In:
International Journal of Systematic and Evolutionary Microbiology, Microbiology Society, Vol. 65, No. Pt_10 ( 2015-10-01), p. 3703-3708
Abstract:
A novel Gram-stain-positive, aerobic, motile, non-spore-forming coccus-shaped actinomycete, designated strain YIM 121300 T , was isolated from alkaline sediment in Yuanjiang, Yunnan province, south-west China. Phylogenetic analyses based on the 16S rRNA gene sequence showed that strain YIM 121300 T was affiliated to the genus Kineococcus , and was closely related to Kineococcus aurantiacus IFO 15268 T (97.3 % similarity). 16S rRNA gene sequence similarity to other species of the genus Kineococcus was 〈 97 %. The cell wall contained meso -diaminopimelic acid as the diagnostic diamino acid. The whole-cell sugars contained arabinose, galactose, glucose, mannose and ribose. The predominant menaquinone was MK-9(H 2 ). Mycolic acids were not detected. The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannoside and an unknown phospholipid. The predominant fatty acids were anteiso-C 15 : 0 and anteiso-C 15 : 1 A. The G+C content of the genomic DNA was 75.1 mol%. DNA–DNA relatedness (55 ± 4 % to K. aurantiacus IFO 15268 T ) and differential phenotypic data demonstrated that strain YIM 121300 T was distinguished from all related species of the genus Kineococcus . On the basis of data from the present polyphasic study, the organism should be assigned to a novel species of the genus Kineococcus , for which the name Kineococcus gypseus sp. nov. is proposed. The type strain is YIM 121300 T ( = CCTCC AA 2013232 T = DSM 27627 T ).
Type of Medium:
Online Resource
ISSN:
1466-5026
,
1466-5034
DOI:
10.1099/ijsem.0.000478
Language:
English
Publisher:
Microbiology Society
Publication Date:
2015
detail.hit.zdb_id:
215062-1
detail.hit.zdb_id:
2056611-6
SSG:
12
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