caries index and BMI, main results and conclusions, and means and standard deviations of the dental caries indexes used - was pooled. The weighted mean differences and corresponding 95% confidence intervals for dental caries between children with abnormal weight and those with normal weight were analyzed. Generally, no significant differences in caries were found between any abnormal-weight group and the normal-weight group for both primary and permanent teeth. Sensitivity analyses showed that the obese group had more caries than the normal-weight group in their primary teeth. Significantly more caries was found among the overweight and obese children in both primary and permanent teeth in high-income countries, but not in low- and middle-income countries. We recommend that further studies use suitable sample sizes, unify the criteria for BMI categorization and the dental caries index, and investigate the confounding factors that might influence dental caries and BMI.
    Type of Medium: Online Resource
    ISSN: 0008-6568 , 1421-976X
    RVK:
    Language: English
    Publisher: S. Karger AG
    Publication Date: 2018
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  • 7
    Online Resource
    Online Resource
    Wiley ; 2023
    In:  International Journal of Dental Hygiene Vol. 21, No. 3 ( 2023-08), p. 599-610
    In: International Journal of Dental Hygiene, Wiley, Vol. 21, No. 3 ( 2023-08), p. 599-610
    Abstract: Studies on the association between adverse birth outcomes and dental caries in children have shown conflicting results, so the aim of this systematic review and meta‐analysis was to investigate the association between adverse birth outcomes and dental caries in children. Methods We systematically searched articles in four electronic databases (Web of Science, PubMed, Cochrane Library and Embase) published prior to August 2021. The odds ratio (OR) (or converted OR) and the corresponding 95% confidence intervals (95% CI) were processed. The certainty of evidence was assessed using GRADE's risk bias assessment tool. Random effects model was used for this meta‐analysis. Results A total of thirty‐one observational studies met the inclusion criteria. The pooled estimates indicated that children exposed to low birth weight (LBW)/preterm birth (PTB) did not experience higher dental caries in primary teeth. Subgroup analyses showed that children with LBW (OR: 1.30, 95% CI: 1.03–1.63) were prone to have dental caries in primary teeth for cross‐sectional studies, but no significant differences for prospective studies. PTB children experienced more primary caries in high‐income countries (OR: 1.31, 95% CI: 1.00–1.70) than in low‐ and middle‐income countries. Conclusions The current evidence did not suggest a significant association between LBW and dental caries in children for primary teeth. Children with PTB in high‐income countries had a higher prevalence of primary dental caries. Further prospective studies should adjust for confounding factors (age, oral health and family finances) to determine the definitive association between LBW/PTB and dental caries.
    Type of Medium: Online Resource
    ISSN: 1601-5029 , 1601-5037
    URL: Issue
    Language: English
    Publisher: Wiley
    Publication Date: 2023
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    detail.hit.zdb_id: 2252118-5
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  • 8
    Online Resource
    Online Resource
    Springer Science and Business Media LLC ; 2021
    In:  Arthritis Research & Therapy Vol. 23, No. 1 ( 2021-12)
    In: Arthritis Research & Therapy, Springer Science and Business Media LLC, Vol. 23, No. 1 ( 2021-12)
    Abstract: Isopsoralen (IPRN), one of the active ingredients of Psoralea corylifolia Linn, has anti-inflammatory properties. We attempted to investigate the inhibitory effects of IPRN on rheumatoid arthritis (RA) and characterize its potential mechanism. Methods RA fibroblast-like synoviocytes (FLSs) and mice with collagen-induced arthritis (CIA) were used as in vitro and in vivo models to analyze the antiarthritic effect of IPRN. Histological analysis of the inflamed joints from mice with CIA was performed using microcomputed tomography (micro-CT) and hematoxylin-eosin (HE) staining. RNA sequencing (RNA-Seq), network pharmacology analysis, molecular docking, drug affinity responsive target stability (DARTS) assay, and cellular thermal shift assay (CETSA) were performed to evaluate the targets of IPRN. Results IPRN ameliorated the inflammatory phenotype of RA FLSs by inhibiting their cytokine production, migration, invasion, and proangiogenic ability. IPRN also significantly reduced the severity of CIA in mice by decreasing paw thickness, arthritis score, bone damage, and serum inflammatory cytokine levels. A mechanistic study demonstrated that macrophage migration inhibitory factor (MIF), a key protein in the inflammatory process, was the specific target by which IPRN exerted its anti-inflammatory effects in RA FLSs. Conclusion Our study demonstrates the antiarthritic effect of IPRN, which suggests the therapeutic potential of IPRN in RA.
    Type of Medium: Online Resource
    ISSN: 1478-6362
    Language: English
    Publisher: Springer Science and Business Media LLC
    Publication Date: 2021
    detail.hit.zdb_id: 2041668-4
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  • 9
    Online Resource
    Online Resource
    Frontiers Media SA ; 2023
    In:  Frontiers in Microbiology Vol. 14 ( 2023-4-4)
    In: Frontiers in Microbiology, Frontiers Media SA, Vol. 14 ( 2023-4-4)
    Type of Medium: Online Resource
    ISSN: 1664-302X
    Language: Unknown
    Publisher: Frontiers Media SA
    Publication Date: 2023
    detail.hit.zdb_id: 2587354-4
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  • 10
    Online Resource
    Online Resource
    Frontiers Media SA ; 2020
    In:  Frontiers in Pediatrics Vol. 8 ( 2020-7-28)
    In: Frontiers in Pediatrics, Frontiers Media SA, Vol. 8 ( 2020-7-28)
    Type of Medium: Online Resource
    ISSN: 2296-2360
    Language: Unknown
    Publisher: Frontiers Media SA
    Publication Date: 2020
    detail.hit.zdb_id: 2711999-3
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