In:
PLOS ONE, Public Library of Science (PLoS), Vol. 16, No. 9 ( 2021-9-10), p. e0257248-
Abstract:
Meiosis is a complex process involving the expression and interaction of numerous genes in a series of highly orchestrated molecular events. Fam9b localized in Xp22.3 has been found to be expressed in testes. However, FAM9B expression, localization, and its role in meiosis have not been previously reported. In this study, FAM9B expression was evaluated in the human testes and ovaries by RT-PCR, qPCR, and western blotting. FAM9B was found in the nuclei of primary spermatocytes in testes and specifically localized in the synaptonemal complex (SC) region of spermatocytes. FAM9B was also evident in the follicle cell nuclei and diffusely dispersed in the granular cell cytoplasm. FAM9B was partly co-localized with SYCP3, which is essential for both formation and maintenance of lateral SC elements. In addition, FAM9B had a similar distribution pattern and co-localization as γH2AX, which is a novel biomarker for DNA double-strand breaks during meiosis. All results indicate that FAM9B is a novel meiosis-associated protein that is co-localized with SYCP3 and γH2AX and may play an important role in SC formation and DNA recombination during meiosis. These findings offer a new perspective for understanding the molecular mechanisms involved in meiosis of human gametogenesis.
Type of Medium:
Online Resource
ISSN:
1932-6203
DOI:
10.1371/journal.pone.0257248
DOI:
10.1371/journal.pone.0257248.g001
DOI:
10.1371/journal.pone.0257248.g002
DOI:
10.1371/journal.pone.0257248.g003
DOI:
10.1371/journal.pone.0257248.g004
DOI:
10.1371/journal.pone.0257248.g005
DOI:
10.1371/journal.pone.0257248.g006
DOI:
10.1371/journal.pone.0257248.t001
DOI:
10.1371/journal.pone.0257248.s001
DOI:
10.1371/journal.pone.0257248.s002
DOI:
10.1371/journal.pone.0257248.s003
DOI:
10.1371/journal.pone.0257248.s004
DOI:
10.1371/journal.pone.0257248.s005
DOI:
10.1371/journal.pone.0257248.s006
DOI:
10.1371/journal.pone.0257248.r001
DOI:
10.1371/journal.pone.0257248.r002
DOI:
10.1371/journal.pone.0257248.r003
DOI:
10.1371/journal.pone.0257248.r004
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2021
detail.hit.zdb_id:
2267670-3
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