In:
Journal of Leukocyte Biology, Oxford University Press (OUP), Vol. 53, No. 5 ( 1993-05-01), p. 576-582
Abstract:
The expression of non-β2 integrins on polymorphonuclear neutrophils (PMNs) was analyzed by im- munoprecipitation and flow cytometry using platelet-free PMN preparations and anti-FcγR blocking mAbs. No β3 integrin was detected with six anti-β3 mAbs. Conversely, integrin β1 chain was present on PMNs, although at low level, and could be distinguished from platelet β1 by SDS-PAGE. The MW differences disappeared after N- glycanase treatment. PMNs express only 2500 molecules of β1 per cell and this expression is not modulated by agonists such as phorbol myristate acetate, formylmethionyl- leucyl-phenylalanine, granulocyte-macrophage colony- stimulating factor, or tumor necrosis factor α, which enhance CDllb expression, or by interferon-7 or transforming growth factor α. PMNs were found to express α6 associated with β1, and no reactivity was observed with various anti-α1, anti-α2, anti-α3, anti-α4, anti-α5 or anti-αv mAbs. In conclusion, although other leukocytes express various β1 integrins, which mediate cell interactions with ECM proteins, PMNs appear to express only the laminin receptor α6β1 PMN interactions with nonlaminin ECM ligands thus seem to be mediated either exclusively by β2 integrins or by nonintegrin molecules. J. Leukoc. Biol. 53: 576–582; 1993.
Type of Medium:
Online Resource
ISSN:
0741-5400
,
1938-3673
DOI:
10.1002/jlb.53.5.576
Language:
English
Publisher:
Oxford University Press (OUP)
Publication Date:
1993
detail.hit.zdb_id:
2026833-6
SSG:
12
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