In:
Angewandte Chemie, Wiley, Vol. 134, No. 36 ( 2022-09-05)
Abstract:
T and B lymphocytes are two major adaptive immune cells in the human defense system. To real‐time monitor their diverse functions, a live‐cell‐selective probe for only one cell type is need to investigate the complex interaction of the immune cells. Herein, a small‐molecule probe CDyB for live B cells is developed by an unbiased fluorescence library screening. The cell selectivity was confirmed by multiparametric single‐cell analysis using CyTOF. Through a systematic SLC‐CRISPRi library screening, the molecular target of CDyB was identified as SLC35C2 transporter based on a gating‐oriented live‐cell distinction (GOLD) mechanism. The gene expression analysis and knock‐out experiments validated that the SLC35C2 transporter was the target for CDyB distinction. Interestingly, when CDyB was applied to study B cell development, the CDyB fluorescence and SLC35C2 expression were positively correlated with the B cell maturation process, and not involved in the T cell development.
Type of Medium:
Online Resource
ISSN:
0044-8249
,
1521-3757
DOI:
10.1002/ange.v134.36
DOI:
10.1002/ange.202202095
Language:
English
Publisher:
Wiley
Publication Date:
2022
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