In:
Oncology Research Featuring Preclinical and Clinical Cancer Therapeutics, Computers, Materials and Continua (Tech Science Press), Vol. 22, No. 1 ( 2014-10-23), p. 57-65
Abstract:
The potassium (K + ) channel plays an important role in the cell cycle and proliferation of tumor cells, while its role in brain glioma cells and the signaling pathways remains unclear. We used tetraethylammonium (TEA), a nonselective antagonist of big conductance K + channels, to block K + channels in glioma cells, and antioxidant N -acetyl-l-cysteine (NAC) to inhibit production of intracellular reactive oxygen species (ROS). TEA showed an antiproliferation effect on C6 and U87 glioma cells in a time-dependent manner, which was accompanied
by an increased intracellular ROS level. Antioxidant NAC pretreatment reversed TEA-mediated antiproliferation and restored ROS level. TEA treatment also caused significant increases in mRNA and protein levels of tumor-suppressor proteins p53 and p21, and the upregulation was attenuated by pretreatment of NAC. Our results suggest that K + channel activity significantly contributes to brain glioma cell proliferation via increasing ROS, and it might be an upstream factor triggering the activation of the p53/p21 Cip1 -dependent signaling pathway, consequently
leading to glioma cell cycle arrest.
Type of Medium:
Online Resource
ISSN:
0965-0407
DOI:
10.3727/096504014X14098532393518
Language:
English
Publisher:
Computers, Materials and Continua (Tech Science Press)
Publication Date:
2014
detail.hit.zdb_id:
1114699-0
detail.hit.zdb_id:
2044620-2
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