In:
The Korean Journal of Parasitology, Korean Society for Parasitology, Vol. 60, No. 5 ( 2022-10-21), p. 345-352
Abstract:
Chitinase AO-801 is a hydrolase secreted by 〈 i 〉 Arthrobotrys oligospora 〈 /i 〉 during nematode feeding, while its role remained elusive. This study analyzed the molecular characteristics of recombinant chitinase of 〈 i 〉 Arthrobotrys oligospora 〈 /i 〉 (reAO-801). AO-801 belongs to the typical glycoside hydrolase 18 family with conserved chitinase sequence and tertiary structure of (α/β) 〈 sub 〉 8 〈 /sub 〉 triose-phosphate isomerase (TIM) barrel. The molecular weight of reAO-801 was 42 kDa. reAO-801 effectively degraded colloidal and powdered chitin, egg lysate, and stage I larval lysate of 〈 i 〉 Caenorhabditis elegans 〈 /i 〉 . The activity of reAO-801 reached its peak at 40˚C and pH values between 4-7. Enzyme activity was inhibited by Zn2 〈 sup 〉 + 〈 /sup 〉 , Ca2 〈 sup 〉 + 〈 /sup 〉 , and Fe3 〈 sup 〉 + 〈 /sup 〉 , whereas Mg2 〈 sup 〉 + 〈 /sup 〉 and K 〈 sup 〉 + 〈 /sup 〉 potentiated its activity. In addition, urea, sodium dodecyl sulfate, and 2-mercaptoethanol significantly inhibited enzyme activity. reAO-801 showed complete nematicidal activity against 〈 i 〉 C. elegans 〈 /i 〉 stage I larvae. reAO-801 broke down the 〈 i 〉 C. elegans 〈 /i 〉 egg shells, causing them to die or die prematurely by hatching the eggs. It also invoked degradation of 〈 i 〉 Haemonchus contortus 〈 /i 〉 eggs, resulting in apparent changes in the morphological structure. This study demonstrated the cytotoxic effect of reAO-801, which laid the foundation for further dissecting the mechanism of nematode infestation by 〈 i 〉 A. oligospora 〈 /i 〉 .
Type of Medium:
Online Resource
ISSN:
0023-4001
,
1738-0006
DOI:
10.3347/kjp.2022.60.5.345
Language:
English
Publisher:
Korean Society for Parasitology
Publication Date:
2022
detail.hit.zdb_id:
2163174-8
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