In:
Medicinal Research Reviews, Wiley, Vol. 39, No. 2 ( 2019-03), p. 665-683
Abstract:
The system of clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR‐associated endonucleases (Cas) has been utilized for genome editing with great accuracy and high efficiency in generating gene knockout, knockin, and point mutations in eukaryotic genomes. However, traditional CRISPR/Cas9 technology introduces double‐stranded DNA breaks (DSBs) at a target locus as the first step to make gene corrections, which easily results in undesired mutations. Thus, it is necessary to develop new methods for correcting the unwanted mutations. In this review, we summarize the recent developments and a new approach to genome and base editing by using CRISPR/Cas9. This methodology renders a conversion of one target base into another, for example, C to T (or G to A), and A to G (or T to C) without producing DSBs, requiring a donor DNA template, or generating excessive insertions and deletions. Furthermore, CRISPR/Cas9‐derived base editing also improves efficiency in repairing point mutations in the genome.
Type of Medium:
Online Resource
ISSN:
0198-6325
,
1098-1128
DOI:
10.1002/med.2019.39.issue-2
Language:
English
Publisher:
Wiley
Publication Date:
2019
detail.hit.zdb_id:
2001841-1
SSG:
15,3
Permalink