In:
Frontiers in Plant Science, Frontiers Media SA, Vol. 13 ( 2022-11-1)
Abstract:
Several genes regulating capsaicinoid biosynthesis including Pun1 (also known as CS ), Pun3 , pAMT , and CaKR1 have been studied. However, the gene encoded by Pun2 in the non-pungent Capsicum chacoense is unknown. This study aimed to identify the Pun2 gene by genetic mapping using interspecific ( C. chacoense × Capsicum annuum ) and intraspecific ( C. chacoense × C. chacoense ) populations. QTL mapping using the interspecific F 2 population revealed two major QTLs on chromosomes 3 and 9. Two bin markers within the QTL regions on two chromosomes were highly correlated with the capsaicinoid content in the interspecific population. The major QTL, Pun2_PJ_Gibbs_3.11 on chromosome 3, contained the pAMT gene, indicating that the non-pungency of C. chacoense may be attributed to a mutation in the pAMT gene. Sequence analysis revealed a 7 bp nucleotide insertion in the 8 th exon of pAMT of the non-pungent C. chacoense . This mutation resulted in the generation of an early stop codon, resulting in a truncated mutant lacking the PLP binding site, which is critical for pAMT enzymatic activity. This insertion co-segregated with the pungency phenotype in the intraspecific F 2 population. We named this novel pAMT allele pamt 11 . Taken together, these data indicate that the non-pungency of C. chacoense is due to the non-functional pAMT allele, and Pun2 encodes the pAMT gene.
Type of Medium:
Online Resource
ISSN:
1664-462X
DOI:
10.3389/fpls.2022.1039393
DOI:
10.3389/fpls.2022.1039393.s001
DOI:
10.3389/fpls.2022.1039393.s002
DOI:
10.3389/fpls.2022.1039393.s003
DOI:
10.3389/fpls.2022.1039393.s004
DOI:
10.3389/fpls.2022.1039393.s005
DOI:
10.3389/fpls.2022.1039393.s006
DOI:
10.3389/fpls.2022.1039393.s007
DOI:
10.3389/fpls.2022.1039393.s008
DOI:
10.3389/fpls.2022.1039393.s009
Language:
Unknown
Publisher:
Frontiers Media SA
Publication Date:
2022
detail.hit.zdb_id:
2687947-5
detail.hit.zdb_id:
2613694-6
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