In:
Science Advances, American Association for the Advancement of Science (AAAS), Vol. 6, No. 43 ( 2020-10-23)
Abstract:
Directly observing intracellular nanostructure formation remains challenging. In this work, using a rationally designed small-molecule 4-nitrobenzyl carbamate–Cys(SEt)-Asp-Asp-Phe(iodine)–2-cyano-benzothiazole (NBC-Iod-CBT), we directly observed intracellular nanoparticle formation with nanocomputed tomography (nano-CT). In vitro, upon glutathione reduction and nitroreductase (NTR) cleavage, NBC-Iod-CBT undergoes a CBT-Cys click condensation reaction to self-assemble nanoparticles Iod-CBT-NPs with an average linear absorption coefficient (LAC) value of 0.182 ± 0.078 μm −1 to x-ray. Nano-CT imaging of the NBC-Iod-CBT–treated, NTR-overexpressing HeLa cells showed the existence of Iod-CBT-NPs in their cytoplasm with an average LAC value of 0.172 ± 0.032 μm −1 . We anticipate that our strategy could help people to deeply understand the formation mechanism of intracellular nanostructures in the near future.
Type of Medium:
Online Resource
ISSN:
2375-2548
DOI:
10.1126/sciadv.aba3190
Language:
English
Publisher:
American Association for the Advancement of Science (AAAS)
Publication Date:
2020
detail.hit.zdb_id:
2810933-8
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