In:
Journal of Medical Microbiology, Microbiology Society, Vol. 55, No. 12 ( 2006-12-01), p. 1661-1665
Abstract:
Salmonella enterica subspecies enterica serovar Paratyphi B [O1,4,(5),12 : Hb : 1,2] can cause either an enteric fever (paratyphoid fever) or self-limiting gastroenteritis in humans. The d -tartrate non-fermenting variant S. enterica subsp. enterica serovar Paratyphi B dT − ( S . Paratyphi B) is the causative agent of paratyphoid fever, and the d -tartrate fermenting variant S. enterica subsp. enterica serovar Paratyphi B dT + ( S . Paratyphi B dT + ; formerly called Salmonella Java) causes gastroenteritis. S . Java is currently recognized as an emerging problem worldwide. Twelve dT + S . Java isolates were collected in Indonesia between 2000 and 2002. One-third of them contained Salmonella genomic island 1 (SGI1), which gives the multidrug-resistant phenotype to the bacteria. In this study, a PCR-based method to detect a single nucleotide difference responsible for the inability to ferment d -tartrate, reported elsewhere, was validated. The d -tartrate fermenting phenotype of S . Java was converted to the non-fermenting phenotype by the disruption of the ORF STM 3356, and the d -tartrate non-fermenting phenotype of the ORF STM 3356-disrupted strain and the dT − reference strain was changed to the dT + phenotype by complementing ORF STM 3356 in trans . The results show that the dT + phenotype requires a functional product encoded by STM 3356, and support the use of the PCR-based discrimination method for S . Paratyphi B and S . Java as the standard differentiation method.
Type of Medium:
Online Resource
ISSN:
0022-2615
,
1473-5644
DOI:
10.1099/jmm.0.46792-0
Language:
English
Publisher:
Microbiology Society
Publication Date:
2006
detail.hit.zdb_id:
2083944-3
SSG:
12
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