In:
Pathobiology, S. Karger AG, Vol. 89, No. 2 ( 2022), p. 116-126
Abstract:
〈 b 〉 〈 i 〉 Background/Aims: 〈 /i 〉 〈 /b 〉 The presence and clinical importance of tissue-resident memory T (T 〈 sub 〉 RM 〈 /sub 〉 ) cells have been recently described in association with various cancer types. However, the frequency and the traditional naïve–effector–memory phenotypic characteristics of T 〈 sub 〉 RM 〈 /sub 〉 cells are largely unknown. 〈 b 〉 〈 i 〉 Methods: 〈 /i 〉 〈 /b 〉 We analyzed single-cell populations of colorectal cancer (CC, 〈 i 〉 n 〈 /i 〉 = 18), stomach cancer (SC, 〈 i 〉 n 〈 /i 〉 = 13), renal cell carcinoma (RCC, 〈 i 〉 n 〈 /i 〉 = 19), and breast cancer (BC, 〈 i 〉 n 〈 /i 〉 = 16) by dissociation of tumor tissue with collagenase/hyaluronidase. We investigated populations of naïve, effector, and memory T and T 〈 sub 〉 RM 〈 /sub 〉 cells by flow cytometry. 〈 b 〉 〈 i 〉 Results: 〈 /i 〉 〈 /b 〉 Among CD8 〈 sup 〉 − 〈 /sup 〉 cells, CC was associated with a significantly higher proportion of CD103 〈 sup 〉 + 〈 /sup 〉 T cells than other tumor types ( 〈 i 〉 p 〈 /i 〉 & #x3c; 0.001). Among CD8 〈 sup 〉 + 〈 /sup 〉 cells, CC and SC were associated with higher CD103 〈 sup 〉 + 〈 /sup 〉 T-cell proportions than RCC and BC ( 〈 i 〉 p 〈 /i 〉 & #x3c; 0.001). Significantly more CD8 〈 sup 〉 + 〈 /sup 〉 than CD8 〈 sup 〉 − 〈 /sup 〉 cells expressed CD103 ( 〈 i 〉 p 〈 /i 〉 & #x3c; 0.001). In association with SC, RCC, and BC, CD8 〈 sup 〉 + 〈 /sup 〉 T cells had a similar T-cell phenotype composition pattern: fewer effector T cells and more memory-type T cells among CD103 〈 sup 〉 + 〈 /sup 〉 cells compared with CD103 〈 sup 〉 − 〈 /sup 〉 cells ( 〈 i 〉 p 〈 /i 〉 & #x3c; 0.05). Tumors with higher proportion of CD103 〈 sup 〉 + 〈 /sup 〉 cells had no specific clinicopathologic characteristics than those with lower proportion of CD103 〈 sup 〉 + 〈 /sup 〉 cells. 〈 b 〉 〈 i 〉 Conclusion: 〈 /i 〉 〈 /b 〉 T 〈 sub 〉 RM 〈 /sub 〉 cell abundance and phenotypes varied among CC, SC, RCC, and BC. Further studies regarding the functional differences of T 〈 sub 〉 RM 〈 /sub 〉 associated with various tumors are warranted.
Type of Medium:
Online Resource
ISSN:
1015-2008
,
1423-0291
Language:
English
Publisher:
S. Karger AG
Publication Date:
2022
detail.hit.zdb_id:
1483541-1
SSG:
12
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