In:
Cancer Research, American Association for Cancer Research (AACR), Vol. 76, No. 14_Supplement ( 2016-07-15), p. 1977-1977
Abstract:
EZR, a member of the ezrin-radixin-moesin (ERM) family, is involved in multiple aspects of cell migration by acting both as crosslinkers between the membrane, receptors and the actin cytoskeleton, and as regulators of signalling molecules that are implicated in cell adhesion, cell polarity and migration. SMYD3 (SET and MYND Domain-containing protein 3), an H3K4 histone methyltransferase, plays important roles in EZR gene transcription activity. However, the molecular mechanisms of how to recruit this epigenetic modifier to the EZR locus still remain ill-defined. In this study, we identify a natural antisense transcript (NAT) EZR antisense AS1 (EZR-AS1), which is transcribed from the opposite strand on the EZR gene locus, is involved in the SMYD3-mediated transcription of EZR. We find that EZR-AS1 is expressed in esophageal squamous cell carcinoma (ESCC) cells and is positive correlated with EZR expression in both ESCC cells and human ESCC tissues. Silenced EZR-AS1expression decreased EZR expression at both RNA and protein levels, resulting in an apparent inhibitory effect on cell motility. Overexpression of EZR in the EZR-AS1 depleted cells partly restored the mobility of cells. Through RNA-binding protein immunoprecipitation (RIP) assay and RNA pull down assay, we further demonstrate EZR-AS1 directly interacts with SMYD3, and it depends on an RNA/DNA hybrid formation. EZR-AS1 knockdown led to a significant decrease in both SMYD3 occupancy and histone H3K4me3 at the EZR promoter region, whereas EZR-AS1 overexpression results the opposite effects. Together, our results suggest a novel mechanism of interaction between a NAT EZR-AS1 and SMYD3 drives the EZR transcription in ESCC cells. Citation Format: Xiaodan Zhang, Guo-Wei Huang, Lian-Di Liao, Lin Long, En-Min Liao, Li-Yan Xu. Interaction between long noncoding RNA EZR-AS1 and SMYD3 promotes EZR expression in ESCC cells. [abstract]. In: Proceedings of the 107th Annual Meeting of the American Association for Cancer Research; 2016 Apr 16-20; New Orleans, LA. Philadelphia (PA): AACR; Cancer Res 2016;76(14 Suppl):Abstract nr 1977.
Type of Medium:
Online Resource
ISSN:
0008-5472
,
1538-7445
DOI:
10.1158/1538-7445.AM2016-1977
Language:
English
Publisher:
American Association for Cancer Research (AACR)
Publication Date:
2016
detail.hit.zdb_id:
2036785-5
detail.hit.zdb_id:
1432-1
detail.hit.zdb_id:
410466-3
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